Antibiotic resistance and antibiotic-resistance genes of <i>Pseudomonas</i> spp. and <i>Escherichia coli</i> isolated from untreated hospital wastewater

Asghari, Farzaneh Baghal; Dehghani, Mohammad Hadi; Dehghanzadeh, Reza; Farajzadeh, Davoud; Yaghmaeian, Kamyar; Mahvi, Amir Hossein; Rajabi, Akbar

doi:10.2166/wst.2021.207

Cited by 15 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, hospital wastewaters may represent major sources of pathogenic organisms in public wastewater, playing an important role regarding multidrug-resistant pathogens and also in the propagation of antibiotic resistance in the environment [7]. In fact, many studies have shown that hospital wastewaters contribute to the mobilization and dissemination of important nosocomial pathogens as well as antimicrobial resistance genes and genetic determinants which may reach surface waters, influencing the aquatic ecosystems and interfering with the food chain [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater

et al. 2022

View full text Add to dashboard Cite

Hospital wastewaters often carry multidrug-resistant bacteria and priority pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA). Pathogens and antibiotic resistance genes present in wastewaters may reach the natural environment facilitating their spread. Thus, we aimed to isolate MRSA from wastewater of 3 hospitals located in the north of Portugal and to characterize the isolates regarding the antimicrobial resistance and genetic lineages. A total of 96 wastewater samples were collected over six months. The water was filtered, and the filtration membrane was immersed in BHI broth supplemented with 6.5% of NaCl and incubated. The inoculum was streaked in ORSAB agar plates for MRSA isolation. The isolates susceptibility testing was performed against 14 antimicrobial agents. The presence of resistance and virulence genes was accessed by PCR. Molecular typing was performed in all isolates. From the 96 samples, 28 (29.2%) were MRSA-positive. Most isolates had a multidrug-resistant profile and carried the mecA, blaZ, aac(6′)-Ie-aph(2″)-Ia, aph(3′)-IIIa, ermA, ermB, ermC, tetL, tetM, dfrA dfrG and catpC221 genes. Most of the isolates were ascribed to the immune evasion cluster (IEC) type B. The isolates belonged to ST22-IV, ST8-IV and ST105-II and spa-types t747, t1302, t19963, t6966, t020, t008 and tOur study shows that MRSA can be found over time in hospital wastewater. The wastewater treatment processes can reduce the MRSA load. The great majority of the isolates belonged to ST22 and spa-type t747 which suggests the fitness of these genetic lineages in hospital effluents.

show abstract

Section: Introductionmentioning

confidence: 99%

High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Nutrient agar (NA) medium was used to cultivate THB and the bacterial count was determined using the plate count method. Primary isolation and identification of Pseudomonas spp ., Acinetobacter spp ., and E. coli were conducted by culturing on Cetrimide agar (CA) and Eosin Methylene Blue agar (EMB), respectively (Baghal Asghari et al, 2021; Banerjee et al, 2017). Different antibiotics in concentrations of 16, 32, 64, and 128 μg/ml were added in culture mediums after autoclaving, and subsequently the medium was distributed on plates.…”

Section: Methodsmentioning

confidence: 99%

Characterization of antibiotic resistance genes and bacteria in a municipal water resource recovery facility

