Antibiotic Resistance Patterns of Escherichia coli Isolates from Hospitals in Kumasi, Ghana

Abstract

Nosocomial infections are infections acquired by a patient as a result of treatment in a hospital or healthcare service providing center and symptoms occurs within a short period of hospitalization. The study was to determine the antibiotic resistance patterns of Escherichia coli isolated from Kumasi-South, Tafo and Suntreso Hospitals, Kumasi, Ghana. Total of 600 swabs samples from the hospitals were collected between January and June, 2010. The isolates were identified using morphological and biochemical means. A total of 97 E. coli isolates were obtained from the hospitals. Beds in hospital wards had the highest number of E. coli strains (53.6%), followed by floors (20.6%) while drainages had the least isolates (3.1%). Majority of the E. coli isolates (90.7%) exhibited resistance to ampicillin while 6.2 and 3.1% showed intermediate and sensitive respectively. Co-trimoxazole, 78.4% of the isolates were resistant while 9.3 and 12.4% exhibited intermediate and sensitive responses respectively. E. coli isolates (28.6 to 46.4%) were resistant to gentamicin, ciprofloxacin and ceftriaxone while 14.4 to 47.4% gave intermediate responses. Most isolates (80.4%) exhibited multi-drug resistance. There is a need to observe proper personal hygiene, use of effective disinfectants and proper disposal of contaminated/pathogenic materials in these hospitals to control nosocomial infections.

Restriction Sites Annotation on Klebsiella 16s Ribosomal RNA using Selected Sticky End Cutting Endonucleases

“…The only Klebsiella isolate compared to other isolates had an overall high antibiotic susceptibility but the high susceptibility to Gentamicin (100%) and Ciprofloxacin (100%) is not in agreement with 17.39% and 41.3% respectively of Ullah et al, 2009 and 35.4% and 62.5% respectively of Abdullah et al, 2013. The antibiogram of the 10 E. coli isolates showed low susceptibility of 20% to Ampicillin which is similar to 11.6% reported by Niranjan et al, 2014 and 10.3% reported by Duredoh et al, 2012. However, Susceptibility of Ciprofloxacin and Gentamicin showed a 50% susceptibility which is close to 70% and 53.6% respectively as reported by Duredoh et al, 2012. The antibiogram of Pseudomonas with 9 isolates reveal susceptibility to Streptomycin 3(33%); Ciprofloxacin 6(66%); Gentamicin 4(44%); Nalidixic acid 3(33%); Pefloxacin 7(77%); Augmentin 1(11%); Septrin 6(66%); Ampicillin 2(22%); Cephalosporin 2(22%) and Ofloxacin 7(77%).…”

“…However, Susceptibility of Ciprofloxacin and Gentamicin showed a 50% susceptibility which is close to 70% and 53.6% respectively as reported by Duredoh et al, 2012. The antibiogram of Pseudomonas with 9 isolates reveal susceptibility to Streptomycin 3(33%); Ciprofloxacin 6(66%); Gentamicin 4(44%); Nalidixic acid 3(33%); Pefloxacin 7(77%); Augmentin 1(11%); Septrin 6(66%); Ampicillin 2(22%); Cephalosporin 2(22%) and Ofloxacin 7(77%). The percentage susceptibility is in agreement with studies by Golshani et al, 2012 with Gentamicin having 40%.…”

Antibiotic Resistance of Escherichia coli Isolated from Beef and its Related Samples in Techiman Municipality of Ghana

“…George et al (2012) determined the antibiotic resistance patterns of Escherichia coli isolated from patients in selected hospitals in Kumasi, Ghana and reported that Escherichia coli isolates (28.6-46.4%) were resistant to gentamicin, ciprofloxacin and ceftriaxone while 14.4-47.4% gave intermediate responses. This study found 13.33% resistance to gentamicin, 2.22% resistance to ciprofloxacin and 26.67% Table 1 Table Cro 1 (Namboodiri et al, 2011).…”

Antibiotic Practices and Factors Influencing the Use of Antibiotics in Selected Poultry Farms in Ghana

“…It has been reported that the misuse of antibiotics in food-animal production is one of the most important factors contributing to the global surge and spread in antibiotic resistance [12]. Several studies have reported high level of antibiotic resistant bacteria isolated from patients visiting healthcare facilities in Ghana [13][14][15][16][17] and even among the healthy populace [18]. These reports show an increase in antibiotic resistance in both commensals and pathogenic bacteria and this raises a concern to public health system.…”