Antibacterial properties of bioactive glass particle abraded titanium against Streptococcus mutans

Abstract

The purpose of this study was to evaluate effects of titanium surfaces air-abraded with particles of Bioglass® 45S5 and three-ZnO and SrO doped compositions on the viability, adhesion and biofilm formation of Streptococcus mutans. A statistically significant decrease in the viability of S. mutans was observed for all titanium discs air-particle abraded with the BAGs (p<0.001). Also, a significant effect on diminishing biofilm formation on the titanium discs was seen for all BAGs (p<0.01). No differences were noticed in S. mutans adhesion on titanium surfaces treated with different glasses (p=0.964). Static SBF immersion experiments showed that after 2 and 48 h the BAG doped with 4 mol% ZnO demonstrated the highest Zn 2+ ion concentration released into SBF (0.2 mg L −1 ). 45S5 BAG demonstrated the highest statistically significant increase in the pH throughout the 120 min of static immersion (p<0.001). In conclusion, we showed that titanium alloy discs abraded with particles of the experimental compositions and 45S5 BAG had strong antimicrobial activity against S. mutans and they suppressed S. mutans biofilm formation. The antimicrobial activity of 45S5 BAG was attributed to high pH whereas for the Zn-containing BAGs antimicrobial activity was due to steady release of Zn 2+ into the interfacial solution.

Bioactive Glass Applications in Dentistry

“…BAG-coated dental implants have shown promising results with reduced bone loss in experimentally induced PI in beagle dogs [66]. A recent in vitro study showed reduced biofilm formation of putative periodontal pathogen strains, in addition to S. mutans [67]. Besides, reduced growth of periodontitis-associated and cariogenic bacteria, as well as Enterococcus facials, has been reported using BAG containing propolis, a naturally occurring compound in beehives [68].…”

Effect of bioactive glass air‐abrasion on the wettability and osteoblast proliferation on sandblasted and acid‐etched titanium surfaces

“…Air‐particle abrasion with a bioactive glass has shown an antimicrobial effect and suppressed formation of bacterial biofilm on titanium surfaces in vitro (). The composition of bioactive glass is commonly based on silicon dioxide (SiO 2 )–sodium oxide (Na 2 O)–phosphorus pentoxide (P 2 O 5 )–calcium oxide (CaO) ().…”

“…Several conventional treatment modalities, including curettes, ultrasonic scalers, and air powder abrasion systems, have been used to detoxify and debride contaminated titanium surfaces. However, these conventional approaches are frequently insufficient to lead to the complete removal of bacterial biofilms from rough implant surfaces (8,9) Air-particle abrasion with a bioactive glass has shown an antimicrobial effect and suppressed formation of bacterial biofilm on titanium surfaces in vitro (10). The composition of bioactive glass is commonly based on silicon dioxide (SiO 2 )-sodium oxide (Na 2 O)-phosphorus pentoxide (P 2 O 5 )-calcium oxide (CaO) (11).…”

Effect of bioactive glass air‐abrasion on Fusobacterium nucleatum and Porphyromonas gingivalis biofilm formed on moderately rough titanium surface

“…As the glass dissolves, these metal ions are released into the interfacial solution at concentrations that induce the desired antibacterial effect. Zinc (Zn) ions are one of the most potent antibacterial elements that inhibit dental plaque pathogens, including Streptococcus mutans, F. nucleatum, and P. gingivalis [27][28][29]. Moreover, the addition of Zn oxide has been found to accelerate bone formation and to enhance BG mechanical properties [30].…”