Antibiotic-Resistance Genes in Waste Water

Karkman, Antti; Thuy, Thi; Walsh, Fiona; Virta, Marko

doi:10.1016/j.tim.2017.09.005

Cited by 728 publications

(376 citation statements)

References 86 publications

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“…Although the HT-qPCR array used in this study can simultaneously profile 285 ARGs and 10 MGEs of interest in a high-throughput manner (Looft et al, 2012;Su et al, 2015), the need for preselected primer sets limits its use to known genes or to genes with high nucleotide similarity to known ones (Karkman et al, 2018). For example, the relative dominance of the ARG classes or resistance mechanisms detected using the HT-qPCR technique mostly relies on the representation of primer sets on the array.…”

Section: Ubiquitous Args In Forest Biomesmentioning

confidence: 99%

“…However, the annotation of ARGs is still dependent on sets of known verified genes in ARG databases (Gibson et al, 2015). Functional metagenomics can overcome the limitations of HT-qPCR and metagenomic sequencing in detecting mostly known ARGs, but the difficulty in the cloning and expression of environmental DNA in a laboratory host is the main disadvantage of this technique (Karkman et al, 2018). Despite the intrinsic limitations of these methodologies, a combination of HT-qPCR, metagenomics and functional metagenomics is still powerful in the determination of antibiotic resistance in all its dimensions.…”

Section: Ubiquitous Args In Forest Biomesmentioning

confidence: 99%

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Diversity of herbaceous plants and bacterial communities regulates soil resistome across forest biomes

Wang

Singh

et al. 2018

Environmental Microbiology

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Antibiotic resistance is ancient and prevalent in natural ecosystems and evolved long before the utilization of synthetic antibiotics started, but factors influencing the large-scale distribution patterns of natural antibiotic resistance genes (ARGs) remain largely unknown. Here, a large-scale investigation over 4000 km was performed to profile soil ARGs, plant communities and bacterial communities from 300 quadrats across five forest biomes with minimal human impact. We detected diverse and abundant ARGs in forests, including over 160 genes conferring resistance to eight major categories of antibiotics. The diversity of ARGs was strongly and positively correlated with the diversity of bacteria, herbaceous plants and mobile genetic elements (MGEs). The ARG composition was strongly correlated with the taxonomic structure of bacteria and herbs. Consistent with this strong correlation, structural equation modelling demonstrated that the positive effects of bacterial and herb communities on ARG patterns were maintained even when simultaneously accounting for multiple drivers (climate, spatial predictors and edaphic factors). These findings suggest a paradigm that the interactions between aboveground and belowground communities shape the large-scale distribution of soil resistomes, providing new knowledge for tackling the emerging environmental antibiotic resistance.

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Section: Ubiquitous Args In Forest Biomesmentioning

confidence: 99%

Section: Ubiquitous Args In Forest Biomesmentioning

confidence: 99%

Diversity of herbaceous plants and bacterial communities regulates soil resistome across forest biomes

Wang

Singh

et al. 2018

Environmental Microbiology

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“…Antibiotic resistance has been emerging worldwide . Patient behaviour, treatment adherence, leftover antibiotics and drug waste should be identified as potential targets for restraining antibiotic resistance, and these may be of greatest interest in primary care, outpatient setting or for infections with great prevalence . Considering the latter, it is estimated that 50% of the world's population will be infected with Helicobacter pylori .…”

Section: Introductionmentioning

confidence: 99%

Correlation of registered drug packs with Maastricht V/Florence Consensus Report and national treatment guidelines for management of Helicobacter pylori infection

Jukic

Rušić

Vuković

et al. 2019

Basic Clin Pharma Tox

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High prevalence of Helicobacter pylori infection, the complexity for its treatment, poor correlation of registered drug packs or poor patient adherence to the treatment may contribute to antibiotic resistance and healthcare costs. The aim of the present study was to investigate whether registered drug packs are in accordance with European and national guidelines for H pylori eradication with reference to the number of drug units. In this study, we considered treatment options for the management of H pylori infection recommended by the Maastricht V/Florence Consensus Report and by national guidelines in the United Kingdom (UK), Croatia, Italy and Slovenia for adults. Drugs proposed by the guidelines were identified in national drug databases in July of 2019. When considering correlation for 10‐day treatment regimens, drug packs registered in Croatia could not be matched with recommendations for sequential therapies. A number of proposed treatments could not be matched due to small variety of drug packs in Croatia. Drug packs registered in the UK more often matched recommended 14‐day treatment regimens and national guidelines. With reference to European guidelines, 10‐day treatments could more frequently be matched in Italy and in Slovenia. Furthermore, results of this study indicate that there is smaller variety in drug pack sizes registered in Croatia and Slovenia when compared to UK and Italy. Considering poor correlation of drug packs with treatment guidelines for H pylori, adherence to antimicrobial treatment and proper disposal of antimicrobials is warrant. Discussing adherence to antimicrobial treatment with patients should be introduced as a standard of patient care and education.

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“…Anthropogenic activities, including agriculture and animal farming, pathogens treating, and other activities, impact significantly on the evolution of antibiotic resistance (Hiltunen et al ). Most antibiotics administered to humans might have residue in the sewage and urban WWTPs, and contributed to the occurrence and spread of ARGs (Rizzo et al ; Karkman et al ). This is consistent with our data.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Microbiomes and Resistomes in Two Karst Groundwater Sites in Chongqing, China

Zhang¹,

Kuang²,

Xu³

et al. 2019

Groundwater

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Karst groundwater is an important water resource, as it accounts for about 15% of the total landscape of the earth and supplies 20% of potable water worldwide. The antibiotics resistance is an emerging global concern, and antibiotics residual and increase of antibiotic resistance genes represent serious global concerns and emerging pollutants. There is no report on the antibiotic resistance genes in groundwater. To survey resistome and microbiome in karst groundwater, two karst water samples were chosen for metagenome and metatranscriptome study, namely the 37th spring (C) and Dongcao spring (R) in Beibei, Chongqing, China. The two sites differ significantly in sulfur content, geochemical parameters, community structure, antibiotic resistance genes, and mechanisms, and these results may be influenced by anthropogenic activities. Combining with the Antibiotic Resistance Genes Database, three types of resistance genes baca, sul2, sul1 are present in R and C, and ant3ia, ermc, tetpa are also present in R. The number of all resistance genes in R was more than C, and Proteobacteria, Bacteroidetes, Nitrospirae are the main sources of antibiotic resistance genes. In addition, a large number of genes related to antibiotic gene transmission and drug resistance were found in both samples. Karst groundwater is an important source of drinking water and a possible venue for the transmission of microbial antibiotic resistance genes. However, few studies addressed this issue in karst groundwater, despite its widespread and great importance to global ecosystem. Karst groundwater is a reservoir for antibiotic resistant genes, and measures to control these resistant genes are urgently needed.

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Antibiotic-Resistance Genes in Waste Water

Cited by 728 publications

References 86 publications

Diversity of herbaceous plants and bacterial communities regulates soil resistome across forest biomes

Diversity of herbaceous plants and bacterial communities regulates soil resistome across forest biomes

Correlation of registered drug packs with Maastricht V/Florence Consensus Report and national treatment guidelines for management of Helicobacter pylori infection

Comparison of Microbiomes and Resistomes in Two Karst Groundwater Sites in Chongqing, China

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