Antibiotic Resistance of Escherichia coli Isolated from Beef and its Related Samples in Techiman Municipality of Ghana

Abstract

In the present study, antibiotic susceptibility test was performed using the disc diffusion method and the results interpreted using the Clinical and Laboratory Standards Institute guidelines. A total of 45 Escherichia coli isolates were screened against nine different antibiotics. Overall, 34.57% of the Escherichia coli isolates were resistant, 7.16% were intermediate and 58.27% were susceptible. Resistance to vancomycin (88.89%) and erythromycin (68.89%) was high. Susceptibility to ciprofloxacin (95.56%), amoxycillin/clavulanic acid (86.67%), suphamethoxazole/ trimethoprim (82.22%) and gentamicin (75.56%) was also high. The Escherichia coli isolates also exhibited 25 antibiotic resistant patterns with the pattern VaE (vancomycin and erythromycin) and VaCCro (vancomycin, chloramphenicol and ceftriaxone) being the commonest (each exhibited by five different isolates). Multiple Antibiotic Resistance index (MAR index) ranged from 0.11-0.78. Resistance to seven (MAR index of 0.78) and five (MAR index of 0.56) different antibiotics was exhibited by 1 and 3 isolates, respectively. Some Escherichia coli isolates from different sources did exhibit the same resistance pattern. This study established the fact that Escherichia coli from meat and it related samples in Techiman Municipality were resistant to some antibiotics. Therefore, the use of antibiotics in the treatment of animals in the Municipality ought to be checked and controlled to prevent more isolates from becoming resistant. To the best of author's knowledge, this is the first report on the antibiotic resistance of Escherichia coli isolated from beef and its related samples in Techiman Municipality of Ghana.

Antibiotic Susceptibility of Escherichia coli Isolated from some Drinking Water Sources in Tamale Metropolis of Ghana

“…The result obtained for the antimicrobial susceptibility test is shown in Table 1. From Table 1, the (Adzitey et al, 2012b;Adzitey, 2015). It is also indicated that in clinical diagnoses patients with intermediate results can be given a higher dosage of antibiotics.…”

“…Res. Bacteriol., 8 (2): 34-40, 2015 Researches in the antibiotic susceptibility of Escherichia coli and other food/waterborne pathogens in Ghana are limited and the few researches available have concentrated more on human isolates (Adzitey, 2015). Saba and Tekpor (2015) in their work determined the antibiotic resistance patterns of Escherichia coli O157: H7 isolated from swimming pools and found that, the Escherichia coli were resistant to ceftriaxone (25%), chloramphenicol (21%), amoxycillin-clavulanic acid (14%), ciprofloxacin (11%) and gentamicin (4%).…”

“…It has been established that antibiotic resistance patterns vary from one region to the other (Subba et al, 2013;Talukdar et al, 2013;Carnot et al, 2014;Saba and Tekpor, 2015). These differences are as a result of variations in time and samples examined, sampling methodology employed and the extent to which antibiotics are used in the various regions (Adzitey, 2015). Antibiotic resistant Escherichia coli is potential source of transmission of resistance to other water borne pathogens.…”

First report of isolation and antibiotic susceptibility pattern of Raoultella electrica from table eggs in Jaipur, India

“…Different resistance patterns were observed against different antibiotics ( Table 3 ). The MAR index of the isolates (which ranged from 0.1 to 0.6) also revealed the contamination level and health risks at the sample collection sites [34] , [35] .…”

Antibiotic resistance of Escherichia coli and Salmonella enterica isolated from cabbage and lettuce samples in Tamale metropolis of Ghana

“…In Ghana, there are fewer reports on antibiotic resistance of foodborne pathogens. The few studies available have also concentrated much on foodborne pathogens isolated from animals and humans (Mills-Robertson et al 2002;Awua-Boateng 2007;Nsoah, 2015;Donkor et al 2012;Adzitey 2015a;Adzitey et al 2015;Andoh et al 2016). However, vegetables in Ghana, including cabbages and lettuces are important sources of resistant foodborne pathogens and should not be ignored.…”