Antibiotic and Heavy Metal Resistance of Escherichia coli Strains Isolated from the Seve Dam, and Konak Pond, Kilis, Turkey

Toplar, Sevil; Özdenefe, Melis Sümengen

doi:10.22392/actaquatr.801564

Cited by 8 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of fecal coliforms in aquatic environments indicates the contamination of waters with the fecal material of man or other animals. Several previous studies have also demonstrated a higher concentration of total and fecal coliforms in different water bodies in Turkey (Buyukkaya Kayis et al 2017;Avsar 2018;Hulyar and Altug 2020;Mercimek Takcı et al 2021). Our another a study performed for different locations at same time in Kilis observed fecal streptococ contamination as 11 EMS/100 mL (Mercimek Takci and Toplar 2023).…”

Section: Resultsmentioning

confidence: 80%

Antibiotic and Heavy Metal Resistance Patterns of Indicator Bacteria in Surface Water Bodies of Kilis

Mercimek Takcı,

Toplar

2023

International Journal of Environment and Geoinformatics

Self Cite

View full text Add to dashboard Cite

The surface waters contaminated with coliform bacteria having antibiotic and heavy metal resistance have become an increasing public health risk. For this reason, it is aimed to detect the bacterial quality, the frequency of antibiotics, heavy metal resistance, and bioindicator bacteria in surface water sources taken from Kilis. The resistance profile of sixteen bacteria species belonging to class Gammaproteobacteria to standard antibiotics and heavy metal salts was investigated using Kirby-Bauer disc diffusion techniques. The various physicochemical parameters such as total dissolved solids (TDS), electrical conductivity (EC), pH, temperature, dissolved oxygen amount, and biochemical oxygen demand (BOD) of samples were also examined. The total coliform load was recorded as˃1100 (MPN)/100 mL and calculated comparatively lower values (53(MPN)/100 mL) of fecal contamination for both stations. A high level of resistance to clindamycin in a total of 16 strains was observed. Ampicillin (56.25%), cefotaxime (37.5%), and ceftazidime (31.25%) followed them. The trends in heavy metal resistance of isolates increased in the order of Cd2+< Pb2+

show abstract

Section: Resultsmentioning

confidence: 80%

Antibiotic and Heavy Metal Resistance Patterns of Indicator Bacteria in Surface Water Bodies of Kilis

Mercimek Takcı,

Toplar

2023

International Journal of Environment and Geoinformatics

Self Cite

View full text Add to dashboard Cite

show abstract

“…This might show that the main source of contamination in the Orontes River is domestic sewage and solid residues from surrounding densely populated areas. There are many reports on the existence of fecal coliforms in various aquatic environments in Turkey [19][20][21]. The highest total coliform and fecal coliform levels were reported to be 13x10 highlighted that indicated the uncontrolled discharges with 940x10 3 CFU/100 mL total coliform value at Alibeykoy Creek [23].…”

Section: Resultsmentioning

confidence: 99%

Antibiotic susceptibility profiles of Escherichia coli strains and fecal contamination in Orontes River, Turkey

KARACA,

HÜNER,

MERCİMEK TAKCI

2023

International Journal of Life Sciences and Biotechnology

Self Cite

View full text Add to dashboard Cite

This study was to evaluate the level of contamination of surface waters by coliform bacteria and Escherichia coli, as well as to detect the fecal pollution and the antibiotic resistance patterns of E. coli strains from recovered Asi (Orentes) River in the southern region of Turkey. Total and fecal coliform results were revealed as ˃1100 MPN/100 mL and 1100 MPN/100 mL for two stations, respectively. A total of 10 E. coli isolates were screened for antibiotic susceptibility by Kirby Bauer method. Resistance to cefotaxime, tobramycin, streptomycin, trimethoprim, cefepime, cefazolin, and ampicillin was determined in 20%, 20%, 40%, 20%, 20%, 20%, and 40%, respectively. These findings point out a widespread distribution of E. coli strains produced antibiotic resistance genes in the surface waters in the southern region of Turkey, suggesting an aquatic reservoir for resistance genes. Multiple antibiotic resistance (MAR) rates of %30 of E. coli isolates were greater than 0.200. This high index indicates exceeding the permissible water quality limits for human use and consumption and is the primary source of contamination in the discharges from domestic, agricultural, and urban.

show abstract

“…Another important aspect of this manuscript is the determination of antibiotic susceptible pro les in Gr negative bacteria isolated from the Alleben water sample. Although the prevalence of antibiotic-resistant bacteria (ARB) in many freshwater sources of Turkey has been previously demonstrated [32][33], this is the rst study that indicated the high frequency of resistance in the surface waters of Alleben, Gaziantep.…”

Section: Discussionmentioning

confidence: 99%

A Comparison Study of Quantitative PCR and Conventional Culture-Based Methods for Microbial Water Quality

KARACA

2022

Preprint

View full text Add to dashboard Cite

The goal of this study is to assess the microbial quality of water sources by using conventional culture-dependent microbiological methods and culture-independent molecular-based qPCR application for the monitoring of total bacteria, total coliform, and E. coli as an indicator of fecal contamination. In this experiment, a surface water sample was collected from Gaziantep, Turkey and the detection of microbial load and contamination was performed through gen-specific qPCR, plate count, and MPN quantification techniques. Total bacteria, total coliform, and E. coli levels using qPCR assay were significantly higher than that enumerated by culture-dependent microbiological methods in the water sample. Differences in the water pollution influenced the suitability of molecular method was clarified to the occurring viable but not cultivable (VBNC) bacteria and the measuring of low levels of DNA target by qPCR. The microbial quality of the Gaziantep Alleben water sample predicted by qPCR showed a positive correlation with traditional methods. These results stated that our real-time PCR approach can be suitable to estimate the relative abundances of uncultured and cultured indicator microorganisms affected by the water characteristics. The high level (100%) of resistance observed to clindamycin, erythromycin, penicillin G, tetracycline, and trimethoprim followed cefotaxime (62.5%), ceftazidime (50.0%), streptomycin (50.0%), ciprofloxacin (37.5%), and cefoperazone (25.0%) in a total of 8 strains. The high MAR index (0.33-0.67) of Gr negative strains indicated higher anthropogenic activity such as discharge points of industrial and domestic wastewaters which can contribute to microbiological contamination in surface waters.

show abstract

Antibiotic and Heavy Metal Resistance of Escherichia coli Strains Isolated from the Seve Dam, and Konak Pond, Kilis, Turkey

Cited by 8 publications

References 31 publications

Antibiotic and Heavy Metal Resistance Patterns of Indicator Bacteria in Surface Water Bodies of Kilis

Antibiotic and Heavy Metal Resistance Patterns of Indicator Bacteria in Surface Water Bodies of Kilis

Antibiotic susceptibility profiles of Escherichia coli strains and fecal contamination in Orontes River, Turkey

A Comparison Study of Quantitative PCR and Conventional Culture-Based Methods for Microbial Water Quality

Contact Info

Product

Resources

About