Antibacterial properties of glycosylated surfaces: variation of the glucosidal moiety and fatty acid conformation of grafted microbial glycolipids

Valotteau, Claire; Roelants, Sophie; Dasaiyan, Prabhu; Zibek, Susanne; Günther, Michael; Soetaert, Wim; Everaert, Bernd; Pradier, Claire‐Marie; Babonneau, Florence; Baccile, Niki; Humblot, Vincent

doi:10.1039/d0me00059k

Cited by 8 publications

(7 citation statements)

References 69 publications

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“…Microbial biosurfactants are biobased compounds and have attracted the interest of the medical industry for the development of new medicines due to their antimicrobial properties (Valotteau et al 2020 ). MELs also exhibit antimicrobial properties due to their damaging effect on the bilayer cell membranes of microorganisms (Fukuoka et al 2007 ).…”

Section: Resultsmentioning

confidence: 99%

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Efficient production of mannosylerythritol lipids by a marine yeast Moesziomyces aphidis XM01 and their application as self-assembly nanomicelles

Wang

Zhang

et al. 2022

Mar Life Sci Technol

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Mannosylerythritol lipids (MELs) are one of the most promising biosurfactants because of their excellent physicochemical properties, high environmental compatibility, and various biological functions. In this study, a mangrove yeast strain Moesziomyces aphidis XM01 was identified and used for efficient extracellular MEL production. The MEL titer reached 64.5 ± 0.7 g/L at flask level within 7 days with the optimized nitrogen and carbon source of 2.0 g/L NaNO3 and 70 g/L soybean oil. Furthermore, during a 10-L two-stage fed-batch fermentation, the final MEL titer reached 113.6 ± 3.1 g/L within 8 days, with prominent productivity and yield of 14.2 g·L−1·day−1 and 94.6 g/g(glucose and soybean oil). Structural analysis indicated that the produced MELs were mainly MEL-A and its fatty acid profile was composed of only medium-chain fatty acids (C8–C12), especially C10 acids (77.81%). Further applications of this compound were evaluated as one-step self-assembly nanomicelles. The obtained MEL nanomicelles showed good physicochemical stability and antibacterial activity. In addition, using clarithromycin as a model hydrophobic drug, the MEL nanomicelles exhibited high loading capacity and could be used for the controlled and sustained drug release in low-pH environments. Therefore, M. aphidis XM01 is an excellent candidate for efficient MEL production, and the prepared MEL nanomicelles have broad application prospects in the pharmaceutical and cosmetic fields.

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Section: Resultsmentioning

confidence: 99%

“…Glycolipids can be used as biosurfactants with unique properties compared to chemical counterparts, such as high environmental compatibility, mild production conditions, and various biological functions (Coelho et al 2020 ; Valotteau et al 2020 ). In this regard, MELs are one of the most promising glycolipids.…”

Section: Introductionmentioning

confidence: 99%

Efficient production of mannosylerythritol lipids by a marine yeast Moesziomyces aphidis XM01 and their application as self-assembly nanomicelles

Wang

Zhang

et al. 2022

Mar Life Sci Technol

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“…LIVE/DEAD imaging uses a staining technique that fluoresces damaged and intact cells differently when fluorescence microscopy is performed. These images can be subjected to cell-counting software that determine the ratio of living to apoptotic cells across the surface sample [92]. Similarly, bacteria can be tagged with fluorochrome solutions that create visible membrane stains under fluorescence microscopy.…”

Section: Characterization Methods For Biofilmsmentioning

confidence: 99%

Functionalized Self-Assembled Monolayers: Versatile Strategies to Combat Bacterial Biofilm Formation

et al. 2022

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Bacterial infections due to biofilms account for up to 80% of bacterial infections in humans. With the increased use of antibiotic treatments, indwelling medical devices, disinfectants, and longer hospital stays, antibiotic resistant infections are sharply increasing. Annual deaths are predicted to outpace cancer and diabetes combined by 2050. In the past two decades, both chemical and physical strategies have arisen to combat biofilm formation on surfaces. One such promising chemical strategy is the formation of a self-assembled monolayer (SAM), due to its small layer thickness, strong covalent bonds, typically facile synthesis, and versatility. With the goal of combating biofilm formation, the SAM could be used to tether an antibacterial agent such as a small-molecule antibiotic, nanoparticle, peptide, or polymer to the surface, and limit the agent’s release into its environment. This review focuses on the use of SAMs to inhibit biofilm formation, both on their own and by covalent grafting of a biocidal agent, with the potential to be used in indwelling medical devices. We conclude with our perspectives on ongoing challenges and future directions for this field.

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“…The number of glucose units and the linker backbone have an impact on the biocidal properties of the surface. 20 The study reported here aimed at imparting specific recognizing function and enhancing the antimicrobial features of ESM. The design of an active layer with good mechanical stability is a key prerequisite for a material with targeted application such as sensing, filtering or healthcare devices.…”

Section: Introductionmentioning

confidence: 99%

“…It has been also shown that glycosylated surfaces can display antimicrobial properties. The number of glucose units and the linker backbone have an impact on the biocidal properties of the surface 20 …”

Section: Introductionmentioning

confidence: 99%

Modification of eggshell membrane to impart biospecific properties

Todorova

Nikolova

Popova

et al. 2021

Polymer International

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Eggshell membrane (ESM) represents an inexpensive, environmentally friendly and nontoxic byproduct. Besides the unique mechanical properties of the material, the ESM surface is rich in functional groups susceptible to further modifications. In the current study two modification pathways were applied to enrich the ESM surface with glucopyranosyl units. One was based on the reaction of activated surface carboxylic groups with glucosamine and the other exploited the reactivity of the surface amino groups for grafting poly(ethylene glycol) (PEG) chains bearing end glucopyranosyl and benzaldehyde moieties. The functionalization of the PEG chains with molar masses 400 and 1500 g mol−1 was evidenced from 1H NMR and 13C NMR spectral data. The modified ESMs were characterized using Fourier transform infrared spectroscopy and scanning electron microscopy. The Brunauer–Emmett–Teller specific surface area and the total pore volume of ESM decreased upon modification. Decoration of ESM with glucopyranosyl units imparted protein recognition properties that were tested via binding studies with concanavalin A as model lectin. Further, grafting of glucose units via PEG linkers reduced bacterial adhesion onto the membrane surface. © 2021 Society of Industrial Chemistry.

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Antibacterial properties of glycosylated surfaces: variation of the glucosidal moiety and fatty acid conformation of grafted microbial glycolipids

Abstract: Glycosylated surfaces can display antimicrobial properties.

Cited by 8 publications

References 69 publications

Efficient production of mannosylerythritol lipids by a marine yeast Moesziomyces aphidis XM01 and their application as self-assembly nanomicelles

Efficient production of mannosylerythritol lipids by a marine yeast Moesziomyces aphidis XM01 and their application as self-assembly nanomicelles

Functionalized Self-Assembled Monolayers: Versatile Strategies to Combat Bacterial Biofilm Formation

Modification of eggshell membrane to impart biospecific properties

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