2019
DOI: 10.3390/microorganisms7080251
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Antibiotic Resistance, Virulence Factors, Phenotyping, and Genotyping of E. coli Isolated from the Feces of Healthy Subjects

Abstract: Escherichia coli may innocuously colonize the intestine of healthy subjects or may instigate infections in the gut or in other districts. This study investigated intestinal E. coli isolated from 20 healthy adults. Fifty-one strains were genotyped by molecular fingerprinting and analyzed for genetic and phenotypic traits, encompassing the profile of antibiotic resistance, biofilm production, the presence of surface structures (such as curli and cellulose), and their performance as recipients in conjugation expe… Show more

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Cited by 39 publications
(33 citation statements)
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“…The selective differential medium HiCrome Coliform Agar (HCCA) was utilized to count and isolate E. coli [5] and NECE from fecal samples of 20 healthy adults. Total counts in HCCA ranged from 4.6 × 10 5 to 2.2 × 10 8 cfu/g ( Figure 1).…”
Section: Counting and Isolation Of Necementioning
confidence: 99%
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“…The selective differential medium HiCrome Coliform Agar (HCCA) was utilized to count and isolate E. coli [5] and NECE from fecal samples of 20 healthy adults. Total counts in HCCA ranged from 4.6 × 10 5 to 2.2 × 10 8 cfu/g ( Figure 1).…”
Section: Counting and Isolation Of Necementioning
confidence: 99%
“…The strains were screened as recipients in conjugation experiments with the donor E. coli N4i pOX38:Cm (N4i: EcN immE7 Gmr; pOX38:Cm: Tra+ RepFIA+ Cmr) [5]. The donor and the recipient strains were cultured and put in contact onto LB plates under the conditions described in [5]. HCCA and HCCA with 20 µg/mL chloramphenicol were utilized to differentiate recipient, transconjugant, and donor colonies [5].…”
Section: Solid Mating Conjugation Experimentsmentioning
confidence: 99%
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