Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

Tanod, Wendy Alexander; Dewanto, Didit Kustantio; Ndobe, Samliok; Riyadi, Putut Har; Putra, Masteria Yunovilsa

doi:10.15578/squalen.v14i2.394

Cited by 10 publications

(13 citation statements)

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“…The DPPH antioxidant assay is the most popular tool to evaluate a substance’s ability to inhibit free radicals because it is simple, sensitive, and a fast way to test antioxidants [ 36 , 37 ]. The DPPH reduction measured in the TVHE samples was proportional to the amount of purple solution turning yellow to calculate an IC 50 value, suggesting that 50% of the DPPH-free radicals are scavenged in solution [ 33 ]. Table-1 shows the IC 50 for the four TVHEs.…”

Section: Discussionmentioning

confidence: 99%

“…DOCA-salt induction increases free radicals, which liberating renal phospholipids. These lipids are further broken down by peroxide to produce MDA [ 33 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH, Merck) radical scavenging method [ 32 , 33 ]. Twenty-five milligrams of TVHE were added to methanol at an extract concentration of 200 μg/mL.…”

Section: Methodsmentioning

confidence: 99%

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Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

et al. 2020

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Background and Aim: Hypertension is closely related to oxidative stress conditions, which increases malondialdehyde (MDA) expression and renal damage. Tilapia viscera hydrolysate extract (TVHE) contains compounds and peptides that act as antioxidants. This study aimed to investigate TVHE therapy effect on MDA levels and renal histological conditions in deoxycorticosterone acetate (DOCA)-salt-induced hypertension rats. Materials and Methods: Tilapia viscera were defatted and hydrolyzed using Alcalase enzyme to obtain TVHE. TVHE antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl method. Fifteen Wistar male rats were divided into five groups: Normal control (without induced DOCA-salt), DOCA-salt, DOCA-salt+Captopril 5 mg/kg body weight (BW), DOCA-salt+TVHE 150 mg/kg BW, and DOCA-salt+TVHE 300 mg/kg BW. MDA level and renal histology were observed in each group. Results: TVHE half maximal inhibitory concentration values ranged from 3.87±0.35 μg/mL to 42.03±3.55 μg/mL, which were identified as in the very strong Blois category. TVHE and captopril therapy reduced MDA expression significantly (p<0.05) compared to DOCA-salt only. TVHE and captopril therapy also improved glomerular damage in DOCA-salt-induced hypertension rats. Conclusion: TVHE has antioxidant ability, decreased MDA level, and decreased glomerular damage in DOCA-salt-induced hypertension rats.

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Section: Discussionmentioning

confidence: 99%

“…DOCA-salt induction increases free radicals, which liberating renal phospholipids. These lipids are further broken down by peroxide to produce MDA [ 33 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

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Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

et al. 2020

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“…Studi literatur menunjukkan Sinularia sp. memproduksi senyawa bioaktif dengan sifat antibakteri (Sun et al, 2012;Rozirwan et al, 2014;Rajaram et al, 2014;Putra et al, 2016b;and Tanod, 2019b). Selain itu, Sinularia sp.…”

Section: Pendahuluanunclassified

AKTIVITAS ANTIBAKTERI EKSTRAK KARANG LUNAK Sinularia sp. DENGAN METODE BROTH-DILUTION

Salanggon

Aswani

Hasanuddin

et al. 2020

Jurnal Kelautan Nasional

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Karang lunak merupakan bagian dari ekosistem terumbu karang, yang mengandung senyawa bioaktif dan dapat berperan sebagai alat pertahanan diri serta memiliki kemampuan sebagai senyawa antibakteri. Penelitian ini bertujuan untuk menginvestigasi potensi ekstrak karang lunak Sinularia sp. sebagai antibakteri dengan metode broth-dilution. Karang Lunak Sinularia sp. dikoleksi dari perairan Desa Kabonga Besar, Sulawesi Tengah, dan dimaserasi dengan diklorometana : methanol. Ekstrak Sinularia sp. dilakukan pengujian skrining metabolit kimia dan pengujian antibakteri dengan metode broth-diluttion. Hasil skrining ekstrak Sinularia sp. terdeteksi senyawa metabolit flavonoid, saponin, alkaloid dan steroid. Ekstrak Sinularia sp. memberikan pengaruh terhadap total koloni Escherichia coli dan Pseudomonas aeruginosa. Ekstrak Sinularia sp. dapat menghambat pertumbuhan E. coli dengan konsentrasi minimum 1.76 ± 0.10 mg/mL, sedangkan pada P. aeruginosa konsentrasi minimum yang dibutuhkan 1.85 ± 0.14 mg/mL. Konsentrasi bunuh minimum ekstrak Sinularia sp. yang dibutuhkan dalam membunuh E. coli, yaitu 7.06 ± 0.42 mg/mL, sedangkan pada P. aeruginosa, yaitu 7.38 ± 0.54 mg/mL. Penelitian ini disimpulkan bahwa senyawa bioaktif dari metabolit ekstrak karang lunak Sinularia sp. berpotensi sebagai senyawa antibakterial terhadap E. coli dan P. aeruginosa. Oleh karena itu, perlu diinvestigasi lebih lanjut dari identifikasi profil bioaktif karang lunak Sinularia sp.

