Antibiotic sensitivity profile of multidrug-resistant (MDR) Escherichia coli isolated from dairy cow's milk in Probolinggo, Indonesia

Widodo, Agus; Lamid, Mirni; Effendi, Mustofa Helmi; Khairullah, Aswin Rafif; RIWU, KATTY HENDRIANA PRISCILIA; Yustinasari, Lita Rakhma; KURNIAWAN, SHENDY CANADYA; Ansori, Arif Nur Muhammad; Silaen, Otto Sahat Martua; Dameanti, Fidi Nur Aini Eka Puji

doi:10.13057/biodiv/d231002

Cited by 15 publications

(18 citation statements)

References 25 publications

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“…This research affirms that Multidrug resistance (MDR) and extended-spectrum betalactamase (ESBL) producing Escherichia coli can be found in several dairy farms in Probolinggo district, East Java, Indonesia. The results are similar to previous research, which identified MDR E. coli from dairy cow's milk (Widodo et al 2022), raw milk samples from the shelter (Anshariesta et al 2021) and ESBL-producing E. coli from fecal dairy cows sample in East Java, Indonesia (Putra et al 2020).…”

Section: Discussionsupporting

confidence: 91%

“…The use of broad-spectrum antibiotics, such as tetracycline and β-lactams is more common in cases of clinical mastitis in dairy cattle, because of their effective results. Tetracycline and aminoglycoside groups are the primary choice of antibiotics for respiratory and digestive system issues, followed by a combination of sulfonamide-trimethoprim medications that have a considerable impact on rumen microbial activity, and thirdgeneration cephalosporin antibiotics (Economou et al 2015;Widodo et al 2022). The percentage pattern of MDR E. coli isolates to several antibiotic agents can be caused by inappropriate antibiotic administration or excessive antibiotic administration in the treatment of infectious diseases in dairy cattle (Chowdhury et al 2021) and can also be caused by contamination from the environment and farmers during the milking process or livestock activities that allow transmission of E. coli with multidrug resistant characteristics can occur (Ahmedsham et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…In Indonesia, the first occurrence of ESBL E. coli was found in a milk sample from a dairy farm in West Java (Sudarwanto et al 2017). Reported on several samples from dairy farms in Yogyakarta province in 2021 (Maulana et al 2021), from dairy farm feces in East Java in 2019 (Putra et al 2019), from raw milk samples in 2021 (Ansharieta et al 2021), and milk samples from dairy cows in East Java in 2022 (Widodo et al 2022), its was found that E. coli from the samples contained ESBL encoding gene.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance characteristics of multidrug resistance and extended-spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia

et al. 2023

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Abstract. Widodo A, Lamid M, Effendi MH, Khailrullah AR, Kurniawan SC, Silaen OSM, Riwu KHP, Yustinasari LR, Afnani DA, Dameanti FNAEP, Ramandinianto SC. 2022. Antimicrobial resistance characteristics of multidrug resistance and extended spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia. Biodiversitas 23: 215-221. Escherichia coli bacteria initially reside in the digestive tract of humans and animals but are able to adapt to new environments that are different from their initial habitat. The pathogenicity of E. coli can occur when these bacteria grow more than normal limits, produce toxins, and are resistant to certain types of antibiotics. The purpose of this study was to investigate the antimicrobial resistance characteristics of MDR and ESBL-producing E. coli from several dairy farms in the Probolinggo district of East Java province, Indonesia. A total of 150 samples consisting of 109 milk and 41 environmental samples from 41 dairy farms were used for isolation. TSIA and IMViC biochemical tests were used to identify E. coli bacteria. Escherichia coli resistance profile was obtained through disc diffusion test on several antibiotics, namely tetracycline, streptomycin, trimethoprim, chloramphenicol and aztreonam. Escherichia coli that was resistant to 3 or more antibiotics was defined as MDR. The results of isolation and identification obtained 124 (82.6%) isolates characterizing E. coli bacteria. The antimicrobial susceptibility test of E. coli showed 9 (7.26%) MDR isolates and 2 (22.22%) ESBL isolates by double-disc synergy test (DDST). MDR E. coli was dominated by the pattern of antimicrobial drug resistance TE-S-W (tetracycline, streptomycin, trimethoprim) with a total of 8 (38.10%) isolates, followed by antimicrobial drug resistance pattern TE-S-W-ATM (tetracycline, streptomycin, trimethoprim, aztreonam) with one (4.76%) E. coli isolates. The pattern of antimicrobial drugs of ESBL E. coli showed in one (11.11%) sample of ESBL E. coli from a milk sample with the pattern of TE-S-W-ATM (tetracycline, streptomycin, trimethoprim, aztreonam) and one (11.11%) sample ESBL E. coli (AL 30) from the environmental sample with a pattern of TE-S-W pattern (tetracycline, streptomycin, trimethoprim). The discovery of MDR E. coli isolates and ESBL E. coli from milk and environmental samples at several dairy farms in Probolinggo district, East Java, Indonesia is a matter of concern and requires real action to reduce antibiotic resistance.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance characteristics of multidrug resistance and extended-spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia

et al. 2023

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“…Mastitis can be split into two categories based on bacterial contamination: mastitis brought on by agents from within the quarter and mastitis brought on by environmental contamination agents (Cobirka et al 2020). Compared to subclinical mastitis produced by environmental disease agents, the incidence rate of disease caused by pathogens from inside the quarter is higher (Widodo et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Detection of multidrug-resistant Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia

PUTRA

Khairullah²,

Effendi³

et al. 2023

Biodiversitas

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Abstract. Putra GDS, Khairullah AR, Effendi MH, Lazuardi M, Kurniawan SC, Afnani DA, Silaen OSM, Waruwu YKK, Millannia SK, Widodo A, Ramadhani S, Farizqi MTI, Riwu KHP. 2023. Detection of multidrug-resistant (MDR) Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia. Biodiversitas 24: 423-430. Bacterial resistance has emerged as a major concern in dairy farms in Indonesia due to the pervasive usage of antibiotics. Furthermore, no specific research has been done to explain the prevalence of Staphylococcus aureus in isolated milk from dairy cows in Kediri, particularly in Medowo Village, and their antibiotic resistance. Moreover, to control the emergence of diseases in humans brought on by dairy cow's milk or infections transmitted through milk, additional research on the prevalence and resistance of bacteria in dairy farms in the Kediri district is urgently required. In Medowo, the Village Kandangan of District, Kediri Regency, Indonesia, 100 dairy cow's milk samples were taken from farms in numerous hamlets. The Kirby-Bauer method performed an antibiotic sensitivity test using disk diffusion. The sensitivity test was attached with antibiotic discs on tetracycline, penicillin, gentamicin, erythromycin, and cefoxitin. According to sample evaluation results, S. aureus was detected in 94 (94%) of the 100 isolated milk samples based on morphological culture features, Gram staining, and biochemical assays. According to the profile of antibiotic resistance derived from the findings of the S. aureus antibiotic resistance test, 23 isolates (24.47%) were proven to be multidrug-resistant (MDR) because they were resistant to three to four classes of antibiotics. The use of antibiotics typically rises in response to an increase in the prevalence of disease in cattle, which could lead to higher levels of antibiotic residue in milk and possibly higher levels of bacterial resistance to antibiotics. Therefore, dairy farming requires methods for prudently and correctly employing antibiotics.

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“…Setting the distance between cows, placing pens far from the kitchen, isolating cows in separate pens, brooming manure, and regularly washing dairy cows prevent the spread of MDR bacteria in dairy farms (Pandit et al 2021). However, groups of dairy cows close to one another can result in MDR bacterial infections (Widodo et al 2022). Control of bacterial diseases requires surveillance and laboratory tests (Langley et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Detection of multidrug-resistant (MDR) Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in cow milk and hands of farmers in East Java, Indonesia

et al. 2023

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Abstract. Khairullah AR, Kurniawan SC, Sudjarwo SA, Effendi MH, Afnani DA, Silaen OSM, Putra GDS, Riwu KHP, Widodo A, Ramandinianto SC. 2023. Detection of multidrug-resistant Staphylococcus aureus and coagulase-negative staphylococci in cow milk and hands of farmers in East Java, Indonesia. Biodiversitas 24: 658-664. Cow milk is a nutrient-dense food containing various nutrients, i.e., carbohydrates, fat, and protein. However, it can potentially spread several pathogenic bacteria, such as staphylococci. Staphylococci cause public health problems. Staphylococcal strains were divided into two groups based on coagulase characteristics, namely coagulase-positive and coagulase-negative staphylococci (CoNS). Coagulase-positive was represented by Staphylococcus aureus. S. aureus and CoNS can become resistant to several antibiotics, known as multidrug resistance (MDR) bacteria. S. aureus and CoNS bacteria are known as causal agents in human infections. This study aims to identify the presence of multidrug resistance S. aureus and CoNS bacteria in several dairy farms in East Java, Indonesia. We collected 332 milk and 125 swab samples from the hand of farmers from several dairy farms in Probolinggo, Tulungagung, and Blitar of East Java, Indonesia. The collected S. aureus and CoNS were tested for antibiotic susceptibility using the disc diffusion method. The antibiotic used were cefoxitin, erythromycin, gentamicin, tetracycline, and oxacillin. The results of isolation and identification based on morphological and biochemical characteristics showed that out of 457 samples, 291 samples (63.68%) were positive for S. aureus, and 166 samples (36.32%) were positive for CoNs. There were 15 S. aureus isolates (5.15%) and 7 CoNS isolates (4.22%) confirmed as MDR; therefore, it is confirmed that S. aureus and CoNS resistance to various drugs was very high in the province of East Java. Intensive livestock systems, high livestock densities, and excessive use of antibiotics in modern dairy systems may predispose the developing growth of MDR S. aureus and CoNS infections. In conclusion, the results of this study indicate the presence of very high multidrug resistant S. aureus and CoNS in the East Java provinces. MDR bacteria can be prevented from spreading by improving biosecurity, hygiene in the milking process, and regular health care for animals and dairy workers.

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Antibiotic sensitivity profile of multidrug-resistant (MDR) Escherichia coli isolated from dairy cow's milk in Probolinggo, Indonesia

Cited by 15 publications

References 25 publications

Antimicrobial resistance characteristics of multidrug resistance and extended-spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia

Antimicrobial resistance characteristics of multidrug resistance and extended-spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia

Detection of multidrug-resistant Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia

Detection of multidrug-resistant (MDR) Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in cow milk and hands of farmers in East Java, Indonesia

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