Rajabi

Farajzadeh

Dehghanzadeh

et al. 2022

Water Environment Research

Self Cite

View full text Add to dashboard Cite

Municipal water resource recovery facilities (WRRFs) are important sources of antibiotic‐resistant bacteria and genes (ARB and ARGs). In this study, antibiotic‐resistant total heterotrophic bacteria (THBR) counts (CFU/ml) cultivated from influent, effluent of activated sludge process, and outflow of disinfection unit of an urban WRRF were investigated for the presence of 16, 32, 64, and 128 μg/ml of nine antibiotics. The isolates of Pseudomonas spp., Acinetobacter spp., and Escherichia coli obtained from effluent of activated sludge process were subjected for molecular identification by detecting the 16S rRNA gene sequences. Additionally, using the polymerase chain reaction method (PCR), the isolates were investigated for the presence of blaSHV, blaTEM, blaCTX‐M, blaVIM, sul1, and qnrS genes. According to the results, the abundance of THBR counts was not significantly reduced by the biological treatment except for cefixime and sulfamethoxazole; it also increased for some antibiotics after disinfection unit. The average removal efficiency of THBR resistant to ciprofloxacin, sulfamethoxazole, and ceftazidime were 7.9 ± 1.7%, 41.8 ± 2.1%, and 14.4 ± 6.2%, respectively. Also, all the tested isolates were resistant to at least four antibiotics. For all antibiotics, the resistance ratio (THBR/THB) significantly increased in the effluent and after chlorination unit. Among 12 resistant isolates, blaTEM and sul1 genes were the most frequently detected ones involved in 92% and 83% of the isolates, respectively. Both blaTEM and sul1 genes were found in 100% of E. coli, and 83% and 67% of Pseudomonas spp. isolates, respectively. Further efforts are necessary to limit the transmission of ARB and ARGs from WRRFs into the environment and prevent human health threats. Practitioner Points The ratio of resistance significantly increased after biological treatment. Up to 40% of heterotrophic bacteria in the effluent was antibiotic resistant. blaTEM and sul1 genes were more prevalent (92%) in all isolates of bacteria. Both blaTEM and sul1 genes were found in 100% of E. coli isolates. Pseudomonas spp. holds blaTEM and sul1 genes in 83% and 67% of isolates, respectively.

show abstract

“…Multiple dilutions were performed up until a 10 -6 dilution was obtained. Finally, 1 mL of the 10 -6 dilution was inoculated onto Eosin Methylene Blue (EMB, Oxoid) agar medium, followed by incubation for 24 hours at 37°C (Asghari et al 2021).…”

Section: Isolation Characterization and Quantification Of E Colimentioning

confidence: 99%

Abundance and phenotypic-genotypic analysis of antibiotic-resistant Escherichia coli isolated from wastewater of the Zainoel Abidin Hospital, Banda Aceh, Indonesia

SUHARTONO

Hayati

2023

Biodiversitas

View full text Add to dashboard Cite

Abstract. Anelia N, Suhartono S, Hayati Z. 2023. Abundance and phenotypic-genotypic analysis of antibiotic-resistant Escherichia coli isolated from wastewater of the Zainoel Abidin Hospital, Banda Aceh, Indonesia. Biodiversitas 24: 2294-2301. Excessive and inappropriate use of antibiotics can initiate the emergence of antibiotic resistance in bacteria. Wastewater Treatment Plant (WWTP) is believed to be a reservoir for antibiotic-resistant bacteria, including Escherichia coli. This study aimed to perform the enumeration, isolation, characterization, and evaluation of resistance properties and antibiotic resistance-coding genes encoded by a plasmid of E. coli isolates from WWTP of the dr. Zainoel Abidin General Hospital (RSUDZA). The study was conducted through several stages, including the isolation and quantification of E. coli, antibiotic resistance testing, confirmation testing of ESBL-E. coli isolates, isolation of plasmid DNA, and amplification of TEM, SHV, and CTX-M genes. The results showed that the wastewater in the sum pit tank, aeration tank, and final tank of the WWTP contained E. coli at 7.5x104 CFU/mL, 3x102 CFU/mL, and 1x102 CFU/mL, respectively. A total of 22 isolates were obtained, with ten isolates from the sum pit tank, seven from the aeration tank, and five from the final tank. Escherichia coli isolates had resistance to eight types of antibiotics in the aeration tank and seven types in the final tank, compared to the sum pit tank of the WWTP. Therefore, 59% of the isolates were classified as Multi-drug Resistant (MDR) and 32% as Extended Spectrum ?-Lactamase E. coli (ESBL-EC). TEM, SHV, and CTX-M genes were detected in all locations at 63.6%, 22.7%, and 95.5%, respectively. In addition, CTX-M was the most common gene found in wastewater isolates at RSUDZA WWTP. The hospital wastewater treatment process must be improved to reduce the presence of antibiotic-resistant bacteria and the spread of antibiotic resistance genes.

show abstract

Antibiotic resistance and antibiotic-resistance genes of Pseudomonas spp. and Escherichia coli isolated from untreated hospital wastewater

Cited by 15 publications

References 29 publications

High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater

High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater

Characterization of antibiotic resistance genes and bacteria in a municipal water resource recovery facility

Abundance and phenotypic-genotypic analysis of antibiotic-resistant Escherichia coli isolated from wastewater of the Zainoel Abidin Hospital, Banda Aceh, Indonesia

Contact Info

Product

Resources

About