Antibacterial <scp>graphene‐based</scp> hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

Stevanović, Milena; Djošić, Marija; Janković, Ana; Kojić, Vesna; Vukašinović-Sekulić, Maja; Stojanović, Jovica; Odović, Jadranka; Sakač, Milkica Crevar; Yop, Rhee Kyong; Mišković‐Stanković, Vesna

doi:10.1002/jbm.a.36974

Cited by 49 publications

(30 citation statements)

References 84 publications

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Section: Antibacterial Evaluationssupporting

confidence: 93%

“…We observed that the zones were the same after 5 days of incubation and 11 days of incubation. These results are in line with previous studies on antibiotic coatings against S. aureus and other bacteria [17,18,81]. When examining the antibacterial effect by means of the broth dilution method, the PEEK-HAP1-Cef sample possessed a higher antibacterial effect against S. aureus compared to PEEK-HAP1 and the control sample (PEEK only; Figure 9A).…”

Section: Antibacterial Evaluationssupporting

confidence: 91%

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Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method

et al. 2021

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Medical implant use is associated with a risk of infection caused by bacteria on their surface. Implants with a surface that has both bone growth-promoting properties and antibacterial properties are of interest in orthopedics. In the current study, we fabricated a bioactive coating of hydroxyapatite nanoparticles on polyether ether ketone (PEEK) using the sonocoating method. The sonocoating method creates a layer by immersing the object in a suspension of nanoparticles in water and applying a high-power ultrasound. We show that the simple layer fabrication method results in a well-adhering layer with a thickness of 219 nm to 764 nm. Dropping cefuroxime sodium salt (Cef) antibiotic on the coated substrate creates a layer with a drug release effect and antibacterial activity against Staphylococcus aureus. We achieved a concentration of up to 1 mg of drug per cm2 of the coated substrate. In drug release tests, an initial burst was observed within 24 h, accompanied by a linear stable release effect. The drug-loaded implants exhibited sufficient activity against S. aureus for 24 and 168 h. Thus, the simple method we present here produces a biocompatible coating that can be soaked with antibiotics for antibacterial properties and can be used for a range of medical implants.

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Section: Antibacterial Evaluationssupporting

confidence: 93%

Section: Antibacterial Evaluationssupporting

confidence: 91%

Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method

et al. 2021

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“…Above everything mentioned before, chitosan has very high position in the field of biomaterials production due to its exceptional properties of easy film forming ability [ 57 , 70 ]. Chitosan, as a cationic polysaccharide, can be used in the electrophoretic deposition process (EPD), one of the most attractive techniques for bioactive coatings production [ 68 , 69 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ]. Lignin (Lig) is a polyphenolic polymer, originated from the various natural sources [ 78 , 79 , 80 ].…”

Section: Engineering Implant Surfaces To Prevent Microbial Adhesion and Infectionmentioning

confidence: 99%

“…Composite coatings on Ti were electrophoretically deposited from two different aqueous suspensions, HAP/CS/Gent (1 wt.% of HAP, 0.05 wt.% of CS and 0.1 wt.% of Gent) and HAP/CS/Gr/Gent (1 wt.% of HAP, 0.05 wt.% of CS, 0.01 wt.% of Gr, and 0.1 wt.% of Gent). A three-electrode cell arrangement, e.g., titanium plate as a working electrode and two Pt panels as counter electrodes were employed to deposit composite coatings at room temperature, constant voltage of 5 V and deposition time of 12 min [ 68 , 71 , 72 ].…”

Section: Designing Coatings With Long-term Release Of Antibioticsmentioning

confidence: 99%

Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium

2021

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Current trends in biomaterials science address the issue of integrating artificial materials as orthopedic or dental implants with biological materials, e.g., patients’ bone tissue. Problems arise due to the simple fact that any surface that promotes biointegration and facilitates osteointegration may also provide a good platform for the rapid growth of bacterial colonies. Infected implant surfaces easily lead to biofilm formation that poses a major healthcare concern since it could have destructive effects and ultimately endanger the patients’ life. As of late, research has centered on designing coatings that would eliminate possible infection but neglected to aid bone mineralization. Other strategies yielded surfaces that could promote osseointegration but failed to prevent microbial susceptibility. Needless to say, in order to assure prolonged implant functionality, both coating functions are indispensable and should be addressed simultaneously. This review summarizes progress in designing multifunctional implant coatings that serve as carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the implant-tissue interface, while still promoting osseointegration.

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“…These include the presence of amorphous phases resulting from the extremely high process temperatures [26] and the difficulty of coating implants with complex geometrical features due to the line-of-sight nature of the process [27]. Alternative methods include: Sol-gel processing [28,29], biomimetic deposition [30][31][32], hydrothermal processing [33], electrophoresis [34], gas detonation deposition [35][36][37], and electrodeposition [38][39][40]. Among these, electrodeposition is the most promising technique because it can deposit homogeneous HAp coatings on interior and exterior surfaces of metallic scaffolds, regardless of a sample's size or pore geometry.…”

Section: Introductionmentioning

confidence: 99%

Electrodeposition of Hydroxyapatite on a Metallic 3D-Woven Bioscaffold

et al. 2020

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In this study, we demonstrate that a uniform coating of hydroxyapatite (HAp, Ca10(PO4)6(OH)2) can be electrochemically deposited onto metallic 3D-woven bone scaffolds to enhance their bioactivity. The HAp coatings were deposited onto metallic scaffolds using an electrolyte containing Ca(NO3)2·4H2O, NH4H2PO4, and NaNO3. The deposition potential was varied to maximize the uniformity and adhesion of the coating. Using X-ray diffraction (XRD), Raman spectroscopy, and energy-dispersive spectroscopy (EDS), we found crystallized HAp on the 3D-woven lattice under all deposition potentials, while the −1.5 V mercury sulfate reference electrode potential provided the best local uniformity with a satisfactory deposition rate. The coatings generated under this optimized condition were approximately 5 µm thick and uniform throughout the internal and external sections of the woven lattice. We seeded and cultured both coated and uncoated scaffolds with human adipose-derived stromal/stem cells (ASCs) for 12 h and 4 days. We observed that the HAp coating increased the initial cell seeding efficiency by approximately 20%. Furthermore, after 4 days of culture, ASCs cultured on HAp-coated stainless-steel scaffolds increased by 32% compared to only 17% on the uncoated scaffold. Together, these results suggest that the HAp coating improves cellular adhesion.

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Antibacterial graphene‐based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

Cited by 49 publications

References 84 publications

Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method

Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method

Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium

Electrodeposition of Hydroxyapatite on a Metallic 3D-Woven Bioscaffold

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