Antibiofilm peptides against biofilms on titanium and hydroxyapatite surfaces

Wang, Dan; Haapasalo, Markus; Gao, Yuan; Ma, Jia; Shen, Ya

doi:10.1016/j.bioactmat.2018.06.002

Cited by 42 publications

(30 citation statements)

References 43 publications

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“…Nanotechnology through the combination of AMPs and nanoparticles (NPs) could improve the antimicrobial action of the peptides but also overcome their disadvantages [49,50,51]. Nanotechnology offers a number of promising opportunities to develop antimicrobial nanosystems that break the biofilm barrier and penetrate over significantly larger distances into biofilm, killing its microorganisms more than conventional antibiotics or AMPs.…”

Section: Nanoparticles Coated With Ampsmentioning

confidence: 99%

Biofilms: Novel Strategies Based on Antimicrobial Peptides

et al. 2019

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The problem of drug resistance is very worrying and ever increasing. Resistance is due not only to the reckless use of antibiotics but also to the fact that pathogens are able to adapt to different conditions and develop self-defense mechanisms such as living in biofilms; altogether these issues make the search for alternative drugs a real challenge. Antimicrobial peptides appear as promising alternatives but they have disadvantages that do not make them easily applicable in the medical field; thus many researches look for solutions to overcome the disadvantages and ensure that the advantages can be exploited. This review describes the biofilm characteristics and identifies the key features that antimicrobial peptides should have. Recalcitrant bacterial infections caused by the most obstinate bacterial species should be treated with a strategy to combine conventional peptides functionalized with nano-tools. This approach could effectively disrupt high density infections caused by biofilms. Moreover, the importance of using in vivo non mammalian models for biofilm studies is described. In particular, here we analyze the use of amphibians as a model to substitute the rodent model.

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Section: Nanoparticles Coated With Ampsmentioning

confidence: 99%

Biofilms: Novel Strategies Based on Antimicrobial Peptides

et al. 2019

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“…Among them, a small synthetic peptide termed innate defense regulator (IDR-)1018, which was derived by substantial modification of the bovine neutrophil host defense peptide bactenecin, captured our attention (Mansour et al 2015 ). As the most potent IDR that has been described, IDR-1018 can prevent the accumulation and accelerate the degradation of ppGpp via direct interaction with this signaling molecule (Rivas-Santiago et al 2013 ; Wang et al 2018 ). Based on this mechanism, IDR-1018 acquired broad anti-biofilm activity.…”

Section: Introductionmentioning

confidence: 99%

Design of a novel antimicrobial peptide 1018M targeted ppGpp to inhibit MRSA biofilm formation

et al. 2021

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Methicillin-resistant staphylococcus aureus (MRSA) and its biofilm infection were considered as one of the main international health issues. There are still many challenges for treatment using traditional antibiotics. In this study, a mutant peptide of innate defense regulator (IDR-)1018 named 1018M was designed based on molecular docking and amino acid substitution technology. The antibacterial/biofilm activity and mechanisms against MRSA of 1018M were investigated for the first time. The minimum inhibitory concentration (MIC) of 1018M was reduced 1 time (MIC = 2 μg/mL) compared to IDR-1018. After treatment with 32 μg/mL 1018M for 24 h, the percentage of biofilm decreased by 78.9%, which was more effective than the parental peptide. The results of mechanisms exploration showed that 1018M was more potent than IDR-1018 at destructing bacterial cell wall, permeating cell membrane (20.4%–50.1% vs 1.45%–10.6%) and binding to stringent response signaling molecule ppGpp (increased 27.9%). Additionally, the peptides could also exert their activity by disrupting genomic DNA, regulating the expression of ppGpp metabolism and biofilm forming related genes (RSH, relP, relQ, rsbU, sigB, spA, codY, agrA and icaD). Moreover, the higher temperature, pH and pepsase stabilities provide 1018M better processing, storage and internal environmental tolerance. These data indicated that 1018M may be a potential candidate peptide for the treatment of MRSA and its biofilm infections.

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“…Finally, to determine if anti‐biofilm activity occurred with biofilms growing on other material surfaces that may be present in the oral cavity, the activity of DJK5 and IDR‐1018 peptides against biofilms grown on hydroxyapatite and titanium surfaces was compared. In both cases, DJK5 was more effective and killed more bacteria than IDR‐1018, however, there was no significant difference in the extent of killing by either peptide on hydroxyapatite versus titanium surface (Wang, Haapasalo, Gao, Ma, & Shen, ). In summary, these results suggest that IDR‐1018 and some of its derivative peptide mimetics, especially DJK5 and DJK6, may represent novel therapeutics for treating chronic biofilm infections at a variety of tissue sites and in the oral cavity.…”

Section: Antimicrobial Peptide‐based Mimeticsmentioning

confidence: 98%

Peptide and non‐peptide mimetics as potential therapeutics targeting oral bacteria and oral biofilms

Sztukowska

Roky

Demuth

2019

Molecular Oral Microbiology

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The development of the oral biofilm requires a complex series of interactions between host tissues and the colonizing bacteria as well as numerous interspecies interactions between the organisms themselves. Disruption of normal host–microbe homoeostasis in the oral cavity can lead to a dysbiotic microbial community that contributes to caries or periodontal disease. A variety of approaches have been pursued to develop novel potential therapeutics that are active against the oral biofilm and/or target specific oral bacteria. The structure and function of naturally occurring antimicrobial peptides from oral tissues and secretions as well as external sources such as frog skin secretions have been exploited to develop numerous peptide mimetics and small molecule peptidomimetics that show improved antimicrobial activity, increased stability and other desirable characteristics relative to the parent peptides. In addition, a rational and minimalist approach has been developed to design small artificial peptides with amphipathic α‐helical properties that exhibit potent antibacterial activity. Furthermore, with an increased understanding of the molecular mechanisms of beneficial and/or antagonistic interspecies interactions that contribute to the formation of the oral biofilm, new potential targets for therapeutic intervention have been identified and both peptide‐based and small molecule mimetics have been developed that target these key components. Many of these mimetics have shown promising results in in vitro and pre‐clinical testing and the initial clinical evaluation of several novel compounds has demonstrated their utility in humans.

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Antibiofilm peptides against biofilms on titanium and hydroxyapatite surfaces

Cited by 42 publications

References 43 publications

Biofilms: Novel Strategies Based on Antimicrobial Peptides

Biofilms: Novel Strategies Based on Antimicrobial Peptides

Design of a novel antimicrobial peptide 1018M targeted ppGpp to inhibit MRSA biofilm formation

Peptide and non‐peptide mimetics as potential therapeutics targeting oral bacteria and oral biofilms

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