Antibiofilm action of Persea americana glycolic extract over Acinetobacter baumannii and absence of toxicity in Galleria mellonella

Abstract

Objectives This study aimed to evaluate the antibiofilm activity and toxicity of the glycolic extract of Persea americana “P. americana” over multidrug-resistant strains of Acinetobacter baumannii “A. baumannii” as alternative therapy to be investigated. Methods A bacterial inoculum of each bacterial strain (4a, 5a, 9a, 12a, ATCC 19606) of A. baumannii was prepared and adjusted by the spectrophotometer. The microdilution broth method was performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). P. americana glycolic extracts were obtained of the tree stalk and leaves. The biofilm viability was tested by MTT assay after 5 min exposure. The toxicity of the extracts was tested by invertebrate model Galleria mellonella. The data were analyzed by ANOVA, Tukey test and log-rank method (α=0.05). Results The extract showed an inhibitory and bactericidal action over all the tested strains with the lowest MIC value observed for the reference strain (3.12 mg/mL). The extract did not demonstrate toxicity in any of the tested concentrations (12.5, 25 and 50 mg/mL) in Galleria mellonella larvae, with a survival percentage above 80% after 168 h. Conclusions The glycolic extract of P. americana has microbicidal and antibiofilm activity on multidrug-resistant clinical strains of A. baumannii and showed low toxicity for the invertebrate model G. mellonella.

Antimicrobial Action, Genotoxicity, and Morphological Analysis of Three Calcium Silicate-Based Cements

“…Finally, the CSCs have a finality of promoting tissue repair in interesting areas for apexogensis, apexification, and apicectomy [ 3 – 5 , 35 ] or sealing of perforated area [ 2 ] and some advantages in vital pulp therapy approaches [ 36 ]. Thus, the most expected of these material are not to be genotoxic, and has antimicrobial action, or to not favor the contamination; still, this action may be improved by the use of antimicrobial agents like sodium hypochlorite and others [ 24 , 37 , 38 ] and by complementary technique like photodynamic therapy and passive ultrasonic irrigation [ 21 , 23 , 25 , 37 ].…”

The Effect of Aloe vera and Chlorhexidine as Disinfectants on the Success of Selective Caries Removal Technique: A Randomized Controlled Trial

“…Phytotherapy is gaining a greater space in dentistry because of the antimicrobial effect, anti-inflammatory action, and biocompatibility of diverse herbal medicines [ 11 , 16 , 18 ]. Aloe vera extract as an herbal medicine was tested in this study to evaluate its effect on the success of the selective caries removal technique of deep carious lesions combined with asymptomatic reversible pulpitis when used as a cavity disinfectant.…”

“…Besides, Aloe vera , an herbal medicine, belongs to the Liliaceae family [ 9 ]. Like other herbal extracts [ 10 , 11 ], it was indicated as an oral antiseptic for the prevention of dental caries and periodontal diseases [ 12 ] because of its antimicrobial effect, anti-inflammatory action [ 13 , 14 ], and bone-dentin bridge formation inducing property [ 15 , 16 ].…”

Antimicrobial and Antibiofilm Effect of Brazilian Green Propolis Aqueous Extract against Dental Anaerobic Bacteria

“…Phytotherapy is making great efforts in this aspect, in which diverse medicinal plants were presented recently as alternative antimicrobials to treat diverse infections because of their antimicrobial action and biocompatibility [ 16 , 17 , 18 , 19 ]. Propolis is a resinous substance enriched by biologically active molecules and is produced by Apis mellifera through the collection of different plant parts [ 20 ].…”