Antibiofilm activity of cashew juice pulp against Staphylococcus aureus , high performance liquid chromatography/diode array detection and gas chromatography-mass spectrometry analyses, and interference on antimicrobial drugs

Abstract

The epidemiology of Staphylococcus aureus infections has evolved in recent years, as this species is a major Gram-positive pathogen associated with healthcare services. The antimicrobial resistance of this species raises an urgent need for new treatment strategies. Fruits play important nutritional and economic roles in society, but their biological and pharmacological features are poorly explored when compared to nonedible parts of plants such as barks and leaves. In this study, we show that the cashew apple juice [cashew juice pulp (CJP)] extract is active against the planktonic cells of S. aureus strains, and for the first time, we show that CJP is also active against S. aureus biofilms. High performance liquid chromatography and gas chromatography-mass spectrometry analyses were conducted to prospect for polyphenols and free carbohydrates, respectively. Cashew apple juice, which is rich in nutrients, is widely consumed in Brazil; therefore, the quality attributes of CJPs were investigated. Samples were evaluated for pH, total titratable acidity, vitamin C levels, and total soluble solids. We also detected an antagonistic interference of CJP when it was combined with different antimicrobial drugs.

Dexamethasone abrogates the antimicrobial and antibiofilm activities of different drugs against clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa

“…Although clinical isolates were used in this study, our sample is limited to 10 strains of each species; thus, researches with larger samples are important to confirm our observations [40]. Despite the antimicrobials used in this study are highly relevant in clinical treatments of infectious diseases, it would be of interest to conduct the assays with a larger number of drugs from different pharmacological groups, in order to explore the combined used of DEXA with drugs of distinct mechanisms of action.…”

Evaluation of Antioxidant and Antibacterial Activities of Fresh and Freeze-Dried Selected Fruit Juices

“…This may be due to the chemical changes. The previous study demonstrated that the presence of polyphenols may have contributed to this result, as many molecules of this chemical group can appear antimicrobial activity [27]. In the investigation of the FDS of C. carandas demonstrated the antimicrobial activity against the 10 tested microorganisms, showed that C. carandas was the potential antibacterial agent.…”

Profile of phenolic compounds and antimicrobial potential of hydroalcoholic extracts from cashew-apple coproducts

“…For CBE-F, a higher concentration was required to obtain a complete inhibition of the L. monocytogenes and S. aureus bacteria, with MIC values of 8.300 µg mL -1 (Table 6). Dias-Souza et al (2017) reported that the extract of pulp cashew presented inhibitory and bactericidal effects against Staphylococcus aureus strains at 15.60 and 125 µg mL -1 respectively; and HPLC analysis confirmed the presence of polyphenols associated with antimicrobial activity.…”