Antibacterial potentials of aqueous extract of Enantia chlorantha stem bark

Adesokan, AA; Akanji, Musbau Adewumi; Yakubu, Musa Toyin

doi:10.5897/ajb2007.000-2397

Cited by 54 publications

(12 citation statements)

References 9 publications

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“…Similarly, the methanol leaf extract of A. acuminata and P. granatum had the lowest MIC value, 0.67 mg/ml, and the lowest MBC value 1.51 mg/ml against E. faecalis, K. pneumoniae and P. aeruginosa. The MIC and MBC values against gram-positive bacterium S. aureus and gram-negative bacterium E. coli obtained in this study with the ethanolic, methanolic, and ethyl acetate crude leaf and stem bark extracts of C. macrostachyus were lower than that reported by Minyamer and Belay, [52] and Adesokan et al [53].…”

Section: Discussioncontrasting

confidence: 69%

In-vitro antibacterial activity and phytochemical analysis of leaf and stem bark crude extracts of Croton macrostachyus against selected pathogens, Wolaita Sodo, South Ethiopia

Sivalingam,

Yohanis,

Meseret

et al. 2024

Preprint

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Background: Medicinal plants have long been used as antiseptic, antiallergic, antibacterial, and antiviral medicines to treat human ailments. Croton macrostachyus is one of the important medicinal plants that take an essential part in traditional African medicine, widely used to treat various disorders including diabetes, malaria, and dysentery. Objective: The present research aimed to determine the in-vitro antibacterial activity of ethanol, methanol, and ethyl acetate extracts of C. macrostachyus leaf and stem barks against some selected human bacterial pathogens. Methods: The leaf and stem barks extracts of C. macrostachyus were prepared by by maceration method using three different solvents such as ethanol, methanol, and ethyl acetate. The prepared crude extract was used to determine the antibacterial activity, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) using clinical laboratory standards institute guidelines. Qualitative phytochemical analyses were also performed using the standard protocol. Results: The ethanolic, methanolic, and ethyl acetate crude leaf and stem bark extracts showed inhibitory actions against E. coli, P. aeruginosa and S. aureus. The gram-positive S. aureus was more susceptible to all the crude extracts. The inhibitory activity was concentration-dependent and the leaf crude extracts showed higher activity than stem bark extracts. The methanolic leaf crude extract showed the highest inhibitory activity when compared to ethanol and ethyl acetate. The MIC of methanolic, ethanolic and ethyl acetate crude leaf extracts ranged from 0.625-2.25 mg/ml against selected bacterial pathogens. The MBC results revealed that the methanolic, ethanolic, and ethyl acetate crude leaf extracts showed 1.25-5 mg/ml concentrations against selected bacterial pathogens. The qualitative phytochemical analysis results confirmed the presence of tannins, alkaloids, saponins, flavonoids, glycosides, and phenolic compounds in both ethanolic and methanolic crude leaf extracts and except alkaloids in the ethyl acetate crude leaf extracts. The phytochemical qualitative analysis of stem bark crude extracts results indicated that glycosides were present, alkaloids, flavonoids and phenolic compounds were absent in all three solvent extraction systems, tannins, and saponins were positive with ethanol and ethyl acetate, negative with methanol solvent extractions. Conclusion: The present study concluded that crude leaf extracts showed better antibacterial activity than stem bark extracts against selected pathogens and phytochemical analysis of crude leaf extracts confirmed the presence of phytochemical constituents in the crude extracts prepared from C. macrostachyus and thus, supports the traditional usage of the plant against various diseases.

