Antibiotic resistance and molecular characterization of poultry isolates of Salmonella by RAPD-PCR

Thakur, Youg Raj; Bajaj, Bijender Kumar

doi:10.1007/s11274-006-9159-8

Cited by 18 publications

(15 citation statements)

References 13 publications

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“…The successful use of antibiotics in veterinary medicine not withstanding its use has become particularly worrisome, especially for the fact of the potential to extend such drug into the human food chain or the possibility of reduced efficacy of such drugs which has been observed in some reports to be administered by non-qualified personnel (Boonmar et al, 1998, Thakur andBajaj, 2006). A recent survey by Alao and Ojo (2007) in Ekiti State, Nigeria, indicate a gradual increase in the use of various antibiotics like the quinolones, gentamycin, neomycin and chloramphenicol which were not normally previously used before now in livestock medicine.…”

Section: Discussionmentioning

confidence: 99%

Antibiograms of salmonella isolates from poultry farms in Ovia North East local government area, Edo State, Nigeria

Enabulele¹,

Amune²,

Aborisade³

2010

ABJNA

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Water, fecal and swab samples of poultry walls and cages collected from various poultry farms located in five towns and villages in Ovia North east local government area of Edo State, Nigeria, were screened for the presence of Salmonella spp and antibiotic resistance pattern of the isolates. The prevalence rates recorded shows that Salmonella was isolated from all the sources investigated with varying rates which ranged from 27% for poultry cages to 80% recorded for fecal samples. Overall prevalence rates based on location ranged from 48% to 76%. Result of the resistance pattern of the Salmonella isolates from the various farms reveal that 62% of total 304 isolates tested were resistant to one or more of the antibiotics used with no significant difference in resistance pattern between the locations. Resistance to ampicillin appears to be the most common and was the highest (43%), followed by trimethoprim-sulphamethozazole (39%) and streptomycin (29%), with cephalexin (9%) and gentamycin (10%) recording the least resistance. Worrisome however is the fact the fluoroquinolones which are comparatively new, expensive and could be drug of choice should there be an outbreak, showed some significant degree of resistance (17%). The findings from this study present a potential health problem and are suggestive of the extent of misuse of drugs in medical and veterinary practice in Nigeria.

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Section: Discussionmentioning

confidence: 99%

Antibiograms of salmonella isolates from poultry farms in Ovia North East local government area, Edo State, Nigeria

Enabulele¹,

Amune²,

Aborisade³

2010

ABJNA

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“…Salmonella, including more than 2,500 different serovars, represents a major cause of foodborne diseases throughout the world [1,2]. In most cases, the infections are associated with ingestion of contaminated food products, particularly those of animal origin such as poultry, eggs, beef, and pork [3,4].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance, class 1 integrons, and horizontal transfer in Salmonella isolated from retail food in Henan, China

Jiang

Zhou

et al. 2014

J Infect Dev Ctries

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Introduction: Salmonellosis remains one of the most frequently occurring foodborne diseases worldwide, especially in developing countries. The increasing prevalence of multidrug resistance among Salmonella isolates from food has been an emerging problem in China. Methodology: In this study, a total of 638 food samples including raw meat, seafood, vegetables, and cooked meat were collected in Henan province of China between July 2007 and August 2008 to determine the prevalence of Salmonella. These isolates were subjected to serotyping, antimicrobial susceptibility, presence of class 1 integrons, and horizontal transfer of integrons. Results: The overall percentage of Salmonella prevalence was 9.7% (n = 62). Among these isolates, S. Anatum and S. Senftenberg were most common, and high rates of antimicrobial resistance were observed to sulfamethoxazole (90.3%), trimethoprim/sulfamethoxazole (87.1%), streptomycin (29.0%), and ciprofloxacin (25.8%). Class 1 integrons were detected in 16.1% of these isolates, and contained gene cassettes dfrA12-aadA2, dfrA1-aadA1, and dfrA1. Three Salmonella isolates could transfer their integrons and resistance genes to Escherichia coli by conjugation. Conclusions: Our findings indicate that the mobile DNA elements could play an important role in the dissemination of resistance determinants among those Salmonella isolates.

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“…The lack of correlation between genotype and the antibiotic resistance profile pattern we detected has previously been documented for different S. enterica serotypes isolated from humans, animals, and food using the same molecular markers we used [6,56,57,58]. This lack of correlation might be partially explained by the fact that both repetitive and RAPD markers are dispersed throughout the bacterial chromosome, while genetic resistance determinants are allocated in restricted genetic regions, or even outside chromosomes, as in the case of plasmids [53].…”

Section: Discussionmentioning

confidence: 48%

Investigation of a food-borne Salmonella Oranienburg outbreak in a Mexican prison

Vázquez-Garcidueñas

Romero-Pérez

Figueroa-Aguilar

et al. 2014

J Infect Dev Ctries

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Introduction: Gastroenteritis outbreaks in prisons represent a public health risk worldwide. Identifying and characterizing the etiological agents of gastroenteritis outbreaks in prisons is important for implementing effective prevention and infection control measures. We present the first studied case of a gastroenteritis outbreak in a Mexican prison. Methodology: Rectal swab samples were obtained from affected inmates. Standard microbiological techniques were used for isolating Salmonella enterica. Isolates were typed by PCR assays of DNA repetitive elements (ERIC, BOX, REP) and RAPD. Antibiotic resistance profiles were performed by the Kirby-Bauer method. Results: S. enterica serotype Oranienburg was responsible for the outbreak affecting 150 inmates. All patients presented diarrhea, and 70% of them also presented vomiting, with no fatal cases. The origin of the outbreak was undetermined due to the difficulty of gathering epidemiological information, but was likely the result of consumption of shrimp broth or a cantaloupe melon beverage. REP, BOX, and ERIC analyses of 26 serotype Oranienburg strains resulted in Simpson discrimination index (D) values of 0, 0.5507, and 0.5661, respectively. The D values from DG93-RAPD analyses and from the combined ERIC-BOX-DG93 markers were 0.7753 and 0.6092, respectively. All strains showed multiresistance to antibiotics. Conclusions: This is the only studied case of a gastroenteritis outbreak in a Mexican prison, and of the first such outbreak caused by serotype Oranienburg. The combined ERIC, BOX, and RAPD markers adequately assessed the genotype diversity of analyzed strains. Penitentiary personnel or inmates involved in outbreaks might spread multiresistant strains outside of the facility.

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Antibiotic resistance and molecular characterization of poultry isolates of Salmonella by RAPD-PCR

Cited by 18 publications

References 13 publications

Antibiograms of salmonella isolates from poultry farms in Ovia North East local government area, Edo State, Nigeria

Antibiograms of salmonella isolates from poultry farms in Ovia North East local government area, Edo State, Nigeria

Antimicrobial resistance, class 1 integrons, and horizontal transfer in Salmonella isolated from retail food in Henan, China

Investigation of a food-borne Salmonella Oranienburg outbreak in a Mexican prison

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