Antibiotic Susceptibility Pattern and Identification of Multidrug Resistant Novel <i>Salmonella</i> Strain in Poultry Chickens of Hathazari Region in Chattogram, Bangladesh

Abstract

Poultry chickens are potential source of transmission of zoonotic Salmonella, into human food chain, causing food-borne illness and also hindering development of poultry industry in Bangladesh. The non-judicious uses of antibiotics in poultry farm have increased the multidrug resistant bacteria. So, this study reports the occurrence of Salmonella in poultry samples (meat, egg, liver and cloacal swab) and the antimicrobial resistance pattern of the isolates. This study was carried out throughout the period of May 2019-March 2020, at the bacteriological laboratory in the Department of Microbiology, University of Chittagong. Isolates were identified on the basis of cultural and biochemical tests from a total of 25 broiler samples (meat, liver, eggshell and cloacal swab). Antibiotic susceptibility pattern was observed using Kirby-Bauer disk diffusion method. The overall detection rate of Salmonella was 48% (12/25) and the highest occurrence was noticed in raw meat 62.5% and the lowest in liver (37.5%). The antimicrobial resistance tests revealed that all the isolates (n = 12) exhibited 100% resistance to vancomycin and cephalexin, followed by ampicillin (75%), nalidixic acid (58.33%), chloramphenicol (41.66%), doxycycline (50%), and neomycin (50%). On the other hand, ciprofloxacin showed 83.33%, ceftazidime and amoxicillin showed 91.6% sensitivity respectively. A considerably high proportion of isolates (11/12, 91.67%) was resistant to three or more antibiotics and 6 multidrug profiles were observed. The ampicillin-chloramphenicol-nalidixic acid-neomycin-cephalexindoxycycline-vancomycin (4/12) was more frequently observed phenotype in multidrug profiles. Finally, two multidrug-resistant strains of Salmonella

Synergistic influence of probiotic and florfenicol on embryonic viability, performance, and multidrug-resistant Salmonella Enteritidis in broiler chickens

“…Meanwhile, low resistance was recorded with DO, SXT, FFC (46.15%, for each) and N (30.77%). These findings were in near similarity with those of Hardiati et al 38 , who reported that Salmonella isolated from chicken farms was resistant to OT and ampicillin (AM) with (75%), Islam et al 39 , who showed resistance against AM (75%), N (50%) and DO (50%). Meanwhile, Velasquez et al 36 recorded a higher resistance of Salmonella isolates against S, and Alam et al 40 recorded resistance against AM and S of 82.85% and 77.14%, respectively.…”

An Alternative Approach Using Nano-garlic Emulsion and its Synergy with Antibiotics for Controlling Biofilm-Producing Multidrug-Resistant Salmonella in Chicken

“…Antibiotic resistance can develop as a result of excessive antibiotic usage [ 3 , 42 , 43 ]. In this study, the antimicrobial resistance profile among Salmonella isolates was nearly similar to those recorded in Egypt [ 6 ], China [ 44 ], and Bangladesh [ 31 ].…”

“…In the current study, the prevalence rate was 10% which is consistent with previous studies by Ammar et al [ 28 ] and Ibrahim et al [ 29 ] in Egypt. However, a relatively higher isolation rate of 35.9% was observed in Ethiopia by Berihun et al [ 30 ] and 37.5% in Bangladesh [ 31 ]. On the other hand, Helmy et al [ 32 ] recorded a lower percentage of 3.4% from apparently healthy chickens in Egypt.…”