Antibacterial resistance of Vibrio parahaemolyticus isolated from shrimp farms located in east coastal region of the Mekong Delta, Vietnam

Abstract

The study was conducted to evaluate the susceptibility of 58 Vibrio parahaemolyticus bacterial isolates to 16 antibiotics. These bacterial isolates were recovered from diseased shrimp which displayed typical pathology of AHPND such as hepatopancreatic atrophy, empty gut, and hepatopancreatic changes including hemocytic infiltration and bacterial infection. Results of antibiotic susceptibility testing by the disk diffusion method showed that single resistance to tested antibiotics was relatively rare. The bacterial strains were resistant to amoxicillin (100%), cephalexin (100%), Sulfadiazine Sodium (94.7%), and Erythromycin (87.7%). Sensitivity of tested strains was recorded with doxycycline (84.2%) and oxytetacylin (49%). The minimum inhibitory concentrations (MICs) were determined for the sensitive isolates using a broth macro dilution method. The majority of tested isolates had an MIC value of 2μg/mL with doxycycline. The current study suggests caution in the use of antibiotics for the prevention and treatment of AHPND in shrimp farming.

Paclobutrazol Biodegradation of Native Bacteria in Mango Orchards in Mekong Delta, Vietnam

“…The identification of the four strains based on sequencing of the 16S rDNA using 27F and 1495R primer pair showed that the bacterial strains NL-P5, NL-P7, NL-P12 and NL-P17 related to Acinetobacter seifertii, Pseudomonas nitroreducens, Burkholderia cepacia and Pseudomonas fluorescens, respectively. This result was consistent with the study of Chen et al (2010), Santos et al (2014), Dang et al (2014) and Vaz et al (2015).…”

“…isolated from industrially contaminated soil in China was able to biodegrade up to 60% of PBZ at an initial concentration of 54 ppm in 48 hours and 98% of PBZ at a concentration of 3.4 ppm in 36 hours. According to research by Dang et al (2014), Burkherderia sp. was isolated on mango soil in Mekong Delta, Vietnam showed the ability to biodegrade 15.53% to 16.41% of the initial 15 ppm PBZ concentration after 15 days of culture.…”

Meta-Analysis and Systematic Review of Phenotypic and Genotypic Antimicrobial Resistance and Virulence Factors in Vibrio parahaemolyticus Isolated from Shrimp

“…Figure 2.The meta-analysis of prevalence of V. parahaemolyticus and virulence factors in shrimp using random-effects model with a 95% C.I. A forest plot of (A) prevalence of V. parahaemolyticus[19,[25][26][27][28][29]31,[33][34][35][36][37][38][39][40][41][42][43][44][46][47][48][49]51,53] and (B) prevalence of virulence factors[4,24,28,33-36,38- 40,42,44,45,47,49,51-53] representing the results in meta-analysis with size squares proportional to the weight assigned to the study; a funnel plot of the prevalence estimates of V. parahaemolyticus (C) and virulence factors (D) to assess potential bias. A red vertical line represents the overall prevalence from meta-analysis, and the diagonal lines provide 95% C.I.…”