Antibiotic and heavy-metal resistance of Vibrio parahaemolyticus isolated from oysters in Korea

Kang, Chang-Ho; Shin, Yujin; Yu, Hong-Sik; Kim, SuKyung; So, Jae-Seong

doi:10.1016/j.marpolbul.2018.07.007

Cited by 29 publications

(21 citation statements)

References 31 publications

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“…The results showed that ICEs-positive isolates have been given more antibiotic resistance, and the MDR strains also showed more heavy metal resistance. Consistent with previous reports (Song et al, 2013; Hu and Chen, 2016; Kang et al, 2018), we found that AMP resistance dominated among the isolates and was present in all samples tested. Although TET, sulfonamides, and quinolones are used widely in aquaculture (Holmström et al, 2003), we did not find evidence of resistance to TET in the V. parahaemolyticus isolates.…”

Section: Resultssupporting

confidence: 93%

Integrative and Conjugative Elements-Positive Vibrio parahaemolyticus Isolated From Aquaculture Shrimp in Jiangsu, China

Wang

Zhang

et al. 2019

Front. Microbiol.

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The development of multidrug- and toxin-resistant bacteria as a result of increasing industrialization and sustained and intense antimicrobial use in aquaculture results in human health problems through increased incidence of food-borne illnesses. Integrative and conjugative elements (ICEs) are self-transmissible mobile genetic elements that allow bacteria to acquire complex new traits through horizontal gene transfer and encode a wide variety of genetic information, including resistance to antibiotics and heavy metals; however, there is a lack of studies of ICEs of environmental origin in Asia. Here, we determined the prevalence, genotypes, heavy metal resistance and antimicrobial susceptibility of 997 presumptive strains of Vibrio parahaemolyticus ( tlh + , tdh – ), a Gram-negative bacterium that causes gastrointestinal illness in humans, isolated from four species of aquaculture shrimp in Jiangsu, China. We found that 59 of the 997 isolates (5.9%) were ICE-positive, and of these, 9 isolates tested positive for all resistance genes. BLAST analysis showed that similarity for the eight strains to V. parahaemolyticus was 99%. Tracing the V. parahaemolyticus genotypes, showed no significant relevance of genotype among the antimicrobial resistance strains bearing the ICEs or not. Thus, in aquaculture, ICEs are not the major transmission mediators of resistance to antibiotics or heavy metals. We suggest future research to elucidate mechanisms that drive transmission of resistance determinants in V. parahaemolyticus .

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Section: Resultssupporting

confidence: 93%

Integrative and Conjugative Elements-Positive Vibrio parahaemolyticus Isolated From Aquaculture Shrimp in Jiangsu, China

Wang

Zhang

et al. 2019

Front. Microbiol.

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“…It has been reported that clinical isolates have higher rates of virulence genes than isolates from aquatic products, which may be due to environmental factors such as interactions with other hosts and the evolution of pathogens (Letchumanan et al, 2015;Xie et al, 2017). Additionally, tdh-positive isolates are more virulent than trh-positive isolates (Kang et al, 2018). In our study, none of the isolates from markets A and B were positive for tdh, which may represent a reduced risk for serious infections for consumers.…”

Section: Discussionmentioning

confidence: 48%

“…Thus, it is important to monitor variations in the antibiotic resistance profiles of V. parahaemolyticus isolates, as they may reveal changes in the sensitivity of the bacteria to antibiotics, particularly first-line treatments of seafood or human infections (Lopatek et al, 2018). In addition, industrial pollution caused by increased industrialization has become one of the most challenging issues facing developing countries (Kang et al, 2018). Among the many industrial pollutants, heavy metals are frequently detected in marine animals and in various environments, such as agricultural soil and rivers (Ansari et al, 2008;Malik and Aleem, 2011).…”

Section: Introductionmentioning

confidence: 99%

Co-occurrence of Antibiotic and Heavy Metal Resistance and Sequence Type Diversity of Vibrio parahaemolyticus Isolated From Penaeus vannamei at Freshwater Farms, Seawater Farms, and Markets in Zhejiang Province, China

Jiang

Yang

et al. 2020

Front. Microbiol.

