Antibiogram profile of Pseudomonas aeruginosa isolated from some selected hospital environmental drains

Abstract

The journal implements double-blind peer review practiced by specially invited international editorial board members. Objective: To isolate, identify and characterize Pseudomonas aeruginosa (P. aeruginosa) from hospital drains using culture-based and PCR methods. Methods: Wastewater samples were obtained from hospital drains between August and October, 2015, using standard culture-based methods for isolation of P. aeruginosa. The isolates were further confirmed by specie-specific primer sets. Antimicrobial susceptibility testing of the isolates was conducted using the Kirby-Bauer disc diffusion method. Results: The mean P. aeruginosa population densities were expressed ranging between (1.7 × 10 5 ± 0.1) and (6.1 × 10 5 ± 0.2) CFU/mL. The antimicrobial susceptibility profile of the P. aeruginosa isolates revealed that all the isolates 96/96 (100%) were resistant to penicillins (amoxicillin and cloxacillin) as well as penicillin/β-lactamase inhibitor (augmentin) while sensitivity was observed in carbapenems [imipenem 96/96 (100%)]. The multiple antimicrobial resistance index of the P. aeruginosa to the antimicrobials used ranged from 0.33 to 0.89 while the multidrug resistant profile revealed resistance to augmentin, amoxicillin, cloxacillin. Conclusions: The present study reveals that hospital drains are potential reservoirs of multiple antibiotic resistances of P. aeruginosa.

Association of exopolysaccharide genes in biofilm developing antibiotic-resistant Pseudomonas aeruginosa from hospital wastewater

“…Pseudomonas spp. has a higher prevalence in hospital settings, hospital wastes, and their resistance is increasingly being recognized as a serious threat (Imanah et al 2017;Divyashree et al 2020). Several studies have reported the presence of antibiotic-resistant Pseudomonas spp.…”

Antibiotic resistant gene of Pseudomonas aeruginosa from non-clinical environment: public health implications in Mthatha, Eastern Cape Province, South Africa

“…The pathogenicity of P. aeruginosa favours its resistance to potent antimicrobials thereby resulting in therapeutic challenge and elongation of patients' stay in the hospital settings [39]. Table 1 shows the resistance pro les of the isolates and reveals 63.9% and 55.6% resistance to second and third generation cephalosporin respectively (ceftazidime and cefepime) and low resistance to aminoglycosides.…”

Antibiotic resistance of Pseudomonas aeruginosa isolated from hospital wastewater in the Czech Republic

“…P. aeruginosa is intrinsically resistant to many antibiotics and is capable of easily acquired antibiotic resistance determinants (Feng et al 2017;Imanah et al 2017). Furthermore, P. aeruginosa has a high potential to evolve multidrug resistance phenotypes (Golle et al 2017).…”

“…The presence of different resistance mechanisms has a significant clinical impact, since it limits the therapeutic options for P. aeruginosa infections, compromises the efficacy of antipseudomonal agents, and makes P. aeruginosa infection very difficult to treat (Feng et al 2017;Golle et al 2017;Azam & Khan 2019;Rocha et al 2019). P. aeruginosa strains have been reported to be resistant to a wide range of currently available antimicrobial agents, such as fluoroquinolones, but also carbapenems and third-generation cephalosporins, which are preferred options in the therapy of serious infections caused by MDR strains (Imanah et al 2017;Azam & Khan 2019).…”