Antibiotic Susceptibility Profile, ESBL Production and blaCTX-M1, blaSHV and blaTEM Types Among Escherichia coli Blood Isolates

Nojoomi, Farshad; Ghasemian, Abdolmajid; Eslami, Majid; Khodaparast, Sepideh

doi:10.15171/ijep.2017.03

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…According to EUCAST (European Committee on Antimicrobial Susceptibility Testing) breakpoints (http://www.eucast.org/clinical_breakpoints/), isolates with (MIC) 2 μg/ml were considered to be colistin-resistant. Isolates were also subjected to polymerase chain reaction (PCR) using published primers [22] to ascertain whether particular strains contained the (mcr-types) genes. Isolates that have been found to be resistant to three or more antimicrobials from different classes are known as multi-drug resistant (MDR) isolates [14].…”

Section: Antibiotics-susceptibility Patternsmentioning

confidence: 99%

Molecular Detection of Plasmid-mediated colistin-resistance genes in Multi-drug resistant Escherichia coli isolated from human and poultry in Duhok city, Iraq

said,

yassin

2024

Egyptian Journal of Veterinary Sciences

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HE plasmid-borne colistin resistance gene mcr-types were formerly recognized as plasmidmediated colistin mechanism among clinical and animal samples Escherichia coli from China and thereafter reported worldwide. The study investigated for screening of plasmid-mediated colistin and carbapenems resistant genes in Escherichia coli isolates from human and poultry origin. After isolation and identification of isolates from clinical specimens and poultry cloacal swab, disc diffusion and broth microdilution method were determined antibiotic susceptibility patterns, phenotypic Extended-Spectrum Beta-lactamase enzymes (ESBL)-production test and phenotypic colistin resistance. Molecular detection by PCR was applied to targeted carbapenems-resistance genes (KPC-2, OXA-48 and NDM-1) and colistin-resistant genes (mcr-1, mcr-2 and mcr-3). The majority of isolates were higher resistance to commonly used antibiotic categories with Multi-Drug Resistance (63 %) and (93 %) of clinical and poultry E coli isolate, respectively. Using broth microdilution (16%) of isolates displayed phenotypic colistin resistance. Human isolates had higher NDM-1 gene (33.3%), while, higher OXA-48 gene (22.2%) was in poultry isolates, and no KPC-2 gene was identified. Only isolates from poultry contained the mcr-1 gene (27.7%), while mcr-2 gene was present only in human isolates (4.7%) and mcr-3 gene in both human (33.3%) and poultry (22.2%) isolates. Negative mcr-1, 2, and 3 isolates expressed high rates of ESBLproduction. The coexistence of (mcr-3 + NDM-1) was frequent, including 10 isolates, and one isolate carried (mcr-2+mcr-3+NDM-1) combination from urine samples. Data of colistin resistance among clinical and poultry isolates in this region is scarce. Identification of colistin and carbapenems-resistance genes via plasmids among those isolates menace and approaches pan-resistance and failure treatment.

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Section: Antibiotics-susceptibility Patternsmentioning

confidence: 99%

Molecular Detection of Plasmid-mediated colistin-resistance genes in Multi-drug resistant Escherichia coli isolated from human and poultry in Duhok city, Iraq

said,

yassin

2024

Egyptian Journal of Veterinary Sciences

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“…Difficulties in eradication of drug-resistant infections have been a critical issue in recent years. [2][3][4][5][6] Great therapeutic properties of aquatic sources including sterols, peptides, terpenes and nitrogen compounds, macrolides, acid-fat derivatives, prostaglandins, and other alkaloids have been revealed. 7 Bacterial infections cause many deaths annually.…”

Section: Enteric Pathogensmentioning

confidence: 99%

Antimicrobial Features of Cerastoderma and Didacna Double Basins Peptides

Ghasemian¹,

Nojoomi²,

Najafiolya³

et al. 2018

Int J Enteric Pathog

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Background: In recent years, high attention has been given to the biological activities of natural compounds and their potential antimicrobial properties. Objective: In this study, the antibacterial properties of the extracts from tissue and peptides of Cerastoderma and Didacna were studied. Materials and Methods: samples of Cerastoderma and Didacna were collected and washed. Then, the soft tissues were cut and powdered, and concentrations of 16, 8, 4, 2, 1 and 0.5 of chloroform, ethanol and methanol, and in addition extract of enzymatic hydrolysis were prepared, and their antibacterial activities against Staphylococcus aureus, Escherichia coli and Salmonella paratyphi were investigated. The disc diffusion method was used for the evaluation of strains susceptibility. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were investigated for bacterial growth inhibition. Results: Methanolic and ethanolic extracts from Cerastoderma demonstrated higher growth inhibitory effects compared to those from Didacna on E. coli and S. paratyphi and exhibited similar activities against S. aureus at concentrations 16 and 8 ug/mL. In addition, chloroform extracts of Cerastoderma and Didacna displayed similar inhibitory effects on S. paratyphi and S. aureus at concentrations 16 and 8 ug/mL which was a suitable effect, and the extract from Cerastoderma was more effective. MIC and MBC of methanolic extracts were at the lowest level, especially against S. aureus. Conclusion: It was revealed that Cerastoderma and Didacna extracts were effective as antibacterial compounds on S. aureus, E. coli and S. paratyphi species as natural agents.

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The evolutionary puzzle of Escherichia coli ST131

Pitout

Finn²

2020

Infection, Genetics and Evolution

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Antibiotic Susceptibility Profile, ESBL Production and blaCTX-M1, blaSHV and blaTEM Types Among Escherichia coli Blood Isolates

Cited by 3 publications

References 7 publications

Molecular Detection of Plasmid-mediated colistin-resistance genes in Multi-drug resistant Escherichia coli isolated from human and poultry in Duhok city, Iraq

Molecular Detection of Plasmid-mediated colistin-resistance genes in Multi-drug resistant Escherichia coli isolated from human and poultry in Duhok city, Iraq

Antimicrobial Features of Cerastoderma and Didacna Double Basins Peptides

The evolutionary puzzle of Escherichia coli ST131

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