Antibiotic-resistant ST38, ST131 and ST405 strains are the leading uropathogenic<i>Escherichia coli</i>clones in Riyadh, Saudi Arabia

Alghoribi, Majed F.; Gibreel, Tarek M.; Farnham, Garry; Johani, Sameera M. Al; Balkhy, Hanan H.; Upton, Mathew

doi:10.1093/jac/dkv188

Cited by 81 publications

(80 citation statements)

References 21 publications

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“…These STs are associated with bacteremia and urinary tract infection in humans (Adams-Sapper et al, 2012; Alghoribi et al, 2015; Hertz et al, 2016). The MLVA-profiles of the bivalve E. coli isolates displayed a seemingly high degree of diversity ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Grevskott¹,

Svanevik²,

Sunde

et al. 2017

Front. Microbiol.

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The mechanisms for the development and spread of antibacterial resistance (ABR) in bacteria residing in environmental compartments, including the marine environment, are far from understood. The objective of this study was to examine the ABR rates in Escherichia coli and other Enterobacteriaceae isolates obtained from marine bivalve mollusks collected along the Norwegian coast during a period from October 2014 to November 2015. A total of 549 bivalve samples were examined by a five times three tube most probable number method for enumeration of E. coli in bivalves resulting in 199 isolates from the positive samples. These isolates were identified by biochemical reactions and matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry, showing that 90% were E. coli, while the remaining were species within the genera Klebsiella, Citrobacter, and Enterobacter. All 199 isolates recovered were susceptibility tested following the European Committee on Antimicrobial Susceptibility Testing disk diffusion method. In total, 75 of 199 (38%) isolates showed resistance to at least one antibacterial agent, while multidrug-resistance were seen in 9 (5%) isolates. One isolate conferred resistance toward 15 antibacterial agents. Among the 75 resistant isolates, resistance toward extended-spectrum penicillins (83%), aminoglycosides (16%), trimethoprim (13%), sulfonamides (11%), tetracyclines (8%), third-generation cephalosporins (7%), amphenicols (5%), nitrofurans (5%), and quinolones (5%), were observed. Whole-genome sequencing on a selection of 10 E. coli isolates identified the genes responsible for resistance, including blaCTX-M genes. To indicate the potential for horizontal gene transfer, conjugation experiments were performed on the same selected isolates. Conjugative transfer of resistance was observed for six of the 10 E. coli isolates. In order to compare E. coli isolates from bivalves with clinical strains, multiple-locus variable number tandem repeats analysis (MLVA) was applied on a selection of 30 resistant E. coli isolates. The MLVA-profiles were associated with community-acquired E. coli strains causing bacteremia. Our study indicates that bivalves represent an important tool for monitoring antibacterial resistant E. coli and other members of the Enterobacteriaceae family in the coastal environment.

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Section: Discussionmentioning

confidence: 99%

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Grevskott¹,

Svanevik²,

Sunde

et al. 2017

Front. Microbiol.

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“…; Alghoribi et al . ; Day et al . ), were also identified among healthy chickens and cattle in Tunisia and Nigeria respectively.…”

Section: Esbl Plasmid‐mediated Ampc and Carbapenemase‐producing E Cmentioning

confidence: 99%

Antibiotic resistance in Escherichia coli in husbandry animals: the African perspective

Alonso

Zarazaga

Sallem

et al. 2017

Letters in Applied Microbiology

138

106

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In the last few years, different surveillances have been published in Africa, especially in northern countries, regarding antimicrobial resistance among husbandry animals. Information is still scarce, but the available data show a worrying picture. Although the highest resistance rates have been described against tetracycline, penicillins and sulphonamides, prevalence of plasmidmediated quinolone resistance genes and extended spectrum b-lactamase (ESBL) are being increasingly reported. Among ESBLs, the CTX-M-1 group was dominant in most African surveys. Within this group, CTX-M-15 was the main variant both in animals and humans, except in Tunisia where CTX-M-1 was more frequently detected among Escherichia coli from poultry. Certain bla CTX-M-15 -harbouring clones (ST131/B2 or ST405/D) are mainly identified in humans, but they have also been reported in livestock species from Tanzania, Nigeria or Tunisia. Moreover, several reports suggest an inter-host circulation of specific plasmids (e.g. bla CTX-M-1 -carrying IncI1/ST3 in Tunisia, IncY-and Incuntypeable replicons co-harbouring qnrS1 and bla CTX-M-15 in Tanzania and the worldwide distributed bla CTX-M-15 -carrying IncF-type plasmids). International trade of poultry meat seems to have contributed to the spread of other ESBL variants, such as CTX-M-14, and clones. Furthermore, first descriptions of OXA-48-and OXA-181-producing E. coli have been recently documented in cattle from Egypt, and the emergent plasmid-mediated colistin resistance mcr-1 gene has been also identified in chickens from Algeria, Tunisia and South Africa. These data reflect the urgent need of a larger regulation in the use of veterinary drugs and the implementation of surveillance programmes in order to decelerate the advance of antimicrobial resistance in this continent.

