Antibacterial peptide NZ2114-loaded hydrogel accelerates Staphylococcus aureus-infected wound healing

Huang, Yan; Yang, Na; Teng, Da; Mao, Ruoyu; Hao, Ya; Ma, Xuanxuan; Wei, Lingyun; Wang, Jianhua

doi:10.1007/s00253-022-11943-w

Cited by 20 publications

(18 citation statements)

References 62 publications

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“…Among the numerous hydrogel materials, hydroxypropyl cellulose is widely used in biomedical and engineering fields as hydrophilic polymer materials, which has been approved by the FDA. Hydroxypropyl cellulose is commonly used as a thickener, stabilizer, and drug-release agent in topical medicine due to its high water retention and water vapor transmission properties . Therefore, in the present study we selected hydroxypropyl cellulose as the material to prepare the peptide gels.…”

Section: Resultsmentioning

confidence: 99%

“…Hydroxypropyl cellulose is commonly used as a thickener, stabilizer, and drug-release agent in topical medicine due to its high water retention and water vapor transmission properties. 42 Therefore, in the present study we selected hydroxypropyl cellulose as the material to prepare the peptide gels. Considering the sustained drug release property of hydroxypropyl cellulose, in order to acquire enough drug release in a short time, the peptides were dissolved in 0.5% hydroxypropyl cellulose to a high concentration of 2 mg/mL to prepare the peptide gels.…”

Section: Peptides With a More Rapid Bacterial Killing Rate Possess Be...mentioning

confidence: 99%

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Hydrophobicity Determines the Bacterial Killing Rate of α-Helical Antimicrobial Peptides and Influences the Bacterial Resistance Development

Zhang

Ouyang

et al. 2022

J. Med. Chem.

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Rapid antimicrobial action is an important advantage of antimicrobial peptides (AMPs) over antibiotics, which is also a reason for AMPs being less likely to induce bacterial resistance. However, the structural parameters and underlying mechanisms affecting the bacterial killing rate of AMPs remain unknown. In this study, we performed a structure−activity relationship (SAR) study using As-CATH4 and 5 as templates. We revealed that hydrophobicity, rather than other characteristics, is the critical structural parameter determining the bacterial killing rate of α-helical AMPs. With the hydrophobicity increase, the action rates of AMPs including bacterial binding, lipopolysaccharides neutralization, and outer and inner membrane permeabilization increased. Additionally, the higher hydrophobic AMPs with enhanced bacterial killing rates possess better in vivo therapeutic potency and a lower propensity to induce bacterial resistance. These findings revealed the importance of the bacterial killing rate for AMPs and are of great significance to the design and optimization of AMP-related drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Peptides With a More Rapid Bacterial Killing Rate Possess Be...mentioning

confidence: 99%

Hydrophobicity Determines the Bacterial Killing Rate of α-Helical Antimicrobial Peptides and Influences the Bacterial Resistance Development

Zhang

Ouyang

et al. 2022

J. Med. Chem.

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“… 17 In addition, hydroxypropyl cellulose/SA hydrogel loaded with antibacterial peptide NZ2114 was confirmed to accelerate wound healing by promoting the expression of VEGF, EGF, and CD31 as well as reducing inflammatory response. 18 These studies demonstrate that hydrogels encapsulating bioactive peptides/proteins seem to be a promising research direction for wound dressings. However, it is currently insufficient to deeply explore the biological mechanism by which hydrogels accelerate wound repair.…”

Section: Introductionmentioning

confidence: 91%

Chitosan/Sodium Alginate/Velvet Antler Blood Peptides Hydrogel Promotes Diabetic Wound Healing via Regulating Angiogenesis, Inflammatory Response and Skin Flora

Hao

Ding

Sun

et al. 2022

JIR

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Background Diabetic ulcer remains a clinical challenge due to impaired angiogenesis and persistent inflammation, requiring new alternative therapies to promote tissue regeneration. Purpose In this study, chitosan/sodium alginate/velvet antler blood peptides (CS/SA/VBPs) hydrogel (CAVBPH) was fabricated and used in the treatment of skin wounds in type 2 diabetes mellitus (T2D) for the first time. Methods VBPs were prepared by hydrolysis and ultrafiltration, and their sequences were identified using LC-MS/MS. The CAVBPH was further fabricated and characterized. A mouse model of T2D was induced by a high-sugar and high-fat diet (HSFD) and streptozotocin (STZ) injection. CAVBPH was applied topically to T2D wounds, and its effects on skin repair and potential biological mechanisms were analyzed by appearance observation, histopathological staining, bioinformatics analysis, Western blot, and 16S rRNA sequencing. Results VBPs had numerous short-chain active peptides, excellent antioxidant activity, and a low hemolysis rate. CAVBPH exhibited desirable biochemical properties and participated in the diabetic wound healing process by promoting cell proliferation (PCNA and α-SMA) and angiogenesis (capillaries and CD31) and alleviating inflammation (CD68). Mechanistically, the therapeutic effect of CAVBPH on chronic wounds might rely on activating the PI3K/AKT/mTOR/HIF-1α/VEGFA pathway and reversing the expression of inflammatory cytokines TNF-α and IL-1β. The results of 16S rRNA sequencing indicated that T2D significantly altered the diversity and structure of skin flora at the wound site. CAVBPH treatment elevated the relative abundance of beneficial microbes (e.g., Corynebacterium_1 and Lactobacillus ) and reversed the structural imbalance of skin microbiota. Conclusion These results indicate that CAVBPH is a promising wound dressing, and its repair effect on diabetic wounds by regulating angiogenesis, inflammatory response, and skin flora may depend on the rich small peptides in VBPs.

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“…On day 14, wounds in the negative group did not heal, or festered, whereas the treated group wounds were free of bacteria. 183 In conclusion, hydrogels can be combined with different biological substances or drugs in various ways to reduce the risk of tissue infection with bacteria and achieve a better effect of promoting tissue repair.…”

Section: Functions Of Hydrogel In Tissue Regeneration and Repairmentioning

confidence: 99%

The diversified hydrogels for biomedical applications and their imperative roles in tissue regeneration

Wang

et al. 2023

Biomater. Sci.

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Antibacterial peptide NZ2114-loaded hydrogel accelerates Staphylococcus aureus-infected wound healing

Cited by 20 publications

References 62 publications

Hydrophobicity Determines the Bacterial Killing Rate of α-Helical Antimicrobial Peptides and Influences the Bacterial Resistance Development

Hydrophobicity Determines the Bacterial Killing Rate of α-Helical Antimicrobial Peptides and Influences the Bacterial Resistance Development

Chitosan/Sodium Alginate/Velvet Antler Blood Peptides Hydrogel Promotes Diabetic Wound Healing via Regulating Angiogenesis, Inflammatory Response and Skin Flora

The diversified hydrogels for biomedical applications and their imperative roles in tissue regeneration

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