Antibodies to cellular antigens in Sjögren's syndrome.

Alspaugh, Margaret A.; Tan, Eng M.

doi:10.1172/jci108007

Cited by 397 publications

(151 citation statements)

References 22 publications

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“…Autoantibodies against components of the SS-A/Ro complexes include those recognizing a 60-kDa protein (Ro60), a 52-kDa protein (Ro52-␣), and a 45-kDa protein (Ro52-␤) (23)(24)(25)(26). A molecular definition for these autoantigens has been provided by the isolation of cDNA clones (25,27).…”

Section: Discussionmentioning

confidence: 99%

SS-A/Ro52, an Autoantigen Involved in CD28-Mediated IL-2 Production

Ishii

Ohnuma

Murakami

et al. 2003

The Journal of Immunology

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An autoantibody against SS-A/Ro52 (Ro52) is most frequently found in the sera of patients with Sjögren’s syndrome, systemic lupus erythematosus, and congenital heart block from anti-Ro52 Ab-positive mother. However, the physiological function of the autoantigen SS-A/Ro52 has not yet been elucidated. In this study, we describe the role of Ro52 protein in T cell activation. Overexpression of SS-A/Ro52 in Jurkat T cell resulted in enhanced IL-2 production following CD28 stimulation. Furthermore, transfection of anti-Ro52-specific small RNA duplexes partially blocked the expression of native and overexpressed Ro52 in Jurkat T cell, resulting in decreased IL-2 production via CD28 pathway in these cells. Finally, intracellular localization of Ro52 dramatically changed following CD28 stimulation. Our data reveal a novel function of Ro52 in CD28-mediated pathway, which eventually contributes to cytokine production and expression of the T cell biological programs.

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Section: Discussionmentioning

confidence: 99%

SS-A/Ro52, an Autoantigen Involved in CD28-Mediated IL-2 Production

Ishii

Ohnuma

Murakami

et al. 2003

The Journal of Immunology

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“…Rogers, AR) (3,4). The antibodies to SS-A and SS-B were determined using an extract of Wi12 cells prepared from cultures sustained in the laboratory (8).…”

Section: Methodsmentioning

confidence: 99%

The incidence of sjögren's sicca complex in a population of patients with keratoconjunctivitis sicca

Forstot¹,

Forstot²,

Greer

et al. 1982

Arthritis & Rheumatism

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A prospective study was performed on patients who came to a university ophthalmology outpatient clinic with the complaint of dry eyes and who were documented to have keratoconjunctivitis sicca (KCS). Of 45 patients studied 1 had primary amyloidosis, and another 10 had KCS with a previously diagnosed connective tissue disease. Of the remaining 34 patients, 19 had KCS alone without xerostomia, and the remaining 15 had KCS in association with subjective or objective xerostomia. Eight of these 15 patients were subsequently shown to have clinical Sjögren's syndrome–sicca complex, and for the majority, serologic and biopsy findings supported this diagnosis.

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“…They were then tested for precipitating antibodies by a modification of the Ouchterlony double-diffusion technique (3 1). Sera were investigated by immunodiffusion in agarose against rabbit thymus extract for antibodies to Sm and nuclear RNP (3,31), against soluble nucleoprotein for antibodies to sNP (32), and against WiL-2 extract for antibodies to SS-A and SS-B (12). Antibodies to double-and single-stranded DNA were measured by the Millipore filter technique (Millipore Corp., Bedford, Massachusetts) (7,33).…”

mentioning

confidence: 99%

Characteristics of antinuclear antibodies in rheumatoid. arthritis

Aitcheson

Peebles

Joslin

et al. 1980

Arthritis & Rheumatism

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The immunologic specificities of antinuclear antibodies (ANA) in sera from 97 patients with rheumatoid arthritis (RA) were studied. The frequency of ANA detected by immunofluorescence with mouse kidney sections as substrate was 60% (59/97) compared to 13% (7/ 55) in controls. IgM ANA was positive in 41% (40/97) of RA sera, IgG ANA in 40% (39/97), and IgA ANA in 33% (32/97). Specificities of antibodies in the ANA positive sera were examined by radioimmunoassay for antibodies to DNA and by immunodiffusion for antibodies to Sm antigen, nuclear ribonucleoprotein, and the SS-A and SS-B nuclear antigens. Antibodies to these nuclear antigens were found infrequently (1.7% to 6.8%). Sera were further investigated for antibodies to histones by an immunofluorescent method previously described using acid-extracted and histone reconstituted tissue sections. Twenty-four percent of the ANA positive sera (14/55) reacted with a histone-dependent nuclear antigen. The relationship of rheumatoid factor to ANA was studied by isolating rheumatoid factors (RF) from aggregated IgG absorbant columns. Ten of 19 isolated RF showed ANA activity, which did not result from complexes of rheumatoid factor with IgG ANA since IgM rheumatoid factor, dissociated from IgG under acid con- ditions, continued to show ANA activity. In 8 of 16 RA sera tested, both ANA and rheumatoid factor activity could be inhibited by aggregated IgG. Five of the crossreacting rheumatoid factors reacted specifically with a histone-dependent antigen in the reconstitution assay. These findings show that approximately WO of rheumatoid factors possess cross-reactivity with nuclear components, and histones are involved in a significant number of these reactions.

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Antibodies to cellular antigens in Sjögren's syndrome.

Cited by 397 publications

References 22 publications

SS-A/Ro52, an Autoantigen Involved in CD28-Mediated IL-2 Production

SS-A/Ro52, an Autoantigen Involved in CD28-Mediated IL-2 Production

The incidence of sjögren's sicca complex in a population of patients with keratoconjunctivitis sicca

Characteristics of antinuclear antibodies in rheumatoid. arthritis

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