Antibiotic and deoxycholate resistance in <i>Campylobacter jejuni</i> following freezing or heating

Humphrey, Tom J.; Cruickshank, J. G.

doi:10.1111/j.1365-2672.1985.tb01777.x

Cited by 49 publications

(23 citation statements)

References 28 publications

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“…It has been shown previously that freezing of campylobacter cultures results in a rapid decrease in viability (7,8). Furthermore, the results of a recent study carried out with yeast cells provided convincing evidence that reactive oxygen intermediates are generated during the freeze-thaw process and that these intermediates contribute to the lethal damage to the cell (11).…”

supporting

confidence: 48%

Roles of Fe Superoxide Dismutase and Catalase in Resistance of Campylobacter coli to Freeze-Thaw Stress

Stead

Park

2000

Appl Environ Microbiol

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supporting

confidence: 48%

Roles of Fe Superoxide Dismutase and Catalase in Resistance of Campylobacter coli to Freeze-Thaw Stress

Stead

Park

2000

Appl Environ Microbiol

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“…To establish whether this was due to the presence of organisms with increased cold resistance, the effects of refrigerated storage on viability and antibiotic sensitivity in these and other strains were measured using reported methods (Humphrey & Cruickshank, 1985).…”

Section: Examination Of Milk Samplesmentioning

confidence: 99%

“…In common with other bacteria, C. jejuni can be sub-lethally damaged by exposure to low temperature (Humphrey & Cruickshank, 1983; Humphrey, 1984;Ray & Johnson, 1984a, b;Humphrey & Cruickshank, 1985). This renders the organism both sensitive to certain selective agents commonly used in isolation and enrichment media, and less able to grow at routinely used high (42)(43) 'C) incubation temperatures (Humphrey, 1986a).…”

Section: Introductionmentioning

confidence: 99%

Campylobacter jejuniin dairy cows and raw milk

Humphrey

Beckett

1987

Epidemiol. Infect.

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SUMMARYTwelve herds of dairy cows were examined by rectal swabbing for the presence of Campylobacter jejuni. Ten herds were positive with the incidence of colonized animals ranging from 10 to 72 % of those tested. With the exception of the two negative herds where mains water only was consumed, all animals drank from rivers or streams when grazing. There was no relationship between total and coliform counts and the presence of C. jejuni in raw milk. However, milk from one farm that consistently gave positive results had significantly higher Escherichia coli counts than other samples.

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“…Sublethally injured cells were defined as those unable to tolerate 0.1% sodium deoxycholate (DOC) or 1% sodium chloride in the growth medium (15). The proportion of sublethally injured cells in the population was thus estimated by comparing plate counts on BMHA and MHA containing 0.1% (wt/vol) DOC (MHAD) or 1% (wt/vol) sodium chloride (MHAN).…”

Section: Methodsmentioning

confidence: 99%

Survival of Campylobacter jejuni during Stationary Phase: Evidence for the Absence of a Phenotypic Stationary-Phase Response

Kelly

Park

Bovill

et al. 2001

Appl Environ Microbiol

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When Campylobacter jejuni NCTC 11351 was grown microaerobically in rich medium at 39°C, entry into stationary phase was followed by a rapid decline in viable numbers to leave a residual population of 1% of the maximum number or less. Loss of viability was preceded by sublethal injury, which was seen as a loss of the ability to grow on media containing 0.1% sodium deoxycholate or 1% sodium chloride. Resistance of cells to mild heat stress (50°C) or aeration was greatest in exponential phase and declined during early stationary phase. These results show that C. jejuni does not mount the normal phenotypic stationary-phase response which results in enhanced stress resistance. This conclusion is consistent with the absence of rpoS homologues in the recently reported genome sequence of this species and their probable absence from strain NCTC 11351. During prolonged incubation of C. jejuni NCTC 11351 in stationary phase, an unusual pattern of decreasing and increasing heat resistance was observed that coincided with fluctuations in the viable count. During stationary phase of Campylobacter coli UA585, nonmotile variants and those with impaired ability to form coccoid cells were isolated at high frequency. Taken together, these observations suggest that stationary-phase cultures of campylobacters are dynamic populations and that this may be a strategy to promote survival in at least some strains. Investigation of two spontaneously arising variants (NM3 and SC4) of C. coli UA585 showed that a reduced ability to form coccoid cells did not affect survival under nongrowth conditions.

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Antibiotic and deoxycholate resistance in Campylobacter jejuni following freezing or heating

Cited by 49 publications

References 28 publications

Roles of Fe Superoxide Dismutase and Catalase in Resistance of Campylobacter coli to Freeze-Thaw Stress

Roles of Fe Superoxide Dismutase and Catalase in Resistance of Campylobacter coli to Freeze-Thaw Stress

Campylobacter jejuniin dairy cows and raw milk

Survival of Campylobacter jejuni during Stationary Phase: Evidence for the Absence of a Phenotypic Stationary-Phase Response

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