Antibiotic-loaded reactive oxygen species-responsive nanomedicine for effective management of chronic bacterial prostatitis

Zheng, Jun; Hu, Ruimin; Yang, Yang; Wang, Yu; Wang, Qianmei; Xu, Senlin; Pu, Yulin; Li, Yong; Zhou, Jiangling; Yang, Jing; Bao, Ying; Zhang, Dinglin; Shen, Wenhao; Zhou, Zhansong

doi:10.1016/j.actbio.2022.02.044

Cited by 19 publications

(19 citation statements)

References 31 publications

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“…Because FRs have comparable affinity for FA, FA-modified drugs or biomolecules can actively target cells or tissues that overexpress FRs ( Muhamad et al, 2018 ). In our previous study ( Zheng et al, 2022 ), it was confirmed that FRs were highly expressed in the prostate tissues of CBP mice, providing an experimental basis for the active targeting of FA-modified NPs. Studies have shown that neutrophils can be recruited to lesion sites by inflammatory factors due to their sensitivity to the inflammatory environment ( Dorschner et al, 2020 ; Hou et al, 2022 ) and have a natural homing effect on prostatitis ( Seo et al, 2014 ; Ahn et al, 2018 ; Safari et al, 2020 ).…”

Section: Introductionmentioning

confidence: 53%

“…Escherichia coli (E. coli) isolates were obtained from patients with urinary infection at the First Affiliated Hospital of Army Medical University (Third Military Medical University), and E2519 was selected to establish a murine model of chronic prostatitis ( Zheng et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…10 μl of E. coli E2519 (1×10 8 colony forming units/mL) was injected into mice to establish a mouse model of CBP as described previously ( Zheng et al, 2022 ). Mice were maintained with urinary retention under anesthesia for at least 0.5 h to facilitate bacterial culture.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

“…As mentioned above, the physicochemical properties of NPs, such as particle size and surface modification ligands, determine their ability to passively target and actively target in vivo . In a previous study ( Zheng et al, 2022 ), our group first proved the feasibility of nanodrugs in the treatment of CBP, and verified the biosafety and effectiveness of nanodrugs at the cellular level and animal level, but the targeting capacities of NPs to inflammatory prostate tissues were not studied in depth. Since NPs penetrating the prostate epithelial barrier and achieving targeted delivery to prostate tissues are crucial to improve the efficacy of chronic prostatitis, it is of great significance to study the targeting capabilities of NPs with different physicochemical properties to prostate tissues, which may provide reference for targeted therapy of prostate-related diseases by nanotechnology in the future.…”

Section: Introductionmentioning

confidence: 99%

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In vivo targeting capacities of different nanoparticles to prostate tissues based on a mouse model of chronic bacterial prostatitis

Yang

Song

et al. 2022

Front. Bioeng. Biotechnol.

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Chronic bacterial prostatitis usually occurs in men and seriously affects the quality of life of patients. The efficacy of chronic bacterial prostatitis treatment is limited by the difficulty for free drugs (e.g., antibiotics) to penetrate the prostate epithelium and target inflammatory tissues. The advent of nanotechnology offers the possibility to address this issue, such as the development of targeted nanoparticle delivery strategies that may overcome these important limitations. The physicochemical properties of nanoparticles, such as particle size, shape and surface modification ligands, determine their targeting effectiveness. In this study, nanoparticles with different physicochemical properties were prepared to explore and confirm their targeting capacities to inflammatory prostate tissues of chronic bacterial prostatitis, focusing on the effects of size and different modification ligands on the targeting performance. In vivo and ex vivo imaging results verified that folic acid-modified nanoparticles with a particle size of 180–190 nm via tail intravenous injection had the optimal targeting efficiency to prostate tissues. Our results provide an experimental basis and reference value for targeted therapy of prostate-related diseases with nanotechnology in the future.

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Section: Introductionmentioning

confidence: 53%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vivo targeting capacities of different nanoparticles to prostate tissues based on a mouse model of chronic bacterial prostatitis

Yang

Song

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

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Ursolic acid alleviates chronic prostatitis via regulating NLRP3 inflammasome‐mediated Caspase‐1/GSDMD pyroptosis pathway

Liu,

Guo,

Gao

et al. 2023

Phytotherapy Research

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Ursolic acid (UA) is a naturally occurring pentacyclic triterpenoid widely found in fruits and vegetables. It has been reported that UA has anti‐inflammatory effects. However, its efficacy and mechanism of action in the treatment of chronic prostatitis (CP) remain unclear. This study aimed to investigate the efficacy of UA treatment in CP and further explore the underlying mechanism. CP rat and pyroptosis cell models were established in vivo and in vitro, respectively. The efficacy of UA in inhibiting CP was evaluated via haematoxylin‐eosin (HE) staining and measurement of inflammatory cytokines. RNA sequencing and molecular docking were used to predict the therapeutic targets of UA in CP. The expression of pyroptosis‐related proteins was examined using various techniques, including immunohistochemistry, immunofluorescence, and flow cytometry. UA significantly ameliorated pathological damage and reduced the levels of proinflammatory cytokines in the CP model rats. RNA sequencing analysis and molecular docking suggested that NLRP3, Caspase‐1, and GSDMD may be key targets. We also found that UA decreased ROS levels, alleviated oxidative stress, and inhibited p‐NF‐κB protein expression both in vivo and in vitro. UA improved pyroptosis morphology as indicated by electron microscope and inhibited the expression of the pyroptosis‐related proteins NLRP3, Caspase‐1, ASC, and GSDMD, reversed the levels of IL‐1β, IL‐18, and lactate dehydrogenase in vivo and in vitro. UA can mitigate CP by regulating the NLRP3 inflammasome‐mediated Caspase‐1/GSDMD pathway. Therefore, UA may be a potential for the treatment of CP.

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Glucocorticoid-loaded pH/ROS dual-responsive nanoparticles alleviate joint destruction by downregulating the NF-κB signaling pathway

Zhou

Wang

et al. 2023

Acta Biomaterialia

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Antibiotic-loaded reactive oxygen species-responsive nanomedicine for effective management of chronic bacterial prostatitis

Cited by 19 publications

References 31 publications

In vivo targeting capacities of different nanoparticles to prostate tissues based on a mouse model of chronic bacterial prostatitis

In vivo targeting capacities of different nanoparticles to prostate tissues based on a mouse model of chronic bacterial prostatitis

Ursolic acid alleviates chronic prostatitis via regulating NLRP3 inflammasome‐mediated Caspase‐1/GSDMD pyroptosis pathway

Glucocorticoid-loaded pH/ROS dual-responsive nanoparticles alleviate joint destruction by downregulating the NF-κB signaling pathway

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