Antibiotic resistance profiles of vancomycin resistant enterococci in chicken meat samples

Abstract

Antibiotic resistance is one of the serious threats to global public health and food safety today. Acquired antibiotic resistance in microorganisms arises from prevalent use of antibiotics for human and animal medicine. Owing to the fact that Vancomycin Resistant Enteroccocci (VRE) is a vital problem for public health, determination of the antibiotic resistance profiles of Enterococcus spp. isolates have crucial importance as a part of the farm to fork food safety. In the study, 120 retail chicken meat samples were analyzed, and 36 (30%) of the samples were detected as Enterococcus spp. positive. According to the results, the most prevalent species was E. faecalis with a rate of 44.4% (16/36), followed by 27.8% (10/36) E. faecium, 11.1% (4/36) E. durans, 2.8% (1/36) E. gallinarum and 2.8% (1/36) E. casseliflavus. Antibiotic resistance profiles of the verified Enterococcus spp. isolates were determined with disc diffusion method in terms of eight different antibiotics. Among the Enterococcus spp. isolates, 20 (55.5%) isolates were phenotypically resistant to vancomycin, 6 isolates (16.7%) were detected as vanA positive, 3 isolates (8.3%) were detected as vanB positive, and one isolate (5%) showed high resistance to vancomycin (MIC >256 µg/ml). Even though the observed percentages are low, the observed resistance patterns are still of concern for public health.

Tavuk Etlerinde Enterococcus spp. Prevelansı, Direnç Profilleri, VanA ve VanB Direnç Genlerinin Varlığı

“…Among the 100 chicken meat samples, 27% were determined to be Enterococcus spp. positive, which is close to the value of 28.6% that was reported by Pesavento et al (2014) and the value of 30% that was reported by Onaran et al (2019). Yüksel et al (2013), Kilonzo-Nthenge et al (2015), Donado-Godoy et al (2015), Şentürk (2017), Kim et al (2018), andSanlibaba et al (2018) reported higher prevalence values of 43%, 82.2%, 94%, 91.66%, 77.7%, and 79.50%, respectively, while Bayram et al (2011) and Gousia et al (2015) reported lower prevalence values of 12.5% and 21.7%, respectively.…”

“…The antibiotic sensitivity of the E. faecalis and E. faecium isolates differ depending on geographical conditions. Various studies have been conducted in Turkey (Kasimoglu-Dogru et al 2010, Onaran et al 2019Gökmen and Ektik, 2022), Canada (Aslam et al 2012), Amerika-Tennessee (Kilonzo-Nthenge et al 2015), Northwest Greece (Gousia et al 2015), Colombia (Donado-Godoy et al 2015), and South Korea (Kim et al 2018;Kim et al 2019). In the present study, 40.3% of the isolates were resistant to erythromycin and 71.64% of the isolates exhibited resistance to tetracycline.…”

“…In another study, 93.5% of the E. faecium isolates that were obtained from different foods and phenotypically resistant to vancomycin had the VanA gene while 29% had the VanB2,3 gene; the researchers did not detect VanA, VanB, or VanB2,3 in the E. faecalis isolates (Gousia et al 2015). Onaran et al (2019) identified the VanA gene in 16.7% of the Enterococcus spp. isolates and the VanB gene in 8.3% of the isolates.…”

Isolation and antimicrobial resistance of vancomycin resistant Enterococcus spp. (VRE) and methicillin-resistant S. aureus (MRSA) on beef and chicken meat, and workers hands from slaughterhouses and retail shops in Turkey

“…Absence of the vanA gene in phenotypically resistant isolates were in aggrement with several studies (Gousia et al, 2015;Raafat et al, 2016;El-Tawab et al, 2019;Onaran et al, 2019). In this context, Gousia et al (2015) in Greece found that eight out of 30 E. faecium and one E. faecalis vancomycin-resistant isolates did not harbour the vanA, Raafat et al (2016) reported that only one of the 10 vancomycin-resistant isolates was vanA-positive in foods of animal origin, El-Tawab et al (2019) reported that vanA was detected in three out of nine vancomycin-resistant strains isolated from milk and milk products in Egypt and in a recent study by Onaran et al (2019) with the absence of the vanA gene in 14 of 20 phenotypically positive VRE strains. Vancomycin resistance is known to be mediated by several van types as van A, van B, van C, van D, van E etc.…”

Study on Prevalence and Antibiotic Profiling of Enterococcus Spp. from Chicken Meat

“…On the other hand, Rozanska et al (2015) reported moderate prevalence rate (49%) of Enterococcus spp. However, a very low prevalence rate of 30% was reported by Onaran et al (2019), which may be due to good hygienic practices followed in sampling.…”