Antibiotic susceptibility and mechanisms of β-lactam resistance among clinical strains of Pseudomonas aeruginosa: First report in Algeria

Drissi, Mourad; Ahmed, Z. Baba; Dehecq, Barbara; Bakour, Rabah; Plésiat, Patrick; Hocquet, Didier

doi:10.1016/j.medmal.2008.01.009

Cited by 18 publications

(18 citation statements)

References 13 publications

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“…The reduction in MICs of ticarcillin, aztreaonam, and meropenem in the presence of an efflux pump inhibitors (DNP or DCCD) suggested the contribution of an efflux-mediated mechanism in tested P. aeruginosa isolates to different antibiotics. This finding was consistent to other reports which showed a major contribution of efflux as the major resistance mechanism in P. aeruginosa (Drissi et al, 2008).…”

Section: Discussionsupporting

confidence: 93%

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in Pseudomonas aeruginosa

Zowalaty

Gyetvai

2016

Polish Journal of Microbiology

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A b s t r a c tPseudomonas aeruginosa is a leading human pathogen that causes serious infections at various tissues and organs leading to life threatening health problems and possible deadly outcomes. Resistance patterns vary widely whether it is from hospitals or community acquired infections. Reporting resistance profiles to a certain antibiotics provide valuable information in a given setting, but may be extrapolated outside the sampling location. In the present study, P. aeruginosa isolates were screened to determine their susceptibilities against antipseudomonal antimicrobial agents and possible existing mechanisms of resistance were determined. Eighty-six isolates of P. aeruginosa were recovered. Isolates representing different resistance profiles were screened for the existence of three different resistance mechanisms including drug inactivation due to metallo-β-lactamases, drug impermeability by outer membrane proteins and drug efflux. All tested isolates showed uniform susceptibility (100%, n = 86/86) to piperacillin, meropenem, amikacin, and polymyxin B. A single isolate was found to be imipenem resistant (99%, n = 85/86). The possible mechanisms of resistance of P. aeruginosa to imipenem involve active drug efflux pumps, outer membrane impermeability as well as drug inactivating enzymes. These findings demonstrate the fundamental importance of the in vitro susceptibility testing of antibiotics prior to antipseudomonal therapy and highlight the need for a continuous antimicrobial resistance surveillance programs to monitor the changing resistance patterns so that clinicians and health care officials are updated as to the most effective therapeutic agents to combat the serious outcomes of P. aeruginosa infections. This copy is for personal use only -distribution prohibited.El Zowalaty M.E. and Gyetvai B.1 24 anti biotics (El Zowalaty et al., 2015). The literature is rich in surveillance studies from all over the world reporting varying resistance rates among P. aeruginosa against different antimicrobial agent. Recently, studies have focused on the decreased susceptibility of P. aeru ginosa to currently used antipseudomonal agents, including β-lactams, aminoglycosides, and fluoroquinolones (Al-Tawfiq, 2007) since resistance of P. aeru ginosa to carbapenems, piperacillin, and other highly active antibiotics has emerged and is increasing which makes treatment of P. aeruginosa infections troublesome (Strateva et al., 2007).Recently, resistance rates of P. aeruginosa clinical isolates recovered from patients admitted to Zagazig University hospitals in Egypt against different classes of antimicrobial agents were reported (El Zowalaty, 2012). The current study further examined the susceptibilities and possible resistance mechanisms of P. aeruginosa isolates collected from hospitalized patients against selected antipseudomonal agents that are available in the Egyptian pharmaceutical market and are frequently prescribed to patients. Experimental Materials and MethodsStudy site. The specimens were collected from ...

