Antibiotic Resistance Profile, Outer Membrane Proteins, Virulence Factors and Genome Sequence Analysis Reveal Clinical Isolates of Enterobacter Are Potential Pathogens Compared to Environmental Isolates

Mishra, Mitali; Panda, Sasmita; Barik, Susmita; Sarkar, Arup; Singh, Deepika; Mohapatra, Harapriya

doi:10.3389/fcimb.2020.00054

Cited by 27 publications

(20 citation statements)

References 52 publications

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“…It would be expected there would be more and/or more potent virulence factors from clinical isolates compared to environmental isolates. For example, as corroborated by whole genome sequencing, in a recent study, it was suggested that there was limited pathogenic potential of environmental Enterobacter species isolates compared to clinical isolates (Mishra et al 2020). In our study, the only difference between the three clinical isolates and the two environmental isolates was that environmental isolate CGG3 does not have siderophores.…”

Section: Putative Virulence Factorssupporting

confidence: 61%

Searching for putative virulence factors in the genomes of Shewanella indica and Shewanella algae

Tamez

McLaughlin

et al. 2020

Arch Microbiol

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Bacterial pathogens are a major threat to both humans and animals worldwide. It is crucial to understand the mechanisms of various disease processes at the molecular level. Shewanella species are widespread in the environment and some are considered as emerging opportunistic human and marine mammal pathogens. In this study, putative virulence factors on the genome of Shewanella indica BW, a bacterium isolated from the Bryde's whale (Balaenoptera edeni), were determined. Additionally, for comparative purposes, putative virulence factors from two other S. indica and ten S. algae strains were also determined using the Pathosystems Resource Integration Center (PATRIC) pipeline. We confirmed the presence of previously reported virulence factors and we are proposing several new candidate virulence factors. Interestingly, the putative virulence factors were very similar between the two species with the exception of microbial collagenase which was present in all S. algae genomes, but absent in all S. indica genomes.

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Section: Putative Virulence Factorssupporting

confidence: 61%

Searching for putative virulence factors in the genomes of Shewanella indica and Shewanella algae

Tamez

McLaughlin

et al. 2020

Arch Microbiol

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“…This explains why ESR systems are involved or contribute in the sensing and transmission of internal signal that activate the genetic cascades controlling the envelope permeability and transports. Consequently, the TCS systems have been proposed as new target for original antibacterial agents in order to impair the bacterial adaptation and virulence [ 86 , 119 , 133 , 134 , 135 ]. In the same way, targeting sRNAs is currently being considered in view of their central implication in the regulatory network leading to antibiotic resistance [ 88 , 90 ].…”

Section: Discussionmentioning

confidence: 99%

An Intertwined Network of Regulation Controls Membrane Permeability Including Drug Influx and Efflux in Enterobacteriaceae

et al. 2020

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The transport of small molecules across membranes is a pivotal step for controlling the drug concentration into the bacterial cell and it efficiently contributes to the antibiotic susceptibility in Enterobacteriaceae. Two types of membrane transports, passive and active, usually represented by porins and efflux pumps, are involved in this process. Importantly, the expression of these transporters and channels are modulated by an armamentarium of tangled regulatory systems. Among them, Helix-turn-Helix (HTH) family regulators (including the AraC/XylS family) and the two-component systems (TCS) play a key role in bacterial adaptation to environmental stresses and can manage a decrease of porin expression associated with an increase of efflux transporters expression. In the present review, we highlight some recent genetic and functional studies that have substantially contributed to our better understanding of the sophisticated mechanisms controlling the transport of small solutes (antibiotics) across the membrane of Enterobacteriaceae. This information is discussed, taking into account the worrying context of clinical antibiotic resistance and fitness of bacterial pathogens. The localization and relevance of mutations identified in the respective regulation cascades in clinical resistant strains are discussed. The possible way to bypass the membrane/transport barriers is described in the perspective of developing new therapeutic targets to combat bacterial resistance.

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“…In Gram-negative bacteria, the production of chromosomal AmpC enzymes was lower compared to plasmid-encoded AmpC enzymes; however, this product can be enhanced by the induction of some β-lactams, such as cefoxitin. E. coli and K. pneumoniae strains harbouring AmpC β-lactamases have the ability to lose porin Omp-K35 from their outer membrane and show resistance against cephamycin because this porin permits water soluble β-lactams to passthrough and gain access into the cells [63]. Recent studies found that more than twenty plasmid-expressed AmpC β-lactamases exist.…”

Section: Ampc β-Lactamasesmentioning

confidence: 99%

Genetic basis of molecular mechanisms in β-lactam resistant gram-negative bacteria

Hussain

Aqib

Seleem

et al. 2021

Microbial Pathogenesis

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Antibiotic-resistant bacteria are considered one of the major global threats to human and animal health. The most harmful of the resistant bacteria are β-lactamases producing Gram-negative species (β-lactamases). β-lactamases constitute a paradigm shift in the evolution of antibiotic resistance. Therefore, it is imperative to present a comprehensive review of the mechanisms responsible for the development of antimicrobial resistance. Resistance due to β-lactamases develops through to a variety of mechanisms, and the number of resistant genes is involved that can be transferred between bacteria, mostly via plasmids. Over time, these new molecular based resistance mechanisms have been progressively disclosed. The present review article provides information on the recent findings regarding the molecular mechanisms of resistance to β-lactams in Gram-negative bacteria, including CTX-M-type ESBLs with methylase activity, plasmids harbouring phages with β-lactam resistance genes, the co-presence of β-lactam resistant genes of unique combinations and the presence of β-lactam and non-β-lactam antibiotic-resistant

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Antibiotic Resistance Profile, Outer Membrane Proteins, Virulence Factors and Genome Sequence Analysis Reveal Clinical Isolates of Enterobacter Are Potential Pathogens Compared to Environmental Isolates

Cited by 27 publications

References 52 publications

Searching for putative virulence factors in the genomes of Shewanella indica and Shewanella algae

Searching for putative virulence factors in the genomes of Shewanella indica and Shewanella algae

An Intertwined Network of Regulation Controls Membrane Permeability Including Drug Influx and Efflux in Enterobacteriaceae

Genetic basis of molecular mechanisms in β-lactam resistant gram-negative bacteria

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