Antibiotic Resistance and Plasmid Profiles of Bacteria Isolated from Abattoir Effluents around Ikpoba River in Benin City, Nigeria

Abstract

Abattoirs are known all over the world to pollute the environment either directly or indirectly from their various processes. The aim of this work is to determine the bacterial load, antibiotic resistance and plasmid profile of resistant bacteria isolated from government and private abattoirs around in Ikpoba River in Benin City. Bacteria and coliform count were done using pour plating method. The total viable bacteria counts ranged 4.2x10 5 ±1.8-1.5xl0 6 ± 0.7 cfu/ml and 1.3x10 6 ± 0.1-3.1 x 10 6 ± 0.5cfu/ml for government and private abattoir respectively, The coliform count ranged from 2.7x10 5 ± 0.1-3.8xl0 5 ±1.4 cfu/ml and 5.5x10 5 ±0.3-9:2xl0 5 ±0.4 cfu/ml for government and private abattoir respectively. Escherichia coli, Streptococcus faecalis, Pseudomonas aeriginosa, Enterobacter aerogenes, Micrococcus luteus, Bacillus subtills and Staphylococcus aureuswere isolated and identified by cultural, morphological and biochemical characteristics. Susceptibility of the microbes was determined. The isolates were resistant to most of the commonly used antibiotics. Plasmid profile of isolates was also determined. The profile study shows that all resistant bacterial isolates harbored plasmid mediated resistance to antibiotics which was confirmed by plasmid curing. Therefore this research showed contamination of abattoirs effluents with antibiotic resistant bacterial isolates. This specially calls for treatment of the wastewater before discharging into the river.

Distribution and Health Risk Assessment of Selected Endocrine Disrupting Chemicals in Two Fish Species Obtained from Choba River in Rivers State, Nigeria

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Antibiotics Resistance Pattern of Coliform Bacteria Isolated from Slaughterhouse Wastewater in Jega Town, Kebbi State, Nigeria

“…The higher resistance of E. coli recorded in this research is worrying as recent literature showed that many of the antimicrobial-resistant strains of E. coli that cause human urinary tract and bloodstream infections appear to have arisen from contaminated meat products (Egwuenu et al, 2018). The finding of this research is in line with the report of Atuanya et al (2018) and Savin et al (2020), who reported the resistance of E. coli isolated from slaughterhouse Effluents to Septrin and Augmentin.…”

Molecular Detection of Antibiotic-Resistant Genes in Pseudomonas aeruginosa from Nonclinical Environment: Public Health Implications in Mthatha, Eastern Cape Province, South Africa

“…Abattoir wastes have the ability to contaminate both surface and groundwater. Discharge from abattoir effluent contaminates the environment by introducing pathogens that can affect land and water qualities, thus endangering human, animal, and aquatic ecosystem's health and constituting a menace to human health and environmental safety [ 13 ]. The possibility of pathogens from abattoir effluent and animal waste reaching or discharging into water bodies and developing resistance to antibiotics in human infection is a concern.…”