Antibacterial Potential of Soft Coral Lobophytum sp. From Pangalisang Waters of Bunaken Island to Pseudomonas aeruginosa and Staphylococcus aure

Kowal, Abraham L.; Angkouw, Esther D.; Kawung, Nickson J.; Khemer, Kurniati; Manoppo, Henky; Sumilat, Deiske A.

doi:10.35800/jip.6.2.2018.20638

Cited by 2 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Salah satu peran ekosistem terumbu karang adalah marine natural product (MNP) yang berasal dari metabolit sekunder organisme laut. Organisme laut penyusun ekosistem terumbu karang yang memiliki metabolit sekunder di antaranya adalah karang lunak (Patra & Majumdar, 2004;Tanod et al, 2015;Kawung et al, 2017;Kowal et al, 2018), alga (Tanod et al, 2015), spons (Luissandy et al, 2017;, dan Ascidian (Sumilat et al, 2017;. Karang lunak Nephthea sp.…”

Section: Pendahuluanunclassified

“…Karang lunak yang berasal dari habitat aslinya memiliki kandungan metabolit sekunder dari golongan benzena (Tanod et al, 2019) dan terbukti memiliki potensi antibakteri (Kowal et al, 2018) serta berpotensi sebagai bahan baku obat (Apri et al, 2013). Namun, identifikasi metabolit sekunder dari karang lunak Nephthea sp.…”

Section: Pendahuluanunclassified

“…Senyawa alkaloid, tanin, dan steroid jika dikonsumsi oleh serangga, maka senyawa-senyawa tersebut dapat menjadi racun perut bagi serangga dan menyebabkan kematian pada larvanya (Javandira et al, 2016). Beberapa karang lunak telah terbukti menghasilkan senyawa aktif seperti antibakteri (Kowal et al, 2018), antikanker, antiinflamasi (Fattorusso et al, 2009) dan memiliki potensi dalam pengobatan kolestasis (Putra et al, 2016).…”

Section: Aktivitas Antioksidan Nephthea Spunclassified

See 2 more Smart Citations

Identifikasi Metabolit Sekunder dan Aktivitas Antioksidan Karang Lunak Nephthea sp. Hasil Transplantasi Secara In Situ dan Ex Situ

Fahrudin,

Haribowo,

Karmila

et al. 2023

Al-Kauniyah J. Biol.

View full text Add to dashboard Cite

AbstrakKarang lunak (Nephthea sp.) menghasilkan metabolit sekunder di habitat aslinya dan dapat dikembangkan menjadi marine natural product (MNP). Nephthea sp. hasil transplantasi diharapkan juga menghasilkan metabolit sekunder yang sama. Tujuan penelitian ini adalah melakukan identifikasi metabolit sekunder secara kualitatif dan kuantitatif pada Nephthea sp. hasil transplantasi serta menguji aktivitas antioksidannya. Sampel Nephthea sp. berasal dari Taman Nasional Ujung Kulon (in situ) dan dari akuarium sebagai sampel transplantasi ex situ. Kedua sampel diekstraksi dengan maserasi menggunakan etanol 70% dengan perbandingan 1:3 (w/v). Identifikasi metabolit sekunder dilakukan secara kualitatif dan metode Gas Chromatography-Mass Spectrometry (GC-MS) untuk identifikasi kuantitatif. Aktivitas antioksidan menggunakan uji DPPH (2,2-difenil-1-1 pikrilhidrazil) dengan lima konsentrasi dari setiap sampel (10 ppm, 50 ppm, 100 ppm, 150 ppm, dan 200 ppm). Nephthea sp. hasil transplantasi memiliki metabolit sekunder golongan alkaloid, tanin, dan steroid. Hasil uji GC-MS menunjukkan terdapat enam jenis senyawa aktif golongan benzene dan fatty acid. Aktivitas antioksidan diperoleh 34,3–73,1% (in situ) dan 33,2–72,3% (ex situ) serta berbeda nyata (P <0,05) pada setiap konsentrasi dari dua sampel. Peningkatan aktivitas antioksidan Nephthea sp. tidak berbeda signifikan (P >0,05) pada perbedaan lokasi transplantasi (in situ dan ex situ). Nephthea sp. hasil transplantasi mengandung enam jenis senyawa metabolit sekunder dan memiliki aktivitas antioksidan.AbstractSoft corals (Nephthea sp.) produce secondary metabolites in their habitat and can be used as marine natural products (MNP). However, Nephthea sp. transplantation not done identified for secondary metabolites. The aim research to identify secondary metabolites and antioxidant activity assay in Nephthea sp. transplant. Samples of Nephthea sp. used from the transplant area of Ujung Kulon National Park (in situ) and from the aquarium as ex situ transplant samples. Both samples extracted by maceration with 70% ethanol (1:3, w/v). Identification of secondary metabolites was carried out qualitatively and using the GC-MS method for quantitative. Antioxidant activity using the DPPH (2,2-diphenyl-1-1 picrylhydrazyl) assay. with concentrations of each sample (10 ppm, 50 ppm, 100 ppm, 150 ppm, and 200 ppm). Nephthea sp. proven to have secondary metabolites from the alkaloids, tannins and steroids. The result of GC-MS showed that six types of active compounds from the benzene and fatty acid groups. Antioxidant activity obtained was 34.3–73.1% (in situ) dan 33.2–72.3% (ex situ) and was significantly different (P <0.05) at each concentration of the two samples. Antioxidant activity at different transplant locations (in situ and ex situ) in Nephthea sp. did not a significant (P >0.05). Thus, Nephthea sp. transplant were proven to contain six types of secondary metabolite compounds and has antioxidant activity.

