Antibiotic Resistance, Virulence Gene, and Molecular Profiles of Shiga Toxin-Producing
            <i>Escherichia coli</i>
            Isolates from Diverse Sources in Calcutta, India

Khan, Asis; Das, Sanjukta; Ramamurthy, T.; Sikdar, A.; Khanam, Jasmina; Yamasaki, Shinji; Takeda, Yoshifumi; Nair, G. Balakrish

doi:10.1128/jcm.40.6.2009-2015.2002

Cited by 100 publications

(102 citation statements)

References 40 publications

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“…Thus continued surveillance of emerging antimicrobial resistance among zoonotic food-borne pathogens, including STEC is required to ensure public health protection. Although STEC O157: H7 had been considered sensitive to many antibiotics (24), recent results demonstrated resistance of STEC O157:H7, and in special the non-O157 STEC strains (9,12). Results of antimicrobial susceptibility testing (Table 2) showed high resistance to tetracycline, cephalothin, amoxicillin and streptomycin.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Rodolpho

Marin

2007

Braz. J. Microbiol.

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Shiga toxigenic Escherichia coli (STEC) has been implicated as the cause of several human diseases. Samples (ground beef, grinding-machines and human hands) from 23 butcheries were assayed for E. coli using standard microbiological methods, and 287 isolates were submitted to polymerase chain reaction for the detection of stx 1, stx 2 and eae genes. Four STEC isolates were recovered, two from ground beef and two from grinding-machines; all harbored the stx 2 gene and were negative for the eae gene. All E. coli isolates including the four STEC were screened for antibiotic resistance. High levels of resistance against several antimicrobial agents were detected; those most commonly observed were to tetracycline (76.6%), amoxicillin (64.1%) and cephalothin (58.8%). Such high levels of antimicrobial resistance highlight the need for a more rational use of these agents in cattle.

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Section: Discussionmentioning

confidence: 99%

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Rodolpho

Marin

2007

Braz. J. Microbiol.

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“…26 Specifically, the strains were: Clostridium perfringens CECT 376, Clostridium difficile CECT 531, Shigella ENT CBD8, enterotoxigenic E. coli (ETEC) CECT 685, enteropathogenic E. coli (EPEC) CECT 729, and Campylobacter jejuni CECT 7572. The primers used were: C. perfringens (16S rDNA) F: ATG CAA GTC GAG CGA (G/T)G, R: TAT GCG GTA TTA ATC T(C/T)C CTT T 27 ; C. difficile (16S rDNA) F: TTG AGC GAT TTA CTT CGG TAA AGA, R: CCA TCC TGT ACT GGC TCA CCT 27 ; Shigella group ( ipaH gene coding for invasion plasmid antigen H) F: GTT CCT TGA CCG CCT TTC CGA TAC, R: CAT TTC CTT CAC GGC AGT GGA 28 ; ETEC ( elt gene coding for thermolabile toxin) F: GCG ACA AAT TAT ACC GTG CT, R: CCG AAT TCT GTT ATA TAT ATG T 29 ; EPEC ( eae gene coding for adhesin) F: AAA CAG GTG AAA CTG TTG CC, R: CTC TGC AGA TTA ACC CTC TGC 30 ; C. jejuni ( hip gene coding for the hippuricase enzyme) F: GAA GAG GGT TTG GGT GGT G, R: AGC TAG CTT CGC ATA ATA ACT TG. 31 …”

Section: Methodsmentioning

confidence: 99%

Increased prevalence of pathogenic bacteria in the gut microbiota of infants at risk of developing celiac disease: The PROFICEL study

et al. 2018

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Celiac disease (CD) is an immune-mediated enteropathy involving genetic and environmental factors, whose interaction influences disease risk. The intestinal microbiota, including viruses and bacteria, could play a role in the pathological process leading to gluten intolerance. In this study, we investigated the prevalence of pathogens in the intestinal microbiota of infants at familial risk of developing CD. We included 127 full-term newborns with at least one first-degree relative with CD. Infants were classified according to milk-feeding practice (breastfeeding or formula feeding) and HLA-DQ genotype (low, intermediate or high genetic risk). The prevalence of pathogenic bacteria and viruses was assessed in the faeces of the infants at 7 days, 1 month and 4 months of age. The prevalence of Clostridium perfringens was higher in formula-fed infants than in breast-fed over the study period, and that of C. difficile at 4 months. Among breastfed infants, a higher prevalence of enterotoxigenic E. coli (ETEC) was found in infants with the highest genetic risk compared either to those with a low or intermediate risk. Among formula-fed infants, a higher prevalence of ETEC was also found in infants with a high genetic risk compared to those of intermediate risk. Our results show that specific factors, such as formula feeding and the HLA-DQ2 genotype, previously linked to a higher risk of developing CD, influence the presence of pathogenic bacteria differently in the intestinal microbiota in early life. Further studies are warranted to establish whether these associations are related to CD onset later in life.

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“…Conversely, none of the strains carried the gene in the current study. This variation was associated with geographical changes as stated in previous studies [17,19].…”

Section: Discussionmentioning

confidence: 76%

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

2014

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Cow mastitis caused by Escherichia coli (E. coli) exhibits various local and systemic clinical signs at varying degrees of severity. The aim this study was to elucidate the virulence properties, antibiotic resistance and phylogenetics of 56 E. coli strains. Of all the studied strains, 12 were positive for hemolytic properties and 38 were positive for biofi lm production. Additionally, 55 of the strains were positive for multiple resistances in bacteriological tests. PCR analysis revealed that 42 strains carried the traT gene, 20 strains had the shiga toxin gene (stx1-stx2), and 8 strains carried the intimin gene (eae), but all strains were negative for aerobactin gene (aer). All strains encoding shiga toxin genes were also positive for stx1, but only 4 strains were positive for stx2. There were no signifi cant differences in virulence genes between antibiotic-resistant and antibiotic-susceptible strains. The random amplifi ed polymorphic DNA-polymerase chain reaction patterns revealed the existence of 13 main groups with 4 subgroups of E. coli. In this study, E. coli strains causing intramammary infections and originating from various sources might show resistance against common antibiotics. Pathogenity of E. coli that cause clinical mastitis, and prognosis of the infection might be predicted by obtaining the traT gene. Additionally, antibiotic resistance should be investigated at the genomic level to detect the relationship between virulence factors and antibiotic resistance. In fi eld conditions, development of antibiotic resistance is the main cause of mastitis treatment failure. Thus, antibiotic resistance profi les in herds should be monitored and effective antibiotics should be administered.

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Antibiotic Resistance, Virulence Gene, and Molecular Profiles of Shiga Toxin-Producing Escherichia coli Isolates from Diverse Sources in Calcutta, India

Cited by 100 publications

References 40 publications

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil

Increased prevalence of pathogenic bacteria in the gut microbiota of infants at risk of developing celiac disease: The PROFICEL study

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

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