Antibiotic Susceptibility Profiles of Listeria monocytogenes Strains Isolated from Food Products and Clinical Samples

Caplan, M. E.; Mateescu, L. A.; Dimov, Tatiana Vassu; Rafilă, Alexandru; Borcan, Alina Maria

doi:10.2478/rrlm-2014-0014

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…This result is compatible with a study that was conducted in Romania in which all isolates of L. monocytogenes from meat, dairy products and clinical samples were very susceptible for imipenem, amikacin, gentamicin and chloramphenicol. 18 In the present study as in many other recent studies, Listeria monocytogenes is becoming increasingly resistant to a wide number of antimicrobials including the beta-lactam antibiotics, 18 which indicates that its danger on the human health is also becoming more and more preoccupying.…”

Section: Discussionsupporting

confidence: 60%

“…17 For this reason, they are frequently used in therapy for the infection with L. monocytogenes whereas the standard therapy is formed from amoxicillin or ampicillin in combination with an aminoglycoside like streptomycin. 18 However, isolated strains were very highly susceptible to imipenem (100%), amikacin (100%), gentamicin (90%), kanamycin (90%), sulfamethoxazole (80%) and chloramphenicol (70%). This result is compatible with a study that was conducted in Romania in which all isolates of L. monocytogenes from meat, dairy products and clinical samples were very susceptible for imipenem, amikacin, gentamicin and chloramphenicol.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence, characterization and antimicrobial resistance of Listeria monocytogenes isolated from beef meat in Meknes city, Morocco

Boukili¹,

Filali

Lafkih³

et al. 2020

Germs

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Introduction Listeria monocytogenes is one of the most pathogenic bacteria related to the consumption of contaminated food. This study aims to determine the prevalence of L. monocytogenes in raw beef meat in Meknes city of Morocco, to evaluate its pathogenicity and resistance to antimicrobials.Methods During four seasons, a total of 140 samples were collected from supermarkets, butcheries and Souk (weekly traditional market). The PCR method was used to examine the presence of specific and virulence genes in the isolated strains, and also to identify their serotypes. The antimicrobial resistance was determined.Results The results show a prevalence of 7.14% which depends on retail sites and also on the season's variation. The majority of the strains were detected in butcheries (6 strains), and supermarkets (4 strains). Moreover, the majority of strains were detected during summer (50%). Concerning virulence genes, the seven researched genes were detected in 100% of isolated strains. The majority of strains were of the (1/2a, 1/2c, 3a and 3c) serogroup (70%), while two of them were of the (1/2b, 3b, 4b and 4d) serogroup (20%). All isolates were resistant to at least one antimicrobial, while three strains were resistant to nine tested antimicrobials. However, they were highly susceptible to amikacin, imipenem, gentamicin, sulfamethoxazole and chloramphenicol.Conclusions According to results, isolated L. monocytogenes from analyzed beef meat shows a high level of pathogenicity and resistance to the most used antimicrobials in listeriosis therapy, which calls for the severe application of quality systems at the slaughterhouses and retail sites level.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Prevalence, characterization and antimicrobial resistance of Listeria monocytogenes isolated from beef meat in Meknes city, Morocco

Boukili¹,

Filali

Lafkih³

et al. 2020

Germs

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“…The variation in the results is related to differences in the rate of meat carcass contamination, isolation, and identification methodologies. In the hereby experiment, the isolates were identified to genus level using PCR considered the "gold standard" for identification of Listeria which is difficult to be accurately identified by only phenotypic methods used in some of the previous studies [35]. Therefore, those studies, including the Nigerian reports, in which traditional phenotypic methods were solely used for the identification of Listeria, might have deemphasized or overestimated the occurrence of Listeria in the meat samples [18].…”

Section: Discussionmentioning

confidence: 99%

Occurrence and antibiogram of Listeria species in raw pork, beef, and chicken meats marketed in Enugu State, Southeast Nigeria