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Section: Discussionsupporting

confidence: 93%

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in Pseudomonas aeruginosa

Zowalaty

Gyetvai

2016

Polish Journal of Microbiology

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“…The prevalence of AmpC β-lactamase among the isolates in the present study is higher than in previously reported data from Algeria where a 12% overproduction of AmpC was documented [31]. Our findings were, however, lower in comparison to 59.4% from India [32] and 38.0% reported from Ghana [33].…”

Section: Discussioncontrasting

confidence: 90%

First Detection of OXA-10 Extended-Spectrum Beta-Lactamases and the Occurrence of mexR and nfxB in Clinical Isolates of Pseudomonas aeruginosa from Nigeria

Odumosu¹,

Adeniyi

Chandra

2015

Chemotherapy

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Background: The characterization of β-lactamase production in Pseudomonasaeruginosa is rarely reported in Nigeria. The objective of this study was to investigate the occurrence and characterize the different β-lactamases as well as mechanisms of fluoroquinolones resistance among P. aeruginosa isolated from various clinical sources from Nigeria. Materials and Method: Isolates were investigated using PCR, RFLP and sequencing for the detection of various β-lactamases and efflux pump regulator genes. Result: The prevalence of OXA-10, AmpC, CTX-M and SHV in P. aeruginosa was 80, 70, 5 and 5%, respectively. The coexistence of bla_OXA-10 with bla_AmpC, bla_SHV and bla_CTX-M was reported in 40, 5 and 5% of isolates, respectively. The efflux pump regulator genes mexR and nfxB were both amplified in 45% of the OXA-10-positive isolates. Conclusion: This is the first report of the characterization of OXA-10 extended-spectrum β-lactamases and occurrence of mexR and nfxB efflux regulator genes in clinical isolates of P. aeruginosa in Nigeria.

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“…aeruginosa infections are commonly treated with a combination of ␤-lactams and aminoglycosides. Resistance to the ␤-lactam antibiotics is frequently observed in the clinical setting and is commonly due to the expression of enzymes that can hydrolyze these compounds, namely, ␤-lactamases (13,(49)(50)(51)(52). P. aeruginosa clinical isolates often harbor acquired ␤-lactamases, particularly those of the oxacillinase type (class D) and the metallo-␤-lactamase IMP and VIM types (class B) (23,25,(53)(54)(55)(56)(57)(58).…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Carbapenem-Hydrolyzing Enzyme, PoxB, in Pseudomonas aeruginosa PAO1

Zincke

Balasubramanian

Silver³

et al. 2016

Antimicrob Agents Chemother

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cPseudomonas aeruginosa is an opportunistic pathogen often associated with severe and life-threatening infections that are highly impervious to treatment. This microbe readily exhibits intrinsic and acquired resistance to varied antimicrobial drugs. Resistance to penicillin-like compounds is commonplace and provided by the chromosomal AmpC ␤-lactamase. A second, chromosomally encoded ␤-lactamase, PoxB, has previously been reported in P. aeruginosa. In the present work, the contribution of this class D enzyme was investigated using a series of clean in-frame ampC, poxB, and oprD deletions, as well as complementation by expression under the control of an inducible promoter. While poxB deletions failed to alter ␤-lactam sensitivities, expression of poxB in ampC-deficient backgrounds decreased susceptibility to both meropenem and doripenem but had no effect on imipenem, penicillin, and cephalosporin MICs. However, when expressed in an ampCpoxB-deficient background, that additionally lacked the outer membrane porin-encoding gene oprD, PoxB significantly increased the imipenem as well as the meropenem and doripenem MICs. Like other class D carbapenem-hydrolyzing ␤-lactamases, PoxB was only poorly inhibited by class A enzyme inhibitors, but a novel non-␤-lactam compound, avibactam, was a slightly better inhibitor of PoxB activity. In vitro susceptibility testing with a clinical concentration of avibactam, however, failed to reduce PoxB activity against the carbapenems. In addition, poxB was found to be cotranscribed with an upstream open reading frame, poxA, which itself was shown to encode a 32-kDa protein of yet unknown function.

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Antibiotic susceptibility and mechanisms of β-lactam resistance among clinical strains of Pseudomonas aeruginosa: First report in Algeria

Cited by 18 publications

References 13 publications

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in Pseudomonas aeruginosa

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in Pseudomonas aeruginosa

First Detection of OXA-10 Extended-Spectrum Beta-Lactamases and the Occurrence of <b><i>mexR </i></b>and <b><i>nfxB</i></b> in Clinical Isolates of <b><i>Pseudomonas aeruginosa</i></b> from Nigeria

Characterization of a Carbapenem-Hydrolyzing Enzyme, PoxB, in Pseudomonas aeruginosa PAO1

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