Antibacterial microemulsion prevents sepsis and triggers healing of wound in wistar rats

Ghosh, Vijayalakshmi; Saranya, S.; Mukherjee, Amitava; Chandrasekaran, Natarajan

doi:10.1016/j.colsurfb.2013.01.009

Cited by 83 publications

(32 citation statements)

References 37 publications

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“…Thermodynamic stability studies of all prepared emulsions were performed according to Ghosh et al ().…”

Section: Methodsmentioning

confidence: 99%

Formulation and characterization of betel leaf (Piper betleL.) essential oil based nanoemulsion and its in vitro antibacterial efficacy against selected food pathogens

Guha

2018

J Food Process Preserv

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The current study is focused on the formulation and characterization of oil‐in‐water nanoemulsion of betel leaf (Piper betle L.) essential oil and its antibacterial efficacy. The nanoemulsion consists of essential oil of betel leaf as the dispersed phase, distilled water as a continuous phase and a nonionic surfactant Tween 20 (Polysorbate 20) as an emulsifier. Six emulsions were formulated varying the oil to surfactant ratio (vol/vol) from 1:1 to 1:6, using high‐energy emulsification process. Three samples, that is, S2 = 1:2 (3% EO, 6% Tween 20), S3 = 1:3 (3% EO, 9% Tween 20), and S4 = 1:4 (3% EO, 12% Tween 20) shown droplet diameter of 50 ± 8, 70 ± 12, and 175 ± 7 nm, respectively. The formulated nanoemulsions showed antimicrobial activity as minimum inhibitory concentration (MIC) of 0.5–1.25 µl/ml and minimum bactericidal concentration (MBC) of 1–2.5 µl/ml against five strains of gram‐positive and gram‐negative bacteria. The formulated nanoemulsions thus can serve as natural antimicrobial agent for food system. Practical application This study can be useful in understanding the potential of essential oil from plant sources in food processing. It can also be helpful to formulate nanoemulsion from essential oil and its characterization. From characterization, researchers can get the knowledge about changes in the morphological characteristics of the nanoemulsion and also other physical properties. The thermodynamic and kinetic stability study of the nanoemulsions can give us an idea about the application in different food processing and preservation conditions. Furthermore, the use of essential oil in the food industry is mainly as a flavouring agent. Based on the antibacterial efficacy of the formulated nanoemulsions, it can also be utilized as a potential natural class one preservative.

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“…Thermodynamic stability studies of all prepared emulsions were performed according to Ghosh et al ().…”

Section: Methodsmentioning

confidence: 99%

Formulation and characterization of betel leaf (Piper betleL.) essential oil based nanoemulsion and its in vitro antibacterial efficacy against selected food pathogens

Guha

2018

J Food Process Preserv

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“…The topically applied microemulsion formulation was found to accelerate wound healing while also reducing the inflammatory response to chemical-induced corneal alkali burns in rats. A very low droplet diameter (5.7 nm) nanoemulsion based on cinnamon oil was developed for wound healing application and its stability and antibacterial efficacy was evaluated against S. aureus [47]. The microemulsion served as an antibacterial against S. aureus and the microemulsion treated group showed complete healing of wounds in 14 days whereas it took 16 days each for cinnamon oil only and standard ointment treated group, and 20 days for the untreated (control) animals.…”

Section: Nano and Micro-emulsionsmentioning

confidence: 99%

Recent developments in novel drug delivery systems for wound healing

Pachuau

2015

Expert Opinion on Drug Delivery

112

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Controlled and localized delivery of wound healing drugs to the wounds is more convenient than systemic administration as higher concentrations of the medication are delivered directly to the desired area in a sustained manner. They are also capable of providing optimum environmental conditions to facilitate wound healing while eliminating the need for frequent changes of dressings. As the number of people suffering from chronic wounds is increasing around the world, controlled delivery of wound healing agents have enormous potential for patient-friendly wound management.

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“…Nanoparticles are taken up by cells more efficiently than larger micromolecules and therefore, could be used as effective transport and delivery systems. The strategy of applying nanotechnology to plant extracts has been widely used, because nanostructure systems could increase effect of action of plant extracts, promote sustained release of active constituents, reduce the required dose, decrease side effects, and improve activity 12,13 . There are three different of the nanoparticles preparative.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

The Development of Centellaasiatica Extract-Loaded BSA Nanoparticles Production to Improve Bioavailability

Kesornbuakao¹,

Yasurin²

2016

Orient. J. Chem

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Centella asiatica (Bao-bog, Pennywort, Gotu kola) is used as a traditional drug widely in Asia. C. asiatica crude extracts showed excellent potential in-vitro but less or no in-vivo activity due to their poor lipid solubility or improper molecular size or both, resulting in poor absorption, poor dosing and poor bioavailability. The Bovine serum albumin (BSA) can attract macromolecular and carry wide variety of molecule. So this research was aimed to develop C. asiatica extract-loaded BSA nanoparticles (CBNP) to improve bioavailability. CBNP was prepared by the desolvation method using three different ratio C. asiatica crude chloroform extracts: BSA (1:2, 1:3, and 1:4). The well agar diffusion method was used for evaluating antibacterial activity of CBNP with different concentration (100, 200, and 300 µg/ml) against five food borne pathogens (Escherichia coli ATCC25822, Salmonella enterica Typhimurium U302 (DT104b), S. enterica Enteritidis (human), S. enterica 4,5,12:i:-(human) US clone, and Bacillus cereus). The results showed that the antibacterial activity of CBNP did not show significant different on three different ratio and concentration in all food borne pathogens except S. enterica Enteritidis (human) and B. cereus (P < 0.05). The highest antibacterial active of CBNP was 1.07±0.46 cm against S. enterica Enteritidis (human) on ratio 1:4, 200 µg/ml. The antibacterial activity of CBNP gave almost 2 times higher than free crude C. asiatica chloroform extracts. The modified Folin-Ciocalteu method, Ferric reducing antioxidant potential assay and DPPH assay were used for evaluating antioxidant activity. The highest antioxidant activity represented by the amount of phenolic content of CBNP was 14.59±6.74 µgGAE/mg using ratio 1:2. The amount of phenolic content of CBNP did not show significant different between ratio 1:2 and others (P < 0.05). For Ferric reducing antioxidant potential assay and DPPH assay, crude chloroform extract showed significantly higher activity than CBNP were 1.00 ± 0.15 mmol Fe 2+ /mg and 29.44 ± 8.20 % DPPH radical scavenging respectively (P < 0.05). The entrapment efficiency and loading efficiency of CBNP showed highest value on ratio 1:4 which were 96.94 ± 1.48% and 42.05 ± 5.68% respectively. It's also showed highest in release kinetic in Vitro approximately 12% during the whole period of 6 hours in both artificial gastric and intestinal juice. C. asiatica crude chloroform extract have higher solubility in water than CBNP but CBNP have higher stability in releasing crude chloroform extract. The results indicated that CBNP showed the promising to increase bioavailability of C. asiatica. The statistical analysis was done by ANOVA using SAS software version 9.3.

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Antibacterial microemulsion prevents sepsis and triggers healing of wound in wistar rats

Cited by 83 publications

References 37 publications

Formulation and characterization of betel leaf (Piper betleL.) essential oil based nanoemulsion and its in vitro antibacterial efficacy against selected food pathogens

Formulation and characterization of betel leaf (Piper betleL.) essential oil based nanoemulsion and its in vitro antibacterial efficacy against selected food pathogens

Recent developments in novel drug delivery systems for wound healing

The Development of Centellaasiatica Extract-Loaded BSA Nanoparticles Production to Improve Bioavailability

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