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Vibrio parahaemolyticus is the leading cause of seafood-borne bacterial poisoning in China and is a threat to human health worldwide. The aim of this study was to assess the antibiotic resistance profiles and distribution of heavy metal resistance of V. parahaemolyticus isolates from Penaeus vannamei from freshwater farms, seawater farms, and their corresponding markets in Zhejiang, China and to assess the relationship between multidrug resistance (MDR) and multi-heavy metal resistance (MHMR). Of the 360 P. vannamei samples that we tested, 90 (25.00%) were V. parahaemolyticus positive, but the occurrence of pathogenic isolates carrying the toxin genes tdh (4.44%) and trh (3.33%) was low. None of the tested isolates harbored both the tdh and trh genes. However, antibiotic resistance profiles varied among different sampling locations, levels of resistance to the antibiotics ampicillin (76.67%) and streptomycin (74.44%) were high overall, and MDR isolates were common (40.00% of all isolates). Heavy metal resistance patterns were similar among the different sampling locations. Overall, the majority of V. parahaemolyticus isolates displayed tolerance to Cd 2+ (60.00%), and fewer were resistant to Cu 2+ (40.00%), Zn 2+ (38.89%), Ni 2+ (24.44%), Cr 3+ (14.44%), and Co 2+ (8.89%). In addition, 34.44% (31/90) of isolates tested in this study were found to be MHMR. Using Pearson's correlation analysis, MDR and MHMR were found to be positively correlated (P = 0.004; R = 0.759). The 18 V. parahaemolyticus isolates that were both MDR and MHMR represented 18 sequence types, of which 12 were novel to the PubMLST database, and displayed a high level of genetic diversity, suggesting that dissemination may be affected by mobile genetic elements via horizontal gene transfer. However, a low percentage of class 1 integrons without gene cassettes and no class 2 or 3 integrons were detected in the 18 MDR and MHMR isolates or in the

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“…Additionally, the quantified MAR value (0.2 to 0.6) demonstrates the effect of environmental contamination by antibiotics used to eradicate the pathogen. Kang et al (2018) suggest that a value greater than 0.2 found in food samples of marine origin is considered as high risk for human health. In our study, the average MAR value suggests a high exposure of the evaluated strain to antibiotics.…”

Section: Categorymentioning

confidence: 99%

Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination

Gámez-Bayardo

Castañeda-Ruelas

Espinosa-Plascencia

et al. 2021

Lat. Am. J. Aquat. Res.

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Vibrio parahaemolyticus is recognized as a human pathogen as well as the causative agent of vibriosis in shrimp. This study determined the pathogenic, antimicrobial, and biotic potential of V. parahaemolyticus isolated from white shrimp (Penaeus vannamei) and seawater on a northwestern Mexico farm. A total of 140 samples were randomly collected, including juvenile organisms (n = 120) and seawater (n = 20). The pH, salinity, and biota of the ponds were used to correlate with bacterium presence. The strains were characterized by virulence genes presence, biofilm formation capacity, antimicrobial sensitivity, and the kinetics growth using PCR, microplates method, minimum inhibitory concentration (MIC), and spectrophotometry, respectively. V. parahaemolyticus was detected in 7.1% of the samples with a mean concentration of 3.72 ± 1.24 log CFU mL-1; 6.7% (8/120) in shrimp and 10.0% (2/20) in seawater. Cultivation conditions were not predictive of the specie (P > 0.05). V. parahaemolyticus showed an adaptation time of 1.0 h, and a growth rate of 0.375 h-1 in seawater at 30ºC. The strains were classified into two pathotypes: tlh+/tdh-/trh-/AP2- (75%) and tlh+/tdh-/trh-/AP2+ (25%) and three resistant profiles to clinical drugs (ampicillin, amikacin, gentamicin, and netilmicin). The MIC values against oxytetracycline (OTC), florfenicol (FFC) and enrofloxacin (ENRO) were >0.50, >0.25 and >0.06 μg mL-1, respectively. Biofilm formation was a property identified in 40% of the strains. The presence of infectious V. parahaemolyticus with high adaptative potential justifies integrating integrated aquaculture practices and management to control pathogen growth and shrimp health.

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Antibiotic and heavy-metal resistance of Vibrio parahaemolyticus isolated from oysters in Korea

Cited by 29 publications

References 31 publications

Integrative and Conjugative Elements-Positive Vibrio parahaemolyticus Isolated From Aquaculture Shrimp in Jiangsu, China

Integrative and Conjugative Elements-Positive Vibrio parahaemolyticus Isolated From Aquaculture Shrimp in Jiangsu, China

Co-occurrence of Antibiotic and Heavy Metal Resistance and Sequence Type Diversity of Vibrio parahaemolyticus Isolated From Penaeus vannamei at Freshwater Farms, Seawater Farms, and Markets in Zhejiang Province, China

Characterization of Vibrio parahaemolyticus strains and evaluation of shrimp cultivation conditions in a farm at the northwestern of Mexico, as risk predictors for its adaptation and dissemination

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