Antibiogram of <i>Escherichia coli</i> and <i>Pseudomonas</i> Strains Isolated from Wastewater Generated by an Abattoir as It Journeys into a Receiving River

Abstract

Untreated wastewater from abattoir operations contains nutrients and other components that aid the growth of microorganisms especially bacteria. They also serve as a habitat for potentially pathogenic bacteria which might be a source of public health concern. The study was carried out to determine the antibiotics susceptibility profile of Gram-negative bacteria (Pseudomonas and Escherichia coli) to selected antibiotics. Wastewater samples were collected from ten different sampling points and cultured on Eosin Methylene Blue (EMB) and King's B medium. The bacterial strains obtained from the wastewater samples were subjected to antibiotics susceptibility tests, using the disc diffusion technique. A total of 60 Pseudomonas and 100 Escherichia coli were isolated out of which none of the Pseudomonas strains showed resistance to imipenem, colistin sulphate, meropenem and aztreonam, while 100% resistance was observed to ceftazidime and piperacillin. All the Escherichia coli strains were resistant to oxacillin and ceftazidime, while the percentage resistance to aztreonam, ertapenem, cefoxitin and tetracyline was 6%, 11%, 43% and 58% respectively. Eighty-five percent (85%) of the total Escherichia coli showed resistance to more than two antibiotics, while 14% showed resistance to ceftazidime and oxacillin, with only one isolate showing resistance to ceftazidime and cefoxitin. There is the need for an effective treatment of wastewater generated from abattoir operations to prevent the potential spread and transmission of antibiotic resistant bacteria to the human population who depends heavily on some of the water bodies, receiving input from abattoir wastes.

Isolation rate and amtimicrobial susceptibility profile of Enterobacteriaceae isolated from wastewater of Jimma Medical Center, Jimma Southwest Ethiopia

“…The current study revealed that the majority (88.6%) of bacterial isolates were multidrug resistant (MDR) which is in line with study conducted in China (85.5%) [13], in Ibadan, Nigeria (94.8%) [25], in Northwestern Ethiopia (81.5%) [17], in Mekelle, Northern Ethiopia (76.5%), in Addis Ababa Ethiopia (64%) [22], and in Arba Minch, Ethiopia (70.4%) [23]. The patterns of MDR antibiogram in the current study showed that: all (100%) isolates of Shigella spp., 93.5% of E. coli, 92.8% of Salmonella spp.,88.9% of Klebssiella spp.…”

Antibacterial effect of ethanolic extracts of Moroccan plant against Escherichia coli

“…Therefore, cefotaxime (5 µg) was the most effective antibacterial agent against Escherichia coli isolates. Recent investigations have reported the multiresistance of Escherichia coli to antibiotics [40]. Similar observations have been reported by Coleman [37].…”

“…Phenolic contents for these extracts ranged between 100.69±3. 40 Merr. & L. M. Perry, Cinnamomum zeylanicum Blume, Laurus nobilis L., Juniperus foetidissima Willd., Allium sativum L. and Myristica fragrans Houtt) against the gram-negative bacteria.…”

Antibiotic resistance profile of Pseudomonas aeruginosa isolated from aquaculture and abattoir environments in urban communities

“…Similar antibiotic resistance profile was also detected by Odjadjare et al [5] where high level resistance was observed against the penicillins (90%-100%), cephems (70%), sulphamethoxazole (90%) and rifampin (90%). Also, Falodun and Adekanmbi [22] revealed 100% resistance to ceftazidime and piperacillin.…”