Antibiotic susceptibility of Salmonella, Shigella and Vibrio isolated from diarrhea patients in Jakarta, Indonesia

Meiyanti, Meiyanti; Salim, Oktavianus Ch.; Herwana, Elly; Kalumpiu, Joice Viladelvia; Lesmana, Murad

doi:10.20885/jkki.vol7.iss3.art4

Cited by 4 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…is the most common bacterium that causes bacillary dysentery (shigellosis), a disease characterized by damage to the colonic mucosa caused by bacterial invasion (Garcia et al, 2017). This member of Shigella has also been reported to be resistant to several antibiotics, such as ampicillin, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole (Meiyanti et al, 2016). Again, a thorough investigation is required to prove it.…”

Section: Identification Of Bacteria Based On the 16s Rrna Genementioning

confidence: 99%

Genetic Identification of Superbugs from River Streams in State University of Malang

Sholekah

Listyorini

2023

JRBA

View full text Add to dashboard Cite

Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.

show abstract

Section: Identification Of Bacteria Based On the 16s Rrna Genementioning

confidence: 99%

Genetic Identification of Superbugs from River Streams in State University of Malang

Sholekah

Listyorini

2023

JRBA

View full text Add to dashboard Cite

show abstract

“…S sonnei dan S boidy hampr 75%-100% resisten terhadap tetrasiklin dan trimethoprimsulfamethosasol. Sedangkan semua isolat S dysenteriae (100%) resisten terhadap klorampenikol dan trimetoprimsulfamethoxasol (11).…”

Section: Pendahuluanunclassified

Indeks adhesi Shigella dysenteriae pada enterosit mencit galur BALB/ pasca pemaparan protein pili 42 kDa

Suswati¹,

Purnamasari²,

Wisudanti³

et al. 2020

JKS

View full text Add to dashboard Cite

Abstrak. Tujuan penelitian ini adalah membuktikan bahwa protein pili Shigella dysenteriae dengan berat molekul 42 kDa merupakan protein adhesi dari S. dysenteriae pada enterosit mencit galur BALB/c. Penelitian ini merupakan penelitian eksperimental laboratoris yang dilaksanakan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Jember, melalui beberapa tahap yaitu isolasi dan identifikasi S. dysenteriae, kultur S. dysenteriae, isolasi pili, SDS-PAGE, pemurnian protein pili, uji hemaglutinasi,isolasi enterosit mencit galur BALB/c, dan uji adhesi. Pada penelitian ini dibentuk 6 kelompok perlakuan dan 1 kontrol negatif. Keenam kelompok perlakuan tersebut meliputi konsentrasi protein pili 1, ½, ¼, 1/8, 1/16, dan 1/32. Data dianalisis menggunakan program statistik SPSS (Statistical Product and Service Solution) versi 16, jenis regresi linier sederhana dan one way Anov. Hasil penelitian menunjukkan bahwa protein pili S. dysenteriae 42 kDa mampu menghambat perlekatan S. dysenteriae terhadap enterosit mencit galur BALB/c. Pada uji regresi linier sederhana diperoleh nilai R square 0,897 yang berarti nilai indeks adhesi dipengaruhi oleh konsentrasi protein pili sebesar 89,7%, dan 10,3% dipengaruhi oleh faktor lain. Hasil uji one way Anova menunjukkan bahwa perbedaan konsentrasi protein pili berpengaruh terhadap indeks adhesi bakteri dengan nilai Sig. = 0,000 (p value 0,05). Kesimpulan yang diperoleh dari penelitian ini sesuai dengan hipotesis, yaitu protein pili dengan berat molekul 42 kDa merupakan protein adhesi dari Shigella dysenteriae pada enterosit mencit galur BALB/c. Kata kunci: Shigella dysenteriae, protein pili 42 kDa, indeks adhesi Abstract. The aim of this study was to prove that the protein pili Shigella dysenteriae with a molecular weight of 42 kDa is an adhesion protein from S. dysenteriae in enterocytes of BALB / c mice. This research is a laboratory experimental research carried out at the Microbiology Laboratory of the Faculty of Medicine, University of Jember, through several stages, namely the isolation and identification of S. dysenteriae, culture of S. dysenteriae, isolation of pili, SDS-PAGE, purification of pili protein, hemagglutination test, isolation of enterocyte strain mice. BALB / c, and adhesion test. In this study, 6 treatment groups and 1 negative control were formed. The six treatment groups included protein concentrations of pili 1, ½, ¼, 1/8, 1/16, and 1/32. The data obtained were analyzed using the statistical program SPSS (Statistical Product and Service Solution) version 16, simple linear regression and one way Anov. The results showed that the 42 kDa S. dysenteriae pili protein was able to inhibit the attachment of S. dysenteriae to enterocytes of the BALB / c mice. In the simple linear regression test, it was obtained that the R square value was 0.897, which means that the adhesion index value was influenced by the pili protein concentration of 89.7%, and 10.3% was influenced by other factors. The one way Anova test results showed that the difference in pili protein concentration had an effect on the bacterial adhesion index with the Sig. = 0.000 (p value 0.05). The conclusion obtained from this study is in accordance with the hypothesis, namely pili protein with a molecular weight of 42 kDa is the adhesion protein of Shigella dysenteriae in enterocytes of BALB / c mice. Key words: Shigella dysenteriae, 42 kDa pili protein, adhesion index

