Antibiotic resistant Esherichia coli strains from seafood and its susceptibility to seaweed extracts

Shanmugam, Kumaran; Deivasigamani, B.; Alagappan, Kumarappan; Sakthivel, Mannikam; Karthikeyan, R.

doi:10.1016/s1995-7645(11)60013-8

Cited by 24 publications

(17 citation statements)

References 14 publications

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“…Differences were observed relating source of the sampled Nile tilapia and antimicrobial resistance profiles (Table 2). Generally, fish from wild capture in Lake Victoria had bacteria that were 100% resistant to AMP, ERY, TET and SXT, higher than that reported in tilapia (Rocha et al, 2014) and other fish species elsewhere (Kumaran et al, 2010;Bolarinwa et al, 2011;Ryu et al, 2012;Musefiu and Olasunkanmi, 2015). The observed AMR levels elsewhere could be explained by the fact that different levels of application of antibiotics in aquaculture, which is not documented in Uganda.…”

Section: Resultsmentioning

confidence: 80%

Antimicrobial resistance of Escherichia coli found in intestinal tract of Oreochromis niloticus

Kikomeko¹

2016

Uganda Journal of Agricultural Sciences

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Antimicrobial resistance (AMR) is an emerging public health problem worldwide. Agricultural practices have been suggested as one of the main contributors to the emergence and transmission of AMR worldwide. Aquatic animals like fish have an important role to play in the emergence and transmission of AMR strains. Escherichia coli is commensal in gastrointestinal tracts of many animals including fish, and is an indicator organism and opportunistic pathogen. It has been listed as one of the important bacteria involved in the emergence and spread of antimicrobial resistance factors. The objective of this study was to investigate the AMR patterns of E. coli isolated from the intestinal tract of fish within the Lake Victoria basin. A total of 60 Oreochromis niloticus fish were sampled from cages, wild and earthen ponds. Distal gastrointestinal tract was recovered, crushed and streaked on violet red bile agar, incubated at 37 O C for 24 hours. The isolates confirmed biochemically as E. coli were screened for antibiotic susceptibility against 10 commonly used antibiotics using disc diffusion method. Eighty three percent of the sampled fish were positive for E. coli. Generally, isolates were susceptible to ciprofloxacin, gentamicin, cephalexin, ceftriaxone and nitrofurantoin (100%); tetracycline, sulfamethoxazole-trimethoprim (81.8%) and nalidixic acid (72.7%). However, most of them were resistant to ampicillin and erythromycin (72.7%). The results show that fish are reservoirs of E. coli, which is resistant to some antibiotics. The factors responsible for the observed resistance patterns need to be followed up with more research. Good aquaculture practices, surveillance and monitoring of resistant bacteria will help in understanding the problem of AMR.

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Section: Resultsmentioning

confidence: 80%

Antimicrobial resistance of Escherichia coli found in intestinal tract of Oreochromis niloticus

Kikomeko¹

2016

Uganda Journal of Agricultural Sciences

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“…In one study, >80% of E. coli isolated from an estuary in Kochi, India, were resistant to multiple antibiotics—such as streptomycin, tetracycline, vancomycin, novobiocin, kanamycin, and oxytetracycline—and these included pathogenic groups such as STEC, ETEC, and EPEC [ 35 ]. A recent study [ 33 ] has found high levels of ampicillin and ciprofloxacin resistance in E. coli strains from seafood in India. Although limited numbers of samples from different fish species were analyzed in our study, the results clearly point to the contamination of seafood with multiple antibiotic-resistant, ESBL + enterobacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Amplification of ESBL genes was performed using previously published primers and protocols for bla CTX [ 30 ], bla SHV [ 31 ], bla TEM [ 32 ], and bla NDM genes [ 33 , 34 ]. Bacterial DNA was extracted using Wizard DNA kit (Promega, Madison, WI, USA) was used as template for PCR amplification in a Hybaid thermal cycler (Thermo Fisher Scientific Inc., Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Multiple Antibiotic-Resistant, Extended Spectrum-β-Lactamase (ESBL)-Producing Enterobacteria in Fresh Seafood

et al. 2017

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Members of the family Enterobacteriaceae include several human pathogens that can be acquired through contaminated food and water. In this study, the incidence of extended spectrum β-lactamase (ESBL)-producing enterobacteria was investigated in fresh seafood sold in retail markets. The ESBL-positive phenotype was detected in 169 (78.60%) isolates, with Escherichia coli being the predominant species (53), followed by Klebsiella oxytoca (27), and K. pneumoniae (23). More than 90% of the isolates were resistant to third generation cephalosporins, cefotaxime, ceftazidime, and cefpodoxime. Sixty-five percent of the isolates were resistant to the monobactam drug aztreonam, 40.82% to ertapenem, and 31.36% to meropenem. Resistance to at least five antibiotics was observed in 38.46% of the isolates. Polymerase Chain Reaction (PCR) analysis of ESBL-encoding genes detected blaCTX, blaSHV, and blaTEM genes in 76.92%, 63.3%, and 44.37% of the isolates, respectively. Multiple ESBL genes were detected in majority of the isolates. The recently discovered New Delhi metallo-β-lactamase gene (blaNDM-1) was detected in two ESBL+ isolates. Our study shows that secondary contamination of fresh seafood with enteric bacteria resistant to multiple antibiotics may implicate seafood as a potential carrier of antibiotic resistant bacteria and emphasizes an urgent need to prevent environmental contamination and dissemination of such bacteria.

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“…The studies revealed that ethanol extracts of G. fisheri had immunostimulant and antimicrobial activity that could protect P. monodon against V. harveyi. Kumaran, Deivasigamani, Alagappan, Sakthivel, and Karthikeyan (2010) studied the susceptibility of E. coli in seafood obtained from Cuddalore and Parangipettai fish landing centers against red alga Kappaphycus alvarezii and brown alga Padina boergessenii extracts as sulfated polysaccharides and polyphenols, respectively. The polyphenol and sulfated polysaccharides showed promising inhibitory response against all antimicrobial resistant E. coli strains and in particular the inhibitory response of ampicillin resistant E. coli.…”

Section: Addition To Food As An Antimicrobialmentioning

confidence: 99%

Recent developments in the application of seaweeds or seaweed extracts as a means for enhancing the safety and quality attributes of foods

Gupta¹,

Abu‐Ghannam²

2011

Innovative Food Science & Emerging Technologies

318

207

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Antibiotic resistant Esherichia coli strains from seafood and its susceptibility to seaweed extracts

Cited by 24 publications

References 14 publications

Antimicrobial resistance of Escherichia coli found in intestinal tract of Oreochromis niloticus

Antimicrobial resistance of Escherichia coli found in intestinal tract of Oreochromis niloticus

Multiple Antibiotic-Resistant, Extended Spectrum-β-Lactamase (ESBL)-Producing Enterobacteria in Fresh Seafood

Recent developments in the application of seaweeds or seaweed extracts as a means for enhancing the safety and quality attributes of foods

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