Antibiotic Resistance in Bacterial Isolates from Commercial  Probiotics Used in Aquaculture

Uma, A.; Rebecca, G.

doi:10.20546/ijcmas.2018.701.210

Cited by 12 publications

(7 citation statements)

References 10 publications

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“…This is very risky for increasing resistance in horizontal control of pathogenic bacteria. The presence of resistant genes in probiotic bacteria is not recommended to be used in aquaculture since its possibility of resistant genes spread in pathogenic bacteria (64). This study obtained isolates of Pseudoalteromonas ganghwensis bacteria tested for sensitivity to several types of antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

Fitriadi¹,

palupi²,

Setyawan³

et al. 2023

IJVS

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Vannamei shrimp culture in intensive and traditional ponds is developing rapidly in Indonesia. The development starts with technology, high density, and feeding systems. It results in very high organic material produced in the aquaculture ponds, and this water quality management is necessarily maintained. This study aims to isolate and identify the molecular of proteolytic bacteria from Vannamei shrimp aquaculture ponds using a different system that is as probiotic agents. This study used a survey method and random sampling technique from traditional and intensive shrimp aquaculture. The results showed that the proportion of proteolytic bacteria in traditional ponds was higher than that in intensive ponds. The screening based on colony morphology and highest activity index obtained 4 bacterial isolates of Vibrio sp. and Pseudoalteromonas white an identity value of 98.23-99.80%. The value of this activity was observed at 7.0 cm, subsequently inhibiting Vibrio sp. by 1.5 cm. The bacteria were also found to develop at pH 7 and a temperature of 28°C adequately. Additionally, the maximum proteolytic activity at a 48-hours incubation indicated their potential relevance as a biotechnological probiotic. Further studies on the potential of Pseudoalteromonas ganghwensis strains found that the bacteria have antimicrobial activity and are sensitive to antibiotics that can be used as aquaculture probiotic agents.

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Section: Discussionmentioning

confidence: 99%

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

Fitriadi¹,

palupi²,

Setyawan³

et al. 2023

IJVS

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“…Accurate tracking of antimicrobial use is a difficult endeavor that often involves non-governmental surveys which depend on the honesty and knowledge of producers, both of which can be unreliable ( Shamsuzzaman and Biswas, 2012 ; Pham et al, 2015 ). Mislabeling of probiotic products in shrimp and other aquaculture has also introduced unintended and antimicrobial resistant bacteria into farms, which could be another explanation for the observed lincomycin resistance ( Noor Uddin et al, 2015 ; Uma and Rebecca, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance profiles of Vibrio spp. and Enterococcus spp. in retail shrimp in Northern California

Hirshfeld,

Lavelle,

Lee

et al. 2023

Front. Microbiol.

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Shrimp is one of the most consumed seafood products globally. Antimicrobial drugs play an integral role in disease mitigation in aquaculture settings, but their prevalent use raises public health concerns on the emergence and spread of antimicrobial resistant microorganisms. Vibrio spp., as the most common causative agents of seafood-borne infections in humans, and Enterococcus spp., as an indicator organism, are focal bacteria of interest for the monitoring of antimicrobial resistance (AMR) in seafood. In this study, 400 samples of retail shrimp were collected from randomly selected grocery stores in the Greater Sacramento, California, area between September 2019 and June 2020. The prevalence of Vibrio spp. and Enterococcus spp. was 60.25% (241/400) and 89.75% (359/400), respectively. Subsamples of Vibrio (n = 110) and Enterococcus (n = 110) isolates were subjected to antimicrobial susceptibility testing (AST). Vibrio isolates had high phenotypic resistance to ampicillin (52/110, 47.27%) and cefoxitin (39/110, 35.45%). Enterococcus were most frequently resistant to lincomycin (106/110, 96.36%), quinupristin-dalfopristin (96/110, 87.27%), ciprofloxacin (93/110, 84.55%), linezolid (86/110, 78.18%), and erythromycin (58/110, 52.73%). For both Vibrio and Enterococcus, no significant associations were observed between multidrug resistance (MDR, resistance to ≥3 drug classes) in isolates from farm raised and wild caught shrimp (p > 0.05) and in isolates of domestic and imported origin (p > 0.05). Whole genome sequencing (WGS) of a subset of Vibrio isolates (n = 42) speciated isolates as primarily V. metschnikovii (24/42; 57.14%) and V. parahaemolyticus (12/42; 28.57%), and detected 27 unique antimicrobial resistance genes (ARGs) across these isolates, most commonly qnrVC6 (19.05%, 8/42), dfrA31 (11.90%, 5/42), dfrA6 (9.5%, 4/42), qnrVC1 (9.5%, 4/42). Additionally, WGS predicted phenotypic resistance in Vibrio isolates with an overall sensitivity of 11.54% and specificity of 96.05%. This study provides insights on the prevalence and distribution of AMR in Vibrio spp. and Enterococcus spp. from retail shrimp in California which are important for food safety and public health and exemplifies the value of surveillance in monitoring the spread of AMR and its genetic determinants.