et al. 2020

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Aim: This study was undertaken to isolate Listeria (L.) species from raw meats sold in markets in Enugu State, Southeast Nigeria, and to determine the antibacterial resistance profile. Materials and Methods: Twenty-five grams of beef (n=144), chicken meat (n=144), and pork (n=144) were collected randomly from supermarkets and general markets in Enugu State. Isolation of Listeria was done using half and full Fraser broths, and polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol agar. Identification of isolates was done using an analytical profile index kit specific for Listeria. Confirmation of the genus Listeria was done by a polymerase chain reaction. The resistance of the isolates was determined using the disk diffusion method. Results: Listeria was isolated from 39/144 (27.1%) chicken meat, 19/144 (13.2%) pork, and 66/144 (45.8%) beef samples cultured. Listeria innocua was the predominant species in chicken meat (52.6%) and beef (81.8%) samples. Listeria grayi, Listeria welshimeri, and Listeria ivanovii were also isolated from the beef and chicken meat samples. More than 65% of the isolates were resistant to penicillin, rifampicin, ciprofloxacin, sulfamethoxazole-trimethoprim, and cephalothin. All the isolates from beef and pork samples and 23 (92%) from chicken meat samples, were resistant to ≥3 classes of antibacterial agents. Mean multiple antibiotic resistance index (MARI) was 0.77 (range=0.42-1.00), 0.58 (range=0.25-0.83), and 0.79 (range=0.58-0.92) for the isolates from beef, chicken meat, and pork samples, respectively. All the isolates had MARI >0.2. Conclusion: Multidrug-resistant Listeria strains contaminate raw beef, pork, and chicken meats marketed in Enugu State, Southeast Nigeria.

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“…Optimal temperature range, a heat resistance temperature range of 20°C–60°C, oxidase, and catalase were determined; 1% (v/v) tetramethyl-p-phenylenediamine dihydrochloride and 3% (v/v) hydrogen peroxide, as well as NaCl tolerance salt concentration from 4 to 16% were all maintained ( Ray et al, 2012 ). Antibiotic disks containing vancomycin (30 μg), tetracycline (30 μg), cefotaxime (30 μg), streptomycin (10 μg), ciprofloxacin (10 μg), gentamicin (20 μg), cefuroxime (30 μg), cephalosporin (30 μg), penicillin G (10 μg), erythromycin (15 μg), and ampicillin sulbactam (30 μg) were used to test the antibiotic sensitivity of the NR1 strain culture ( Caplan et al, 2014 ). The nitrogen source utilization included L-histidine, aspartic acid, L-serine, L-alanine, L-ornithine, L-hydroxyproline, D-arginine, and glycine at a final concentration of 1% (w/v).…”

Section: Methodsmentioning

confidence: 99%

Whole-Genome and Transcriptome Sequencing-Based Characterization of Bacillus Cereus NR1 From Subtropical Marine Mangrove and Its Potential Role in Sulfur Metabolism

Kashif

Sang

et al. 2022

Front. Microbiol.

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Sulfur, organosulfur compounds, and sulfides are essential parts of life. Microbial sulfate assimilation is among the most active and ancient metabolic activities in the sulfur cycle that operates in various ecosystems. We analyzed the molecular basis of bacterial characterization. NR1 was isolated and purified from mangrove sediments. Whole-genome sequencing indicated that the NR1 isolate was closely related to Bacillus cereus. The genome contained 5,305 functional genes with a total length of 5,420,664 bp, a GC content of 35.62%, 42 rRNA, and 107 tRNA. DBT-grown cultures exhibited DBT utilization, fleeting emergence of DBT sulfone (DBTO2), and formation of 2-hydroxybiphenyl (2-HBP). Molecular analysis of the PCR products’ dsz operon revealed the presence of dszA, dszB, and dszC genes, which encoded for NR1’s 90% DBT desulfurization activity. Furthermore, 17 sulfur metabolism-related genes, including genes involved in assimilation sulfate reduction, APS and PAPS, and the cys, ssu, and TST gene families, were identified. In sulfate media, alkenesulfonate was converted to sulfite and inhibited ssu enzymes. Downregulated cysK variants were associated with nrnA expression and the regulation of L-cysteine synthesis. These findings established a scientific foundation for further research and application of bacteria to mangrove rehabilitation and ecological treatment by evaluating the bacterial characterization and sulfur degradation metabolic pathway. We used whole-genome and transcriptome sequencing to examine their genetic characteristics.

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Antibiotic Susceptibility Profiles of Listeria monocytogenes Strains Isolated from Food Products and Clinical Samples

Abstract: Listeria monocytogenes has a ubiquitous distribution in nature and

Cited by 6 publications

References 16 publications

Prevalence, characterization and antimicrobial resistance of Listeria monocytogenes isolated from beef meat in Meknes city, Morocco

Prevalence, characterization and antimicrobial resistance of Listeria monocytogenes isolated from beef meat in Meknes city, Morocco

Occurrence and antibiogram of Listeria species in raw pork, beef, and chicken meats marketed in Enugu State, Southeast Nigeria

Whole-Genome and Transcriptome Sequencing-Based Characterization of Bacillus Cereus NR1 From Subtropical Marine Mangrove and Its Potential Role in Sulfur Metabolism

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