Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus

Liu, Yawen; Wang, Li

doi:10.3389/fmicb.2022.1060506

Cited by 5 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that treatment with PCA showed the inhibitory effects on biofilms formation of E. coli (Bernal-Mercado et al, 2018). In addition, treatment with PAL had the inhibitory effects on biofilms formation of Ralstonia solanacearum, Yersinia enterocolitica and Vibrio parahaemolyticus (Li et al, 2016;Liu and Wang, 2022;Meng et al, 2022). C. albicans biofilm is a cellular community encased in selfreleased extracellular polysaccharides (Rodrigues et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Procatechuic acid and protocatechuic aldehyde increase survival of Caenorhabditis elegans after fungal infection and inhibit fungal virulence

Yuan,

Wang,

Zhang

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

Protocatechuic acid (PCA) and protocatechuic aldehyde (PAL) are important phenolic compounds in plants. We here investigated their possible beneficial effect against fungal infection and the underlying mechanism. The model animal of Caenorhabditis elegans was used as host, and Candida albicans was used as fungal pathogen. The nematodes were first infected with C. albicans, and the PCA and PAL treatment were then performed. Post-treatment with 10–100 μM PCA and PAL suppressed toxicity of C. albicans infection in reducing lifespan. Accompanied with this beneficial effect, treatment with 10–100 μM PCA and PAL inhibited C. albicans accumulation in intestinal lumen. In addition, treatment with 10–100 μM PCA and PAL suppressed the increase in expressions of antimicrobial genes caused by C. albicans infection. The beneficial effect of PCA and PAL against C. albicans infection depended on p38 MAPK and insulin signals. Moreover, although treatment with 10–100 μM PCA and PAL could not exhibit noticeable antifungal activity, PCA and PAL treatment obviously suppressed biofilm formation, inhibited hyphal growth, and reduced expressions of virulence genes (ALS3, CaVps34, Vma7, Vac1, and/or HWP1) related to biofilm formation and hyphal growth in C. albicans. Therefore, our data demonstrated the potential of PCA and PAL post-treatment against fungal infection and fungal virulence.

show abstract

Section: Discussionmentioning

confidence: 99%

Procatechuic acid and protocatechuic aldehyde increase survival of Caenorhabditis elegans after fungal infection and inhibit fungal virulence

Yuan,

Wang,

Zhang

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Food-borne biofilmforming pathogens include E. coli, Salmonella spp., Serratia spp., Listeria monocytogenes, Vibrio spp. and Campylobacter jejuni [41][42][43][44][45]. Important Gram-positive biofilm producers include healthcare-associated pathogens such as Streptococcus spp., Enterococcus spp., and Staphylococcus spp.…”

Section: Biofilm Development and Occurrencementioning

confidence: 99%

Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms

2023

View full text Add to dashboard Cite

Most bacteria attach to biotic or abiotic surfaces and are embedded in a complex matrix which is known as biofilm. Biofilm formation is especially worrisome in clinical settings as it hinders the treatment of infections with antibiotics due to the facilitated acquisition of antibiotic resistance genes (ARGs). Environmental settings are now considered as pivotal for driving biofilm formation, biofilm-mediated antibiotic resistance development and dissemination. Several studies have demonstrated that environmental biofilms can be hotspots for the dissemination of ARGs. These genes can be encoded on mobile genetic elements (MGEs) such as conjugative and mobilizable plasmids or integrative and conjugative elements (ICEs). ARGs can be rapidly transferred through horizontal gene transfer (HGT) which has been shown to occur more frequently in biofilms than in planktonic cultures. Biofilm models are promising tools to mimic natural biofilms to study the dissemination of ARGs via HGT. This review summarizes the state-of-the-art of biofilm studies and the techniques that visualize the three main HGT mechanisms in biofilms: transformation, transduction, and conjugation.

show abstract

“…Actinomycin D was added to the plates at final concentrations of 0, 1/8 MIC, 1/4 MIC, and 1/2 MIC. After a one-hour drying period, the center of each plate was inoculated with 5 μL of a V. parahaemolyticus bacterial solution (1 × 10 6 CFU/mL), and the cultures were incubated at 37°C for 12 h (Liu and Wang, 2022). The motility diameter was measured using a ruler (mm), and pictures were captured using the Gel DOCTM XR + system (Bio-Rad).…”

Section: Fermentation Extraction Isolation and Spectroscopic Analysismentioning

confidence: 99%

Anti-Vibrio parahaemolyticus compounds from Streptomyces parvus based on Pan-genome and subtractive proteomics

Liu

Tian

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionVibrio parahaemolyticus is a foodborne pathogen commonly found in seafood, and drug resistance poses significant challenges to its control. This study aimed to identify novel drug targets for antibacterial drug discovery.MethodsTo identify drug targets, we performed a pan-genome analysis on 58 strains of V. parahaemolyticus genomes to obtain core genes. Subsequently, subtractive proteomics and physiochemical checks were conducted on the core proteins to identify potential therapeutic targets. Molecular docking was then employed to screen for anti-V. parahaemolyticus compounds using a in-house compound library of Streptomyces parvus, chosen based on binding energy. The anti-V. parahaemolyticus efficacy of the identified compounds was further validated through a series of experimental tests.Results and DiscussionPangenome analysis of 58 V. parahaemolyticus genomes revealed that there were 1,392 core genes. After Subtractive proteomics and physiochemical checks, Flagellar motor switch protein FliN was selected as a therapeutic target against V. parahaemolyticus. FliN was modeled and docked with Streptomyces parvus source compounds, and Actinomycin D was identified as a potential anti-V. parahaemolyticus agent with a strong binding energy. Experimental verification confirmed its effectiveness in killing V. parahaemolyticus and significantly inhibiting biofilm formation and motility. This study is the first to use pan-genome and subtractive proteomics to identify new antimicrobial targets for V. parahaemolyticus and to identify the anti-V. parahaemolyticus effect of Actinomycin D. These findings suggest potential avenues for the development of new antibacterial drugs to control V. parahaemolyticus infections.

show abstract

Antibiofilm effect and mechanism of protocatechuic aldehyde against Vibrio parahaemolyticus

Cited by 5 publications

References 50 publications

Procatechuic acid and protocatechuic aldehyde increase survival of Caenorhabditis elegans after fungal infection and inhibit fungal virulence

Procatechuic acid and protocatechuic aldehyde increase survival of Caenorhabditis elegans after fungal infection and inhibit fungal virulence

Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms

Anti-Vibrio parahaemolyticus compounds from Streptomyces parvus based on Pan-genome and subtractive proteomics

Contact Info

Product

Resources

About