show abstract

Section: Pendahuluanunclassified

Section: Aktivitas Antioksidan Nephthea Spunclassified

See 1 more Smart Citation

Identifikasi Metabolit Sekunder dan Aktivitas Antioksidan Karang Lunak Nephthea sp. Hasil Transplantasi Secara In Situ dan Ex Situ

Fahrudin,

Haribowo,

Karmila

et al. 2023

Al-Kauniyah J. Biol.

View full text Add to dashboard Cite

show abstract

“…Lobophytum sp. showed as cytotoxic (Fattorusso et al, 2009) and antibacterial agent (Kowal et al, 2018). Soft coral Sarcophyton sp.…”

Section: Introductionmentioning

confidence: 99%

Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

Tanod

Dewanto

Ndobe

et al. 2019

Squalen Bull. Marine Fisheries Postharvest Biotech.

View full text Add to dashboard Cite

This study aimed to evaluate the potential antibacterial and antioxidant activities of Sinulariasp. and Sarcophyton sp. from the Palu Bay, Central Sulawesi, Indonesia. Soft corals were identified as Sinulariasp. (SC1), Sinularia sp. (SC2), andSarcophytonsp. (SC3). Antibacterial activity was examined using agar diffusion well method. Antioxidant activity was measured by the DPPH radical scavenging method. The samples were macerated in MeOH: DCM. The crude extracts were partitioned with DCM, EtOAc, and BuOH. The crude extract of Sinulariasp. (SC2) showed a very strong antibacterial activity as it was able to inhibit the growth of Staphylococcus aureusand Escherichia coliup to 10 mg/mL. Sinularia sp. (SC1) crude extract showed strong activity againstS. aureus, whereas it showed moderate against E. coli.Sarcophyton sp. (SC3) crude extract showed moderate activity against S. aureus, whereas it showed weak against E. coli. The partition fractions of the three soft coral extracts had the potential to be a potent antioxidant agent.

show abstract

Antibacterial Potential of Soft Coral Lobophytum sp. From Pangalisang Waters of Bunaken Island to Pseudomonas aeruginosa and Staphylococcus aure

Cited by 2 publications

References 5 publications

Identifikasi Metabolit Sekunder dan Aktivitas Antioksidan Karang Lunak Nephthea sp. Hasil Transplantasi Secara In Situ dan Ex Situ

Identifikasi Metabolit Sekunder dan Aktivitas Antioksidan Karang Lunak Nephthea sp. Hasil Transplantasi Secara In Situ dan Ex Situ

Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

Contact Info

Product

Resources

About