Antibiofilm activity of nanosilver coatings against Staphylococcus aureus

Geissel, Felix J.; Platania, Varvara; Gogos, Alexander; Herrmann, Inge K.; Belibasakis, Georgios N.; Chatzinikolaidou, Μaria; Sotiriou, Georgios A.

doi:10.1016/j.jcis.2021.11.038

Cited by 39 publications

(31 citation statements)

References 40 publications

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“…The strong antimicrobial power of nanosilver and the bacteriostatic action of compounds such as chitosan and tannic acid provide the synergy required to prevent resistance development. Notably, Ag-based compositions have proven to be effective against biofilm development, exhibiting an inhibitory effect on mature monomicrobial biofilms [ 36 , 37 , 38 ]. The combination of nanosilver with organic antimicrobials result in not only antibacterial and antibiofilm action but also antiviral potential.…”

Section: Introductionmentioning

confidence: 99%

Highly Stable Core-Shell Nanocolloids: Synergy between Nano-Silver and Natural Polymers to Prevent Biofilm Formation

et al. 2022

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Active investment in research time in the development and study of novel unconventional antimicrobials is trending for several reasons. First, it is one of the ways which might help to fight antimicrobial resistance and bacterial contamination due to uncontrolled biofilm growth. Second, minimizing harmful environmental outcomes due to the overuse of toxic chemicals is one of the highest priorities nowadays. We propose the application of two common natural compounds, chitosan and tannic acid, for the creation of a highly crosslinked polymer blend with not only intrinsic antimicrobial properties but also reducing and stabilizing powers. Thus, the fast and green synthesis of fine spherically shaped Ag nanoparticles and further study of the composition and properties of the colloids took place. A positively charged core-shell nanocomposition, with an average size in terms of the metal core of 17 ± 4 nm, was developed. Nanoantimicrobials were characterized by several spectroscopic (UV-vis and FTIR) and microscopic (transmission and scanning electron microscopies) techniques. The use of AgNPs as a core and an organic polymer blend as a shell potentially enable a synergistic long-lasting antipathogen effect. The antibiofilm potential was studied against the food-borne pathogens Salmonella enterica and Listeria monocytogenes. The antibiofilm protocol efficiency was evaluated by performing crystal violet assay and optical density measurements, direct visualization by confocal laser scanning microscopy and morphological studies by SEM. It was found that the complex nanocomposite has the ability to prevent the growth of biofilm. Further investigation for the potential application of this stable composition in food packaging will be carried out.

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Section: Introductionmentioning

confidence: 99%

Highly Stable Core-Shell Nanocolloids: Synergy between Nano-Silver and Natural Polymers to Prevent Biofilm Formation

et al. 2022

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“…Previously, several peptide classes of antibiotics like vancomycin, nisin A, rifampicin, quinolones with nitroxides combination with several anticancer drugs on a repurposing basis showed effectiveness against biofilm‐producing pathogens [54,59,64–66] . Natural products and anti‐infective agents bearing nano‐coatings (nanomaterials) are also commonly used to protect against biofilms on medical devices/instruments, surgical/wound sites [67,68] . Bioinformatics tools can mimic biological systems through programming and coding; which plays a crucial role in the current drug development process for selecting the most druggable candidates before conducting an experiment.…”

Section: Resultsmentioning

confidence: 99%

“…[54,59,[64][65][66] Natural products and anti-infec-tive agents bearing nano-coatings (nanomaterials) are also commonly used to protect against biofilms on medical devices/instruments, surgical/wound sites. [67,68] Bioinformatics tools can mimic biological systems through programming and coding; which plays a crucial role in the current drug development process for selecting the most druggable candidates before conducting an experiment. Here, the selection of 30 insect-derived antibiofilm peptides out of 133 is an example.…”

Section: Homologues-cum-phylogenetic Tree Analysis Of Peptidesmentioning

confidence: 99%

In Silico Identification of Potential Insect Peptides against Biofilm‐Producing Staphylococcus aureus