show abstract

“…In many regions of the world, about half of Shigella strains are currently drug-resistant with a variety of mechanisms for antibiotic resistance (Ranjbar and Farahani 2019). Different bacterial isolates had varying levels of resistance as S. flexneri exhibited the highest level of resistance to ampicillin, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole (80%-90%); most strains of S. sonnei and S. boydii exhibited tetracycline and trimethoprim-sulfamethoxazole resistance (75%-100%), and all S. dysenteriae exhibited chloramphenicol and trimethoprim-sulfamethoxazole resistance (100%) (Meiyanti et al 2016). Others found that more than 73% of S. flexneri isolates were resistant to all four tested antibiotics: ampicillin, chloramphenicol, tetracycline, and trimethoprim-sulfamethoxazole (Agtini et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Abundance and antimicrobial susceptibility of Shigella spp. isolated from aquatic environments in Banda Aceh, Indonesia

2023

View full text Add to dashboard Cite

Abstract. Zahara Y, Suhartono S, Nirliana N. 2022. Abundance and antimicrobial susceptibility of Shigella spp. isolated from aquatic environments in Banda Aceh, Indonesia. Biodiversitas 23: 6420-6427. Accumulating pathogenic bacteria in aquatic systems increases waterborne infections, including shigellosis. The infection is causatively associated with Shigella spp. within the water system. This study aimed to isolate and determine the abundance of Shigella spp. collected from three rivers in Banda Aceh, Indonesia, namely Krueng Lamnyong, Krueng Aceh, and Krueng Neng water, and their antibiotic susceptibility. The bacterial enumeration was conducted using the MPN method followed by bacterial isolation using XLD and SSA selective media, whereas antibiotic susceptibility was evaluated using the standards of CLSI. Results showed that there was no significant difference (p=0.059) in the bacterial abundance among rivers i.e., 4.67 MPN/mL in Krueng Lamnyong, 27.33 MPN/mL in Krueng Aceh, and 18.33 MPN/mL in Krueng Neng. In total, 19 isolates of Shigella spp. were isolated, of which four isolates were from Krueng Lamnyong, nine isolates from Krueng Aceh, and six isolates from Krueng Neng. Antibiotic susceptibility test showed that the isolates had resistance to tetracycline, penicillin, and nalidixic acid. More than half of the isolates were resistant to more than five antibiotic classes. The study indicates that rivers are the main reservoir for the transmission of pathogenic bacteria in aquatic systems and some efforts are needed to reduce potential health risks and ensure water quality in aquatic systems.

show abstract

Antibiotic susceptibility of Salmonella, Shigella and Vibrio isolated from diarrhea patients in Jakarta, Indonesia

Cited by 4 publications

References 17 publications

Genetic Identification of Superbugs from River Streams in State University of Malang

Genetic Identification of Superbugs from River Streams in State University of Malang

Indeks adhesi Shigella dysenteriae pada enterosit mencit galur BALB/ pasca pemaparan protein pili 42 kDa

Abundance and antimicrobial susceptibility of Shigella spp. isolated from aquatic environments in Banda Aceh, Indonesia

Contact Info

Product

Resources

About