Antibodies against &lt;i&gt;Aspergillus fumigatus&lt;/i&gt;

Kauffman, Henk F.; Vries, K. de

doi:10.1159/000232521

Cited by 47 publications

(4 citation statements)

References 10 publications

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“…The results indicated that higher carbon source concentrations in the medium (e.g., glucose) result in higher amounts of total nitrogen, soluble proteins, total carbohydrates and soluble carbohydrates in the allergenic extracts [86]. Similar effects were shown in the study by Kauffman et al (1980) [87], where higher glucose concentrations in the medium resulted in higher yields of A. fumigatus mycelium mass [87].…”

Section: Cultivation Mediumsupporting

confidence: 71%

“…Different studies have shown that the type of medium (i.e., liquid or solid medium) as well as the medium composition used for fungal cultivation can play an important role for the quality and quantity of fungal raw material obtained for the preparation of fungal extracts [52,78,[83][84][85][86][87][88][89].…”

Section: Cultivation Mediummentioning

confidence: 99%

“…In addition, individual components of the cultivation medium, such as the carbon source, can have an impact on fungal growth and therefore also potentially on fungal allergen expression [83,86,87]. In the study presented by Flaherty et al (1970), Trichophyton rubrum was cultivated using different concentrations of glucose to analyze the influence of the carbon source on the allergenicity of fungal extracts prepared from mycelium [86].…”

Section: Cultivation Mediummentioning

confidence: 99%

“…It has been shown that the ideal growth period of fungi to produce allergen extracts is highly species-specific, but usually 2-4 weeks of growth are optimal, because after this time most species have produced spores and sufficient amounts of mycelial material [95]. The impact of the cultivation time on the expression of fungal allergens and on their presence in the produced protein extracts has been extensively studied for A. fumigatus [9,82,87,96].…”

Section: Growth Timementioning

confidence: 99%

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Problems Encountered Using Fungal Extracts as Test Solutions for Fungal Allergy Diagnosis

Pfeiffer,

Swoboda

2023

JoF

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Fungal allergy is a worldwide public health burden, and problems associated with a reliable allergy diagnosis are far from being solved. Especially, the lack of high-quality standardized fungal extracts contributes to the underdiagnosis of fungal allergy. Compared to the manufacturing processes of extracts from other allergen sources, the processes used to manufacture extracts from fungi show the highest variability. The reasons for the high variability are manifold as the starting material, the growth conditions, the protein extraction methods, and the storage conditions all have an influence on the presence and quantity of individual allergens. Despite the vast variety of studies that have analyzed the impact of the different production steps on the allergenicity of fungal allergen extracts, much remains unknown. This review points to the need for further research in the field of fungal allergology, for standardization and for generally accepted guidelines on the preparation of fungal allergen extracts. In particular, the standardization of fungal extracts has been and will continue to be difficult, but it will be crucial for improving allergy diagnosis and therapy.

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