Sahoo

Swain

Panda

et al. 2022

Chemistry & Biodiversity

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Biofilm-producing Staphylococcus aureus (SA) strains are frequently found in medical environments, from surgical/ wound sites, medical devices. These biofilms reduce the efficacy of applied antibiotics during the treatment of several infections, such as cystic fibrosis, endocarditis, or urinary tract infections. Thus, the development of potential therapeutic agents to destroy the extra protective biofilm layers or to inhibit the biofilm-producing enzymes is urgently needed. Advanced and cost-effective bioinformatics tools are advantageous in locating and speeding up the selection of antibiofilm candidates. Based on the potential drug characteristics, we have selected one-hundred thirty-three antibacterial peptides derived from insects to assess for their antibiofilm potency via molecular docking against five putative biofilm formation and regulated target enzymes: the staphylococcal accessory regulator A or SarA (PDB ID: 2FRH), 4,4'-diapophytoene synthase or CrtM (PDB ID: 2ZCQ), clumping factor A or ClfA (PDB ID: 1N67) and serine-aspartate repeat protein C or SdrC (PDB ID: 6LXH) and sortase A or SrtA (PDB ID: 1T2W) of SA bacterium. In this study, molecular docking was performed using HPEPDOCK and HDOCK servers, and molecular interactions were examined using BIOVIA Discovery Studio Visualizer-2019. The docking score (kcal/mol) range of five promising antibiofilm peptides against five targets was recorded as follows: diptericin A (À 215.52 to À 303.31), defensin (À 201.11 to À 301.92), imcroporin (À 212.08 to À 287.64), mucroporin (À 228.72 to À 286.76), apidaecin II (À 203.90 to À 280.20). Among these five, imcroporin and mucroporin were 13 % each, while defensin contained only 1 % of positive net charged residues (Arg + Lys) projected through ProtParam and NetWheels tools. Similarly, imcroporin, mucroporin and apidaecin II were 50 %, while defensin carried 21.05 % of hydrophobic residues predicted by the tool PEPTIDE. 2.0. Most of the peptides exhibited potential characteristics to inhibit S. aureus-biofilm formation via disrupting the cell membrane and cytoplasmic integrity. In summary, the proposed hypothesis can be considered a cost-effective platform for selecting the most promising bioactive drug candidates within a limited timeframe with a greater chance of success in experimental and clinical studies.

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“…Silver (Ag) and gold (Au)-NPs might be manufactured using naturally occurring reducing materials from different sources such as plants, fungus, honey, chitosan, bacteria, and yeast [ 4 ]. Silver nanoparticles have been studied as antibiofilm materials in several studies due to their potent antibacterial activities [ 5 ]. Goderecci et al [ 6 ] evaluated the antibacterial activity of nanosilver oxide (AgO) films against Staphylococcus aureus and Escherichia coli after being deposited on titanium foil discs.…”

Section: Introductionmentioning

confidence: 99%

Molasses-Silver Nanoparticles: Synthesis, Optimization, Characterization, and Antibiofilm Activity

Dorgham

Moaty

Chong

et al. 2022

IJMS

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Biofilms are matrix-enclosed communities of bacteria that are highly resistant to antibiotics. Adding nanomaterials with antibacterial activity to the implant surfaces may be a great solution against biofilm formation. Due to its potent and widespread antibacterial effect, silver nanoparticles were considered the most potent agent with different biological activities. In the present investigation, silver nanoparticles (AgNPs) were newly synthesized as antibiofilm agents using sugarcane process byproduct (molasses) and named Mo-capped AgNPs. The synthesized nanoparticles showed promising antimicrobial activity against S. aureus ATCC 6538 and C. albicans DAY185. Statistically designed optimization through response surface methodology was evaluated for maximum activity and better physical characteristics, namely the nanoparticles’ size and polydispersity index (PDI), and it was revealed that molasses concentration was the main effective factor. Minimal biofilm eradication concentration (MBEC) of Mo-capped AgNPs against S. aureus ATCC 6538 and C. albicans DAY185 was 16 and 32 µg/mL, respectively. Scanning electron microscope study of Mo-capped AgNP-treated biofilm revealed that AgNPs penetrated the preformed biofilm and eradicated the microbial cells. The optimally synthesized Mo-capped AgNPs were spherically shaped, and the average size diameter ranged between 29 and 88 nm with high proportions of Ag+ element (78.0%) recorded. Fourier-transform infrared spectroscopy (FTIR) analysis indicated the importance of molasses ingredients in capping and stabilizing the produced silver nanoparticles.

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Antibiofilm activity of nanosilver coatings against Staphylococcus aureus

Cited by 39 publications

References 40 publications

Highly Stable Core-Shell Nanocolloids: Synergy between Nano-Silver and Natural Polymers to Prevent Biofilm Formation

Highly Stable Core-Shell Nanocolloids: Synergy between Nano-Silver and Natural Polymers to Prevent Biofilm Formation

In Silico Identification of Potential Insect Peptides against Biofilm‐Producing Staphylococcus aureus

Molasses-Silver Nanoparticles: Synthesis, Optimization, Characterization, and Antibiofilm Activity

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