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“…Moreover, whereas strain MCR1_NJ was susceptible to various antibiotics, including gentamicin and trimethoprim-sulfamethoxazole, studies of ST405 strains from multiple countries suggest that they are typically resistant to these as well (20). Worrisomely, ST405 has been frequently associated with community onset urinary tract infections (23–25). Dissemination of mcr-1 within this global lineage may therefore contribute to further spread of polymyxin resistance within ESBL- and carbapenemase-producing E. coli (and other Enterobacteriaceae ) strains.…”

Section: Observationmentioning

confidence: 99%

Colistin- and Carbapenem-Resistant Escherichia coli Harboring mcr-1 and bla _NDM-5 , Causing a Complicated Urinary Tract Infection in a Patient from the United States

Mediavilla

Patrawalla

Chen

et al. 2016

mBio

199

109

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Colistin is increasingly used as an antibiotic of last resort for the treatment of carbapenem-resistant Gram-negative infections. The plasmid-borne colistin resistance gene mcr-1 was initially identified in animal and clinical samples from China and subsequently reported worldwide, including in the United States. Of particular concern is the spread of mcr-1 into carbapenem-resistant bacteria, thereby creating strains that approach pan-resistance. While several reports of mcr-1 have involved carbapenem-resistant strains, no such isolates have been described in the United States. Here, we report the isolation and identification of an Escherichia coli strain harboring both mcr-1 and carbapenemase gene blaNDM-5 from a urine sample in a patient without recent travel outside the United States. The isolate exhibited resistance to both colistin and carbapenems, but was susceptible to amikacin, aztreonam, gentamicin, nitrofurantoin, tigecycline, and trimethoprim-sulfamethoxazole. The mcr-1- and blaNDM-5-harboring plasmids were completely sequenced and shown to be highly similar to plasmids previously reported from China. The strain in this report was first isolated in August 2014, highlighting an earlier presence of mcr-1 within the United States than previously recognized.

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Antibiotic-resistant ST38, ST131 and ST405 strains are the leading uropathogenicEscherichia coliclones in Riyadh, Saudi Arabia

Abstract: Our findings highlight the presence of MDR, CTX-M-positive ST38, ST131 and ST405 UPEC in Saudi Arabia. The high proportion of isolates with CTX-M is a particular concern. We suggest that ST38 UPEC warrant further study.

Cited by 81 publications

References 21 publications

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Antibiotic resistance in Escherichia coli in husbandry animals: the African perspective

Colistin- and Carbapenem-Resistant Escherichia coli Harboring mcr-1 and bla _NDM-5 , Causing a Complicated Urinary Tract Infection in a Patient from the United States

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Antibiotic-resistant ST38, ST131 and ST405 strains are the leading uropathogenicEscherichia coliclones in Riyadh, Saudi Arabia

Abstract: Our findings highlight the presence of MDR, CTX-M-positive ST38, ST131 and ST405 UPEC in Saudi Arabia. The high proportion of isolates with CTX-M is a particular concern. We suggest that ST38 UPEC warrant further study.

Cited by 81 publications

References 21 publications

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Marine Bivalve Mollusks As Possible Indicators of Multidrug-Resistant Escherichia coli and Other Species of the Enterobacteriaceae Family

Antibiotic resistance in Escherichia coli in husbandry animals: the African perspective

Colistin- and Carbapenem-Resistant Escherichia coli Harboring mcr-1 and bla NDM-5 , Causing a Complicated Urinary Tract Infection in a Patient from the United States

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Product

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Colistin- and Carbapenem-Resistant Escherichia coli Harboring mcr-1 and bla _NDM-5 , Causing a Complicated Urinary Tract Infection in a Patient from the United States