Antibiotic resistance of Enterococcus species isolated from raw foods of animal origin in South West part of Slovakia

Abstract

Kročko M., Čanigová M., Ducková V., Artimová A., Bezeková J., Poston J. (2011): Antibiotic resistance of Enterococcus species isolated from raw foods of animal origin in South West part of Slovakia. Czech J. Food Sci., 29: 654-659.We determined the prevalence and antibiotic resistance of enterococci isolated from raw foods of animal origin. All samples were positive for enterococci. The lowest count of enterococci was found in pork (2.00 log CFU/cm 2 ), while bryndza cheese contained the highest count (4.99 log CFU/g). Among the 349 Enterococcus isolates, 49% were E. faecalis, 29% E. faecium, and 13% Enterococcus spp. Tetracycline and gentamicin resistance was the most common. We found the highest tetracycline resistance levels (91%) in isolates from poultry samples. The isolates from the poultry samples also displayed multidrug resistance to all antibiotics tested. The most common vancomycin-resistant species in poultry and milk was E. faecalis. In contrast, the pork samples contained vancomycin-resistant E. faecium isolates.It is interesting to note that vancomycin resistance in the pork and poultry samples was found only in combination with either four (28%) or all five (14%) of the tested antibiotics. Our results suggest that raw products of animal origin are possible reservoirs of multi-antibiotic resistant enterococci in the food chain.

Antibiotic resistance in vancomycin‐resistant lactic acid bacteria (VRLAB) isolated from foods of animal origin

“…Other studies have shown that E. faecalis and E. faecium constitute the dominant flora in isolates obtained from animal‐originated foods (Cho et al, 2013; Jiménez et al, 2013; Morandi et al, 2015; Valenzuela, Benomar, Abriouel, Cañamero, & Gálvez, 2010; Yogurtcu & Tuncer, 2013). In contrast to our results, vancomycin‐resistant E. faecalis and/or E. faecium strains were isolated from animal‐originated foods in Slovakia (Kročko et al, 2011), Korea (Cho et al, 2013), Turkey (Çetinkaya et al, 2013), Spain (Guerrero‐Ramos et al, 2016), and Nigeria (Igbinosa & Beshiru, 2019). Pediococcus and Weissella can be isolated from animal‐originated foods as well as the commonly‐isolated Enterococcus species.…”

Phenotypic and Genotypic Identification of Vancomycin Resistant Enterococci from Different Sources

“…Resistance to rifampin seems to be widely spread among Enterococci and was the highest percentage between the tested antimicrobials (85.3%). This was similar to the results of Sarra et al [36], while, Kročko et al [30] reported that tetracycline and gentamicin resistance was the most common. Sarra et al [36] found that, none of the tested isolates demonstrated resistance to ampicillin, vancomycin and gentamicin.…”

“…Notably, E. faecalis was the predominant species recovered (42.8%), followed by E. faecium (39.2%), E. casseliflavus (3.5%) and only 4 isolates were not identified to species level (14.2%). Similar results of E. faecalis predominance but with higher percentage (49%) were reported [30]. In addition, Gangurde et al [31] in South West part of Slovakia isolated E. faecalis (60%) in followed by E. faecium 32.2%.…”

“…Herewith, the results revealed that Enterococci percentage in both meat (sausage) and milk sample are high and slightly close to each other as 64.3% and 62%, respectively. On the contrary, Krocko et al [30] revealed lower levels of contamination in meat compared to milk or cheese.…”

“…E. faecium was the predominant genotype in vancomycin resistant isolates but with a higher percentage of 83.5% [39]. Lower percentage of MDR was identified in pork and chicken samples [30].…”