Antibiotics are used in the prevention of different kinds of infectious diseases, but now-a-days, multidrug resistant strain of pathogenic bacteria is increasing due to the excess or misuse of antibiotics. These antibiotic resistant bacteria have become a great problem for the big population of Bangladesh. In this study, seven clinical samples were screened from about 250 patients having upper respiratory infections at Gonoshasthya Nagar Hospital, Dhanmondi, Dhaka. The isolated S. aureus was screened for their antibiotic resistance profiles. In this study, eleven antimicrobial drugs were used. The objective of this study was to check the sensitivity and resistance ability of S. aureus in different antibiotic concentrations and growth rate at room temperature after isolation, purification and characterization because infectious diseases could play a great role in human health in developing countries due to multidrug resistance activity of bacteria. S. aureus was found to be resistant to eight commonly used antibiotics and concentration of the organism was reasonably high in the urine sample followed by others. In room temperature, it grows continuously for about 10 h without changing the growth media and then the growth rate is decreased gradually with a small stationary phase. In future study, it could be helpful to find out more information on the relationship between this multidrug resistance microorganism and antibiotics with the solution of S. aureus related diseases.