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“…According to Hernandez (2005) and Cabello (2006), several classes of antimicrobial drugs are commonly used in large quantities in fish farming especially in developing countries including Nigeria where the use of these drugs is not regulated. Similarly, when antimicrobials are mixed with fish feed as growth promoters (Svobodova et al, 2006;Kan and Meijer, 2007), residues may be deposited in the meat, and consumers may inadvertently consume fish containing these residues, which may lead to changes in their microbial flora (Uma and Rebecca, 2018), thus altering the intestinal ecology and favoring the emergence of resistant microflora and drug allergies such as hypersensitivity reactions and other human hazards (Berends et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Assessment of antimicrobial drugs use and their residue in the farmed fish of Kaduna, Nigeria

Ladan¹,

Chukwudi²,

Kabir³

et al. 2021

Glo. J. Fish. Sci.

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The study assessed antimicrobial drug use, pattern, and the occurrence of residues in farmed fish, as well as the level of awareness of drug residues amongst fish farmers in Kaduna State. A total of 300 fish samples from 30 farms in the three senatorial districts of Kaduna State were screened for the presence of antimicrobial drug residues. One hundred questionnaires were administered to fish farmers. Out of the 300 fish samples screened, antimicrobial drug residues were detected in 73.70%. An overall prevalence of 73.70% was obtained, while the prevalence of 63, 74, and 84% was obtained from Kaduna North, Central, and South senatorial districts respectively. From the questionnaire responses, the use of antimicrobial drugs in fish ponds was common (91.86%); with tetracycline (63.41%) being the most frequently used. Self-prescription and administration of drugs by farmers (67.86%) were more in number than those who consult veterinarians for prescription and drug administration. The level of awareness on antimicrobial drug residues in fish and dangers associated with the use and consumption of these residues were very low. Furthermore, the majority of the farmers did not observe antibiotic withdrawal periods before sales of fish to consumers. This study has established a high prevalence of antimicrobial drug residues in farmed fish in the study area. In addition, awareness of the dangers and public health implications of antimicrobial drug residues in fish amongst farmers in Kaduna is low. Therefore, the use of the antimicrobial drug in aquaculture should be regulated and farmers should be encouraged to use immunostimulators or probiotics for disease control and prevention, to safeguard the general populace from the hazards associated with drug residues in fish.

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Antibiotic Resistance in Bacterial Isolates from Commercial Probiotics Used in Aquaculture

Cited by 12 publications

References 10 publications

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

Isolation and molecular identification of proteolytic bacteria from vaname shrimp (Lithopenaeus Vannamei) ponds as probiotic agents

Prevalence and antimicrobial resistance profiles of Vibrio spp. and Enterococcus spp. in retail shrimp in Northern California

Assessment of antimicrobial drugs use and their residue in the farmed fish of Kaduna, Nigeria

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