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“…Sarcophyton sp. berpotensi inhibitor tumor necrosis factor- (Kapojos et al, 2010), inhibitor nuclear factor-kappa B dan inducible nitric oxide synthase (Riyadi, Wahyudi & Tanod, 2019), antioksidan (Tanod, Yanuhar, Maftuch, Wahyudi & Risjani, 2019), inhibitor nitric oxide (NO) (Tanod, Yanuhar, Maftuch, Putra, & Risjani, 2019) dan antibakteri (Tanod, Dewanto, Ndobe, Riyadi & Putra, 2019). Sinularia sp.…”

Section: Pendahuluanmentioning

confidence: 99%

Aktivitas Antioksidan Ekstrak Karang Lunak Asal Teluk Palu, Sulawesi Tengah, Indonesia

Dewanto

Finarti

Hermawan

et al. 2019

JPBKP

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ABSTRAKBioaktif antioksidan merupakan substansi yang penting bagi kesehatan manusia. Karang lunak telah diketahui memproduksi bioaktif dengan keragaman struktur dan aktivitas biologi, termasuk memproduksi bioaktif antioksidan. Tujuan penelitian ini adalah untuk mendapatkan bioprospeksi karang lunak dari perairan Teluk Palu sebagai penghasil antioksidan. Penelitian meliputi pengambilan sampel, identifikasi, ekstraksi (maserasi) karang lunak, skrining konstituen kimia, pengujian aktivitas antioksidan (penangkapan radikal DPPH), dan penentuan IC 50 . Pengambilan sampel dilakukan di Teluk Palu, pesisir desa Kabonga Besar, Kabupaten Donggala, Sulawesi Tengah. Berdasarkan bentuk koloni monomorfik, tujuh sampel karang lunak yang digunakan pada penelitian ini termasuk dalam genus Sinularia, Dampia dan Sarcophyton. Analisis konstituen kimia mengindikasikan adanya senyawa saponin, fenolik, triterpenoid, dan alkaloid. Hasil pengujian aktivitas antioksidan dengan metode DPPH, menunjukkan ekstrak kasar karang lunak menunjukkan persentase inhibisi radikal DPPH yang lemah. Hasil pengujian aktivitas antioksidan (metode DPPH) dari fraksi hasil partisi tujuh ekstrak kasar karang lunak berpotensi sebagai antioksidan dengan kategori kuat sampai lemah. Dari hasil penelitian dapat disimpulkan bahwa ekstrak karang lunak asal Teluk Palu berpotensi sebagai sumber antioksidan. Karang lunak di pesisir Desa Kabonga Besar didominasi oleh genus Sinularia. ABSTRACTThe antioxidants substances are important for human health. Soft corals have been known to produce compounds with a variety of structural and biological activities, including bioactive antioxidants. The aim of this research was to obtain information of soft coral bioprospection from Palu Bay as an antioxidant. The research included sampling, identification, extraction (maceration) of soft corals, screening of chemical constituents, antioxidant activity assay (DPPH's radical scavenger), and determination of IC 50 . Soft coral samples were collected from Palu Bay, coastal village of Kabonga Besar, Donggala District, Central Sulawesi. Based on the shape of the monomorphic colony, seven soft coral samples that were used in this study belong to the genus Sinularia, Dampia and Sarcophyton. Analysis of chemical constituents indicated the presence of saponin compounds, phenolics, triterpenoids, and alkaloids. The results of the antioxidant activity by DPPH method showed that soft coral crude extracts have a weak percentage of DPPH's radical inhibition, while the results of the fraction had potential to be antioxidants with strong to weak categories. From the results of this study, it concluded that the soft coral extracts from Palu Bay have the potential as a source of antioxidants. The genus Sinularia dominates soft coral on the coast of Kabonga Besar Village.

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Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

Cited by 10 publications

References 28 publications

Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

AKTIVITAS ANTIBAKTERI EKSTRAK KARANG LUNAK Sinularia sp. DENGAN METODE BROTH-DILUTION

Aktivitas Antioksidan Ekstrak Karang Lunak Asal Teluk Palu, Sulawesi Tengah, Indonesia

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