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Section: Discussioncontrasting

confidence: 69%

In-vitro antibacterial activity and phytochemical analysis of leaf and stem bark crude extracts of Croton macrostachyus against selected pathogens, Wolaita Sodo, South Ethiopia

Sivalingam,

Yohanis,

Meseret

et al. 2024

Preprint

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“…Numerous research groups have also reported such studies throughout the world [. Kharat et al, 2013;Adesokan et al,2007 ]. In recent years ethno medicinal studies received much attention on natural resources to light the numerous medicines, especially of plant origin which needs evaluation on modern scientific lines such as phytochemical analysis, pharmacological and clinical trials.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous research groups have also reported such studies throughout the world [. Kharat et al ., 2013 : Adesokan et al ., 2007 ].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical studies and Antimicrobial Activity on the leaves ofAntigonon leptopusandEcbolium viridae

Rudhra,

Venkatesan

2024

Preprint

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The present study aims to investigate the in vitro Antibacterial activity of Antigonon leptopus and Ecbolium viridae methanol, ethanol, and acetone leaf extracts for against selected bacterial strains respectively. Preliminary phytochemical screening of the leaf crude extract revealed the presence of alkaloids, flavonoids, phenols, tannins, carbohydrates, and saponins. The evaluation of leaf powder was supported by physico-chemical analysis. The antibacterial activities of the leaf of E.viride and Antigonon leptopus in successive different solvents were tested against gram +ve (Staphylococcus aureus, Streptococcus pyogenes) and gram -ve (Escherichia coli, Pseudomonas aeruginosa) Antibacterial activity of those crude extracts are determined by using agar well diffusion method and the zone of inhibition of microorganisms was measured in mm. Amphicillin is used as a positive control respectively. Keywords: Phytochemical screening, Antibacterial activity, Minimum inhibitory concentration (MIC).

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“…The method used by Adesokan et al (2007) was adopted for tube dilution. Thus, the plant extracts were serially diluted from 500 mg/mL solution to obtain varying concentration.…”

Section: Determination Of Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…The plates were incubated at 37 0 C for 24 hours. The least concentration that did not show any growth after incubation was regarded as the MBC (Adesokan et al, 2007).…”

Section: Determination Of Minimum Bactericidal Concentrationmentioning

confidence: 99%

Phytochemical and antibacterial activities of <i>Vitellaria paradoxa</i> and <i>Sclerocarya birrea</i> stem bark and leaves

Sale,

Namadina,

Abubakar

et al. 2023

dujopas

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Increasing resistance developed by many bacterial species has been reported to commonly prescribed antibiotics for bacterial infections. Therefore, the need to search for natural products for remedy of this problem cannot be overemphasized. The aim of the study was to evaluate the phytochemical and antibacterial activities of Vitellaria paradoxa and Sclerocarya birrea ‘stem barks and leaves’ extracts against some human pathogenic bacteria. The methanol and aqueous extracts of Vitellaria paradoxa and Sclerocarya birrea stem barks and leaves were carried out using cold maceration extraction method. Phytochemical screening and acute toxicity studies were carried out using standard methods. Agar well diffusion and agar dilution methods were employed to determine the zone of inhibition, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC). Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, tannins, steroid, carbohydrates and triterpenes in both aqueous and methanolic extracts. All the extracts showed broad spectrum of activity at 500 mg/ml but the methanol extract had larger zones of inhibition and lower M.I.C and M.B.C values ranging from 15.125 mg/ml and 31.25 mg/ml against Staphylococcus aureus and Escherichia coli. The LD50 of Vitellaria paradoxa and Sclerocarya birrea stem barks and leaves were found to be greater than 5000 mg /kg and could be considered safe for consumption. This study has justified the traditional use of Vitellaria paradoxa and Sclerocarya birrea stem barks and leaves extracts in the treatment of infections caused by bacteria. Further investigations should be carried out to isolate pure compounds and determine the mechanisms of action of the plant.

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Antibacterial potentials of aqueous extract of Enantia chlorantha stem bark

Cited by 54 publications

References 9 publications

In-vitro antibacterial activity and phytochemical analysis of leaf and stem bark crude extracts of Croton macrostachyus against selected pathogens, Wolaita Sodo, South Ethiopia

In-vitro antibacterial activity and phytochemical analysis of leaf and stem bark crude extracts of Croton macrostachyus against selected pathogens, Wolaita Sodo, South Ethiopia

Phytochemical studies and Antimicrobial Activity on the leaves ofAntigonon leptopusandEcbolium viridae

Phytochemical and antibacterial activities of <i>Vitellaria paradoxa</i> and <i>Sclerocarya birrea</i> stem bark and leaves

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