Antibiotic Resistance of E. Coli Isolates from Broiler Chick's Cecum in Makassar City

Purwanto, Erdi; Marmansari, Dini; Sari, Dwi Kesuma; Hatta, Muhammad

doi:10.20956/jrvi.v3i2.7462

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Previous research was performed in 1988 and found several enteric pathogens such as E. coli, Klebsiella, and Enterobacter has antibiotic resistance to sulphamethoxazole, cephalothin, ampicillin, gentamycin, and chloramphenicol [6]. The further researches were more focused on the livestockrelated animal such as cattle and broiler chicken [7], [8], [9]. Therefore, the aim of this study is to isolate the bacteria from fruit bat Chironax melanocephalus in Gunung Halimun Salak National Park (TNGHS) and analyze the antibiotic resistance profile of the bacterial isolates.…”

Section: Introductionmentioning

confidence: 99%

Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)

Masrukhin¹,

Saputra²,

Rizal³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Antibiotic resistance is one of the most serious health issues in the world which is commonly attributed to the overuse of antibiotics whether in human or animal treatment. The dispersion of antibiotic resistance genes not only found in bacteria from the clinical sample but also found in wildlife animals. Therefore, the aim of this research is to isolate, identify and perform antibiotic resistance assay in bacterial isolates from fruit bat Chironax melanocephalus. Animal samples were taken by using a mist net installed in one of the spots in Gunung Halimun Salak National Park (TNGHS). Bacteria sample was taken using an oral and rectal swab. The bacteria were then isolated in the laboratory by using xylose lactose deoxycholate (XLD) agar medium. There were two bacteria successfully isolated and identified using 16s rDNA amplification. The identification was performed by comparing the 16s rDNA genes to the two online databases i.e BLAST-N in Genebank and 16s-Based ID in the EzBioCloud database. According to the identification results, isolate 4A.1 was identified as Proteus mirabilis while isolate 5.B.2 is identified as Serratia marcescens. Then antibiotic susceptibility test was performed using the disk diffusion method using four different antibiotics disk i.e oxacillin, cefoxitin, amikacin and amoxicillinclavulanic acid. The resistance profile of each bacteria is different between P. mirabilis and S. marcescens in each antibiotic. However, all isolates were found susceptible against amikacin 30 µg.

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Section: Introductionmentioning

confidence: 99%

Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)

Masrukhin¹,

Saputra²,

Rizal³

et al. 2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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“…A previous study conducted in 1988 in Java island, Indonesia showed a 13.3% TC resistance in AMR E. coli isolated from rats [ 8 ]. In recent studies, the prevalence of E. coli with TC resistance accounted for 64–88% in hospitalized patients and 85.3% in broiler chickens in Indonesia [ 16 , 22 , 25 ]. In Indonesia, TC has been widely used in the livestock industry and is also often used for self-medication in humans, which may have led to the increase in the contamination of TC resistant E. coli in the environment [ 8 , 11 , 16 ].…”

mentioning

confidence: 99%

Antimicrobial resistance in <i>Escherichia coli</i> isolated from brown rats and house shrews in markets, Bogor, Indonesia

Huy

Koizumi

Nuradji

et al. 2021

The Journal of Veterinary Medical Science

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The prevalence of antimicrobial resistance (AMR) in small mammals dwelling in the city was used as an indicator of AMR bacteria in the environment. We captured 87 small mammals (79 brown rats and 8 house shrews) in four markets, Bogor, Indonesia in October 2019, and we obtained 20 AMR Escherichia coli ( E. coil ) from 18 brown rats and two house shrews. Of these, eight isolates were determined to be multi-drug resistant (MDR) E. coli , suggesting the potential contamination of AMR E. coli in the markets in Bogor, Indonesia, and that mammals, including humans, are at risk of infection with AMR E. coli from environment.

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Prevalence of multidrug-resistant and extended-spectrum β-lactamase producing Escherichia coli from local and broiler chickens at Cibinong market, West Java, Indonesia

Rizal,

Nurhapsari,

Fauziah

et al. 2024

Vet World

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Background and Aim: Antimicrobial resistance (AMR) is becoming a public health concern. Foodborne pathogens are infectious agents that can be transmitted from animals to humans through food and can become resistant due to misuse and overuse of antibiotics, especially in poultry. This study aimed to detect the prevalence of multidrug-resistant and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from local and broiler chickens at the Cibinong market, West Java, Indonesia. Materials and Methods: A total of 60 cloacal swab samples from 30 local and broiler chickens sold at the Cibinong market in West Java were obtained by random sampling. From these samples, 39 E. coli isolates were obtained after being cultured on eosin methylene blue agar and molecularly identified using polymerase chain reaction (PCR). Six antibiotic disks were used for the antibiotic sensitivity test against E. coli isolates cultured on Mueller-Hinton agar. PCR was performed to detect ESBL genes (blaTEM, blaSHV, and blaCTX-M). Results: A total of 76.47% (39/51) cloacal swab samples were positive for E. coli. All E. coli isolates were sensitive to imipenem (100%), and 38 isolates were sensitive to cefoxitin (FOX) (97.4%). On average, the isolates were sensitive to amoxicillin-clavulanic acid (AMC) (69.2%) and ceftriaxone (CRO) (89.7%). E. coli isolates were occasionally resistant to enrofloxacin (25.64%), followed by gentamicin (20.51%), CRO (10.25%), AMC (7.69%), and FOX (2.56%). The prevalence of E. coli AMR was 10.25% (4/39). All four multidrug-resistant E. coli isolates (blaTEM and blaCTX-M) were confirmed to have the ESBL gene based on PCR. Conclusion: The prevalence of multidrug-resistant and ESBL-producing E. coli is still found, proving that there is still inappropriate use of antibiotics and a need for strict supervision of their use, especially around Cibinong market, West Java. Keywords: antibiotic, chicken, extended-spectrum beta-lactamase, Escherichia coli, multidrug resistance.

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Antibiotic Resistance of E. Coli Isolates from Broiler Chick's Cecum in Makassar City

Cited by 3 publications

References 5 publications

Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)

Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)

Antimicrobial resistance in <i>Escherichia coli</i> isolated from brown rats and house shrews in markets, Bogor, Indonesia

Prevalence of multidrug-resistant and extended-spectrum β-lactamase producing Escherichia coli from local and broiler chickens at Cibinong market, West Java, Indonesia

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