Antibiofilm and Anti-��-Lactamase Activities of Burdock Root Extract and Chlorogenic Acid against Klebsiella pneumoniae

Rajasekharan, Satish Kumar; Ramesh, S.; Satish, Ann Susan; Lee, Jintae

doi:10.4014/jmb.1609.09043

Cited by 50 publications

(25 citation statements)

References 37 publications

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“…Staphylococcus aureus biofilms are important in chronic and persistent mastitis infections and can cause bacterial resistance to treatment with antimicrobial agents (Melchior et al, 2006). A previous study demonstrated that CGA could significantly repress biofilm formation in the context of persistent Klebsiella pneumoniae infection (Rajasekharan et al, 2017). Although statistical significance was not reached, our results suggest a trend for the reduction of biofilm with CGA treatment in BMEC cultures challenged with S. aureus.…”

Section: Discussioncontrasting

confidence: 62%

The protective effect of chlorogenic acid on bovine mammary epithelial cells and neutrophil function

Gong

Zhan

et al. 2018

Journal of Dairy Science

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Chlorogenic acid (CGA) is the ester of caffeic acid and quinic acid and plays an important role in antibacterial activity and anti-inflammatory properties. The objective of this study was to examine the effects of CGA on the growth of Staphylococcus aureus and the mRNA levels of the genes encoding the inflammatory response cytokines, κ-casein, and neutrophil function in bovine mammary epithelial cells (BMEC) exposed to S. aureus. Chlorogenic acid has important antibacterial, antioxidant, and anti-inflammatory functions; however, the effect of CGA on BMEC and neutrophils exposed to S. aureus has not been investigated previously. Our results demonstrated that 10, 20, and 30 μg/mL CGA had no cytotoxic effects on BMEC in culture, and that 20 μg/mL CGA enhanced the viability of BMEC exposed to S. aureus, whereas 30 μg/mL CGA reduced S. aureus growth after 9 h compared with controls. The rate of S. aureus invasion into BMEC was also attenuated by 30 μg/mL CGA compared with controls, whereas this treatment led to reduced abundance of IL6, IL8, and TLR2 mRNA in S. aureus-exposed BMEC. Migration of bovine polymorphonuclear leukocytes was significantly decreased in S. aureus-exposed BMEC with 10 and 20 μg/mL CGA treatment when compared with S. aureus treatment alone. In addition, incubation with 20 or 30 μg/mL CGA enhanced the phagocytic ability of polymorphonuclear leukocytes compared with the control group. Importantly, levels of κ-casein were enhanced by treatment of S. aureus-exposed BMEC with CGA. Our results suggest that the use of CGA may be a potent therapeutic tool against bovine mastitis caused by S. aureus.

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Section: Discussioncontrasting

confidence: 62%

The protective effect of chlorogenic acid on bovine mammary epithelial cells and neutrophil function

Gong

Zhan

et al. 2018

Journal of Dairy Science

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“…Again, the plates were incubated statically under the same conditions of time and temperature. Then, the same process described above was performed before evaluation with a microplate spectrophotometer [ 46 ]. Statistical significance was determined by pair-wise testing using the Student’s t-test, and the results were considered statistically significant when p < 0.05 for the rejection of the null hypothesis.…”

Section: Methodsmentioning

confidence: 99%

Terpinen-4-ol as an Antibacterial and Antibiofilm Agent against Staphylococcus aureus

Cordeiro

Figueiredo

Souza

et al. 2020

IJMS

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Staphylococcus aureus is able to rapidly develop mechanisms of resistance to various drugs and to form strong biofilms, which makes it necessary to develop new antibacterial drugs. The essential oil of Melaleuca alternifolia is used as an antibacterial, a property believed to be mainly due to the presence of terpinen-4-ol. Based on this, the objective of this study was to evaluate the antibacterial and antibiofilm potential of terpinen-4-ol against S. aureus. The Minimal Inhibitory and Minimal Bactericidal Concentrations (MIC and MBC) of terpinen-4-ol were determined, and the effect of its combination with antibacterial drugs as well as its activity against S. aureus biofilms were evaluated. In addition, an in silico analysis of its pharmacokinetic parameters and a molecular docking analysis were performed. Terpinen-4-ol presented a MIC of 0.25% (v/v) and an MBC of 0.5% (v/v) (bactericidal action); its association with antibacterials was also effective. Terpinen-4-ol has good antibiofilm activity, and the in silico results indicated adequate absorption and distribution of the molecule in vivo. Molecular docking indicated that penicillin-binding protein 2a is a possible target of terpinen-4-ol in S. aureus. This work highlights the good potential of terpinen-4-ol as an antibacterial product and provides support for future pharmacological studies of this molecule, aiming at its therapeutic application.

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“…Chlorogenic acid (CA), one of the most common polyphenol compounds, is abundant in coffee beans, and also exist in various vegetables and fruits . Studies have revealed that CA has pharmacological activities against bacteria, inflammation and oxidation, as well as hyperlipidemia disease . In addition, several studies showed that CA shows anticancer properties.…”

Section: Introductionmentioning

confidence: 99%

Chlorogenic acid inhibits proliferation and induces apoptosis in A498 human kidney cancer cells via inactivating PI3K/Akt/mTOR signalling pathway

Wang

Liu

Xie

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives Kidney cancer is a highly lethal cancer, of which the most common type is renal cell carcinoma (RCC). The targeted drugs used in treating RCC clinically have a lot of side effects. Therefore, it is urgent to find out effective agents with little toxic effects. Methods The antiproliferation effect of chlorogenic acid (CA) was performed using the CCK-8 assay. Then, we adopted colony formation assay, Annexin V/PI staining assay and JC-1 mitochondrial membrane potential assay to explore the mechanism of anticancer effect of CA. We also conducted qPCR and Western blot to determine the pathway involved. Key findings We identified that CA selectively suppressed proliferation of human RCC cell line A498 but not the human embryonic kidney cell HEK293. Mechanistic studies showed that CA significantly induced apoptosis, as indicated by activation of caspase protein and increased ratio of pro-apoptotic protein Bax to anti-apoptotic protein Bcl-2 (P < 0.05). Furthermore, we found that PI3K/ Akt/mTOR signalling pathway is involved in the inhibitory effect of CA on A498 cells. Activation of this pathway increased proliferation and decreased apoptosis of A498 cells, exhibiting antagonism function against CA. Conclusion Our research firstly reports the efficacy of CA against RCC cells and elucidates the underlying molecular mechanisms. These findings indicate that CA is a potential agent for treating RCC.

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Antibiofilm and Anti-��-Lactamase Activities of Burdock Root Extract and Chlorogenic Acid against Klebsiella pneumoniae

Cited by 50 publications

References 37 publications

The protective effect of chlorogenic acid on bovine mammary epithelial cells and neutrophil function

The protective effect of chlorogenic acid on bovine mammary epithelial cells and neutrophil function

Terpinen-4-ol as an Antibacterial and Antibiofilm Agent against Staphylococcus aureus

Chlorogenic acid inhibits proliferation and induces apoptosis in A498 human kidney cancer cells via inactivating PI3K/Akt/mTOR signalling pathway

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