Antibiotics from Insect-Associated Actinobacteria

Baranova, Anna A.; Zakalyukina, Yu. V.; Ovcharenko, Anna A.; Korshun, Vladimir A.; Tyurin, Anton P.

doi:10.3390/biology11111676

Cited by 16 publications

(12 citation statements)

References 106 publications

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“…Squamellaria tissue) samples. According to Baranova et al [39], CA and GSA are among the most utilized isolation media for the isolation of actinomycetes from insect samples. Similarly, AIA and HVA are commonly used in the isolation of actinomycetes from environmental samples.…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization and screening of actinomycetes associated with fijian ant–plant symbioses

Diarra,

Osborne-Naikatini

2023

Microbiology

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In the search for novel therapeutics to combat the ongoing antimicrobial resistance crisis, scientists are turning to underexplored environments. Defensive mutualisms between hymenopteran insects and actinomycetes represent important reservoirs for bioactive compounds. In this study, we examined the association between actinomycetes and Squamellaria ant-plants spanning three different ant and plant species combinations (Squamellaria imberbis-Philidris nagasau, Squamellaria tenuiflora- Technomyrmex vitiensis, and Squamellaria tenuiflora-Tetramorium insolens). Eight Squamellaria plants were sampled including four containing T. vitiensis, three containing P. nagasau, and a single plant containing T. insolens. A total of 47 actinomycetes were obtained from the sampled material, with 5, 16, and 26 isolates originating from cuticle, tissue, and nest samples, respectively. Cross-streaking tests showed that 12 out of 47 isolates inhibited bacterial pathogens. The most frequently inhibited pathogens in the cross-streaking tests were S. aureus and E. coli while S. enterica was the least inhibited. Among the three primary screening media used, ISP2 agar was the most suitable for secondary metabolism as more isolates exhibited antibacterial activity when grown on the medium. TFS2010 and TFS2003, which matched to Streptomyces gramineus (>99% similarity), were the most bioactive isolates in cross-streaking tests. TFS2010 displayed the strong antibacterial on Nutrient agar, Mueller Hinton agar, and ISP2 agar while TFS2003 only exhibited strong antibacterial activity on Nutrient agar. Furthermore, a difference in potency of extracts based on batch culture medium was noted in TFS2010. DNA was extracted from 19 isolates and followed by 16SrRNA gene sequencing. Analysis of sequence data revealed the presence of six genera, including Amycolatopsis , Asanoa , Jiangella , Nocardia , Nocardiopsis , and Streptomyces , with the latter being the most abundant taxon. Among these, three isolates (PNS3002, PNS3005, and TFS3001) are likely to represent new species while one (TFS2015) is likely to be a member of a novel genus. Our work represents the first attempt to study actinomycetes from Squamellaria-ant mutualisms.

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Section: Discussionmentioning

confidence: 99%

Isolation, characterization and screening of actinomycetes associated with fijian ant–plant symbioses

Diarra,

Osborne-Naikatini

2023

Microbiology

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“…Symbiotic bacteria strongly associated with insects, such as Wolbachia Hertig, 1936, have a long history of study and a great potential for the regulation of insect populations. Coexistence of the macro-and microorganisms is possible through close interactions and an intensive exchange of chemical signals [Baranova et al, 2022]. However, it is diffi cult to prove connections and mutually benefi cial ecological relationships between insects and actinobacteria.…”

Section: Discussionmentioning

confidence: 99%

“…with an ability to synthesize a previously undescribed antibiotic sceliphrolactam has also been isolated from the mud wasp Sceliphron caementarium (Drury, 1773) [Oh et al, 2011]. There is an increasing number of recent reports about the isolation of mycelial prokaryote strains from various insects [Baranova et al, 2022], and ants remain the most popular object in this respect. Indeed, it was possible to show the presence of mycelial actinobacteria capable of synthesizing broad-spectrum antibiotics in adult individuals of carpenter and harvester ants [Zakalyukina et al, 2019[Zakalyukina et al, , 2022aBaranova et al, 2020].…”

Section: Introductionmentioning

confidence: 99%

Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)

