Antibiotics against Amebiasis in Macaques 1,2

Anderson, Hamilton H.; Anderson, Jeanette Van D.

doi:10.4269/ajtmh.1950.s1-30.193

Cited by 10 publications

(4 citation statements)

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“…23,24 Importantly, these parameters are also known for these tetracyclines in macaques, the best animal models found to date for studying the development of AMD. [25][26][27][28][29] FLIM has now been adapted to an ophthalmoscope for use in humans; 19,20 therefore, we suggest that tetracycline-based FLIM could become a potentially valuable approach for detection of spherules as an early marker for the development of sub-RPE deposits. The recent discovery 6 that larger HAP deposits in the eye (tens of microns, termed nodules) confer high risk for progression to advanced AMD within one year further supports our contention that HAP spherules may be a useful biomarker for early detection of AMD.…”

Section: Discussionmentioning

confidence: 99%

Imaging hydroxyapatite in sub-retinal pigment epithelial deposits by fluorescence lifetime imaging microscopy with tetracycline staining

et al. 2020

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Significance: Recent evidence suggests that hydroxyapatite (HAP) in sub-retinal pigment epithelial (sub-RPE) deposits in aged human eyes may act to nucleate and contribute to their growth to clinically detectable size. Sub-RPE deposits such as drusen are clinical hallmarks of agerelated macular degeneration (AMD), therefore enhanced and earlier detection is a clinical need. We found that tetracycline-family antibiotics, long known to stain HAP in teeth and bones, can also label the HAP in sub-RPE deposits. However, HAP-bound tetracycline fluorescence excitation and emission spectra overlap with the well-known autofluorescence of outer retinal tissues, making them difficult to resolve.Aim: In this initial study, we sought to determine if the HAP-bound tetracyclines also exhibit enhanced fluorescence lifetimes, providing a useful difference in lifetime compared with the short lifetimes observed in vivo in the human retina by the pioneering work of Schweitzer, Zinkernagel, Hammer, and their colleagues, and thus a large enough effect size to resolve the HAP from background by fluorescence lifetime imaging.Approach: We stained authentic HAP with tetracyclines and measured the lifetime(s) by phase fluorometry, and stained aged, fixed human cadaver retinas with drusen with selected tetracyclines and imaged them by fluorescence lifetime imaging microscopy (FLIM).Results: We found that chlortetracycline and doxycycline exhibited substantial increase in fluorescence lifetime compared to the free antibiotics and the retinal background, and the drusen were easily resolvable from the retinal background in these specimens by FLIM.Conclusions: These findings suggest that FLIM imaging of tetracycline (and potentially other molecules) binding to HAP could become a diagnostic tool for the development and progression of AMD.

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Section: Discussionmentioning

confidence: 99%

Imaging hydroxyapatite in sub-retinal pigment epithelial deposits by fluorescence lifetime imaging microscopy with tetracycline staining

et al. 2020

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“…Hrenoff and Anderson (1950) and Anderson and Anderson (1950) found mixtures of polymyxin, streptomycin, and bacitracin to be effective in monkeys. In vivo.…”

Section: Polymyxins (Aerosporins)mentioning

confidence: 99%

“…Lynch and Stephens (1956) tested derivatives of tetracycline antibiotics and found that, in rats, anhydrotetracycline, anhydrochlortetracycline, and 9-/-butylanhydrochlortetracycline were all less active than oxytetracycline. Anderson and Anderson (1950) reported that chlortetracycline freed 3/3 naturally infected macaques of E. histolytica, but all three died after about a month. found few to survive dosage with chlor-or oxytetracycline, and though some of the animals which died were cleared of amebae, they concluded that neither had any significant therapeutic effect.…”

Section: Streptomycinmentioning

confidence: 99%

Chemotherapy of Amebiasis

Woolfe¹

1963

Experimental Chemotherapy

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“…Following the method recently reported by Hrenoff and Nakamura (1951) and associates (Anderson et al, 1950), the antibiotic was studied in 19 monkeys naturally infected with E. histolytica. The range of oral dosage, duration and effectiveness of therapy, during a three-month follow-up period are given in Table I. Tolerance studies in infected animals were made to determine what toxic or other untoward effects might be anticipated in man.…”

Section: Fumagillin In Monkey Amebiasismentioning

confidence: 99%

Fumagillin in Amebiasis 1

Anderson¹,

Hrenoff²,

Anderson³

et al. 1952

The American Journal of Tropical Medicine and Hygiene

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Fumagillin was isolated from Aspergillus fumigatus (NNRL 171) by Hanson and Eble (1949). It was first described as an antiphage agent. While the chemical composition is not known, it does occur in crystals and has been reported to contain only carbon, hydrogen and oxygen. This antibiotic has shown slight antibacterial or antifungal activity. Its chief attribute as a direct acting amebi cide was demonstrated by McCowen, Callender and Lawlis (1951). Against Endamoeba histolytica in vitro, fumagillin was reported active at one to four BURBIDGE, EARL. Personal Communication.

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Antibiotics against Amebiasis in Macaques 1,2

Cited by 10 publications

References 0 publications

Imaging hydroxyapatite in sub-retinal pigment epithelial deposits by fluorescence lifetime imaging microscopy with tetracycline staining

Imaging hydroxyapatite in sub-retinal pigment epithelial deposits by fluorescence lifetime imaging microscopy with tetracycline staining

Chemotherapy of Amebiasis

Fumagillin in Amebiasis 1

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