Antibiotic resistance profile of Pseudomonas aeruginosa isolated from aquaculture and abattoir environments in urban communities

Igbinosa, Isoken H.; Beshiru, Abeni; Igbinosa, Etinosa O.

doi:10.12980/apjtd.7.2017d6-363

Cited by 29 publications

(25 citation statements)

References 13 publications

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“…In this study, 65.4% of the isolates recovered were P. aeruginosa, 27.3% were P. uorescens, while Burkholderia gladioli and Burkholderia cepacia constituted 5.5% and 1.8% respectively from abattoir wastewater and surface water (Mthatha dam) during the study period. In agreement with our studies, Igbinosa et al, Ejikeugwu et al and Tapela and Rahube, [6,31,32] have all reported the occurrence of P. aeruginosa and other species from hospital drains, environmental, and wastewater networks from various parts of the world. The occurrence of these microorganisms is a cause of concern given that they are opportunistic human pathogens and can infect people whose immunity are compromised [33].…”

Section: Isolation and Antimicrobial Susceptibility Testingsupporting

confidence: 93%

“…It has been designated as an emerging waterborne pathogen of economic, health and ecological relevance. It colonizes moist environment and preferentially lives in aquatic habitats such as rivers, wastewater and recreational waters [1,4,6].…”

mentioning

confidence: 99%

“…On the contrary, in developing countries the use of antibiotics in animal production is still on the rise. Recent research ndings revealed that occurrence of antimicrobial resistance in food-producing animals has nearly tripled within the last twenty years [6,17].…”

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confidence: 99%

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Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

Hosu¹,

Vasaikar²,

Okuthe³

et al. 2020

Preprint

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Background Pseudomonas aeruginosa (P. aeruginosa) are Gram-negative bacilli that are ubiquitous in nature particularly plentiful in soils and in aquatic milieu because they thrive well in humid or wet environments. It has extensive metabolic versatility, ability to thrive well and colonize diverse ecological habitats such as soil, rhizosphere, wastewaters that, enhances its potential threat to public health. Abattoirs, aquatic ecosystem including generated wastewaters are latent sources of pathogenic bacteria, serve as reservoirs and contribute to the spread of antibiotic resistant genes. Several studies have focused on clinical environment while scarce data exist from non-clinical environments, which sometimes are hotspots of antibiotic resistance. Thus, the present investigation aimed to identify antibiotic resistant gene of P. aeruginosa from non-clinical sources in Mthatha, Eastern Cape and evaluate its public health implications. Results Fifty-five (55) Pseudomonas species and other organisms recovered from effluent and surface water samples included P. aeruginosa (65.4%), P. fluorescens (27.3%), Burkholderia gladioli (5.5%) and Burkholderia cepacia (1.8%). The P. aeruginosa isolates showed high resistance to aztreonam (86.1%), ceftazidime (63.9%), piperacillin (58.3%) and cefepime (55.6%); with moderate resistance displayed to imipenem (50%), piperacillin/tazobactam (47.2%), meropenem (41.7%) and levofloxacin (30.6%). Twenty out of thirty-six (36) P. aeruginosa displayed multidrug resistance profiles and were classified as multidrug resistant (MDR) (55.6%). Most of the bacterial isolates exhibited a high Multiple Antibiotic Resistance (MAR) Index of > 0.2 ranging from 0.08–0.69 with a mean MAR Index of 0.38. PCR analysis of fifteen (15) P. aeruginosa isolates detected 14 (93.3%) harbored blaSHV, 6 (40%) harbored blaTEM with the least occurring ESBL as blaCTX−M at 3 (20%). Conclusions Results of the current study indicating resistance of environmental P. aeruginosa isolates to front-line clinically relevant antipseudomonal drugs is concerning and poses risk to the environment and receiving water bodies. Given the public health relevance, the results of this study highlight the importance and urgent need of monitoring multidrug-resistant pathogens in effluent environments. These non-clinical environments are potential reservoirs of resistance genes that would further serve as avenues for the dissemination of multidrug resistant bacteria in the community.

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Section: Isolation and Antimicrobial Susceptibility Testingsupporting

confidence: 93%

mentioning

confidence: 99%

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Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

Hosu¹,

Vasaikar²,

Okuthe³

et al. 2020

Preprint

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“…Pseudomonas aeruginosa (P. aeruginosa) is a Gram-negative rod shaped bacterium, which has a remarkable ability to adapt and thrive in a variety of environments, such as water, soil, occupational places, clinical settings, hospital, municipal wastewater and industrial effluents [3,4]. P. aeruginosa is widely distributed in nature, but has a higher prevalence in hospital environment.…”

Section: Introductionmentioning

confidence: 99%

“…The characteristic features of P. aeruginosa isolates that allow their persistence in hospital environment include its ability to acquire resistance to varieties of antibiotics, withstanding physical conditions like temperature, high concentration of salts and antiseptics [5,6]. P. aeruginosa has been reported to be resistant to antibiotics such as fluoroquinolone [4] and it is naturally resistant to many antibiotics due to the low permeability barrier afforded by its outer membrane, the action of multidrug efflux pumps and its tendency to colonize surfaces in a biofilm form making the cells impervious to therapeutic concentrations of antibiotics [7]. The simple and rapid detection of P. aeruginosa is essential in environmental monitoring and assessment and by extension, the protection of public health.…”

Section: Introductionmentioning

confidence: 99%

Antibiogram profile of Pseudomonas aeruginosa isolated from some selected hospital environmental drains

Imanah¹,

Beshiru²,

Igbinosa³

2017

APJTD

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The journal implements double-blind peer review practiced by specially invited international editorial board members. Objective: To isolate, identify and characterize Pseudomonas aeruginosa (P. aeruginosa) from hospital drains using culture-based and PCR methods. Methods: Wastewater samples were obtained from hospital drains between August and October, 2015, using standard culture-based methods for isolation of P. aeruginosa. The isolates were further confirmed by specie-specific primer sets. Antimicrobial susceptibility testing of the isolates was conducted using the Kirby-Bauer disc diffusion method. Results: The mean P. aeruginosa population densities were expressed ranging between (1.7 × 10 5 ± 0.1) and (6.1 × 10 5 ± 0.2) CFU/mL. The antimicrobial susceptibility profile of the P. aeruginosa isolates revealed that all the isolates 96/96 (100%) were resistant to penicillins (amoxicillin and cloxacillin) as well as penicillin/β-lactamase inhibitor (augmentin) while sensitivity was observed in carbapenems [imipenem 96/96 (100%)]. The multiple antimicrobial resistance index of the P. aeruginosa to the antimicrobials used ranged from 0.33 to 0.89 while the multidrug resistant profile revealed resistance to augmentin, amoxicillin, cloxacillin. Conclusions: The present study reveals that hospital drains are potential reservoirs of multiple antibiotic resistances of P. aeruginosa.

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The Current Status of Antibiotic-Resistant Bacteria and Resistance Genes in African Aquaculture

Limbu

2023

Antimicrobial Research and One Health in Africa

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Antibiotic resistance profile of Pseudomonas aeruginosa isolated from aquaculture and abattoir environments in urban communities

Cited by 29 publications

References 13 publications

Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

Antibiogram profile of Pseudomonas aeruginosa isolated from some selected hospital environmental drains

The Current Status of Antibiotic-Resistant Bacteria and Resistance Genes in African Aquaculture

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