Zakalyukina,

Alferova,

Biryukov

2023

euroasentj

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A collection of mycelial bacteria of the phylum Actinomycetota was isolated from adult working individuals o f the ant Lasius niger (Linnaeus, 1758) selected on the territory of Moscow by plating on nutrient media. It is shown that cultured members of actinomycete communities are confi ned to the heads of insects, and belong to the genera Nocardia Trevisan, 1889 and Streptomyces Waksman and Henrici, 1943. Among the strains isolated from ants within the same family, producers of antibacterial compounds prevail over antifungal ones. Actinomycetes with antibacterial activity inhibit development of entomopathogenic bacteria Bacillus thuringiensis VKPM B-6650 and Paenibacillus alvei VKM B-502, as well as growth of the o pportunistic bacterium Staphylococcus aureus ATCC 25923, representing one of the most important human pathogens worldwide. At the same time, ability of individual strains to restrain the growth of entomopathogenic fungi Beauveria bassiana VKPM F-1357, Conidiobolus coronatus VKPM F-1359, as well as clinic ally signifi cant yeast Candida albicans ATCC 14053 was noted. Further studies are required to clarify the localization of possible symbionts and their ability to synthesize antimicrobial metabolites in vivo. РЕЗЮМЕ. Из взрослых рабочих особей муравья Lasius niger (Linnaeus, 1978), отобранных на территории г. Москвы, методом посева на питательные среды выделена коллекция мицелиальных представителей филума Actinomycetota. Показано, что культивируемые участники актиномицетных сообществ приурочены к головному отделу насекомых, а в таксономическом отношении представлены родами Nocardia Trevisan, 1889 и Streptomyces Waksman et Henrici, 1943. Среди штаммов, выделенных из муравьев внутри одной семьи, превалируют продуценты антибактериальных, а не антигрибных соединений. Актиномицеты с антибактериальной активностью подавляют развитие энтомопатогенных бактерий Bacillus thuringiensis VKPM B-6650 и Paenibacillus alvei VKM B-502, а также рост оппортунистической бактерии Staphylococcus aureus ATCC 25923, одного из наиболее важных патогенов человека по всему миру. В то же время отмечена способность отдельных штаммов к сдерживанию роста энтомопатогенных грибов Beauveria bassiana VKPM F-1357, Conidiobolus coronatus VKPM F-1359, а также клинически значимых дрожжей Candida albicans ATCC 14053. Требуются дальнейшие исследования для уточнения локализации возможных симбионтов и способности их синтезировать антимикробные метаболиты in vivo.

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“…Chevrette et al [ 56 ] asserted that the strains of Streptomyces extracted from insects have more antibacterial activity than that of the strains found in soil or on plants. Baranova et al [ 57 ] gave an account of the techniques to extract Actinomycetes from insects and on the antimicrobial activity of the chemical compounds produced by these microorganisms.…”

Section: The Eusocial Waspsmentioning

confidence: 99%

Mutualistic Relationships between Microorganisms and Eusocial Wasps (Hymenoptera, Vespidae)

2023

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Eusocial wasps are represented in the Vespidae by the subfamilies Stenogastrinae, Vespinae and Polistinae. These wasps present colonies that are sometimes composed of thousands of individuals which live in nests built with paper materials. The high density of the adult and larval population, as well as the stable micro environment of the nests, make very favourable conditions for the flourishing of various types of microorganisms. These microorganisms, which may be pathogens, are beneficial and certainly contribute to model the sociality of these insects. The mutualistic relationships that we observe in some species, especially in Actinomycete bacteria and yeasts, could have important fallouts for the development of new medicines and for the use of these insects in agricultural environments.

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Antibiotics from Insect-Associated Actinobacteria

Cited by 16 publications

References 106 publications

Isolation, characterization and screening of actinomycetes associated with fijian ant–plant symbioses

Isolation, characterization and screening of actinomycetes associated with fijian ant–plant symbioses

Probable protective mission of actinomycetes associated with black garden ants, Lasius niger (Linnaeus, 1758) (Hymenoptera: Formicidae)

Mutualistic Relationships between Microorganisms and Eusocial Wasps (Hymenoptera, Vespidae)

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