Antibodies to dietary antigens in rheumatoid arthritis — possible molecular mimicry mechanism

Pérez-Maceda, B. T.; López‐Bote, Juan Pedro; Langa, Carmen; Bernabeu, Carmelo

doi:10.1016/0009-8981(91)90287-m

Cited by 31 publications

(18 citation statements)

References 44 publications

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“…In this case, antibodies directed to foreign protein antigens find similar antigen sites in the joint tissues, attacking these tissues. The amino acid residues 141-157 of bovine albumin have been identified as essentially the same as the amino acids found in human collagen in the joints (Perez-Maceda et al, 1991).…”

Section: Discussionmentioning

confidence: 82%

“…Elevated levels of antibodies to gut bacteria and to food have been found in people with various forms of inflammatory arthritis (Tiwana et al, 1997;Perez-Maceda et al, 1991). Large immune complexes can form in the blood and be filtered by the small capillaries supplying the joint capsule, causing an inflammatory reaction in the joint tissues (Danning et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

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Effects of a Very Low-Fat, Vegan Diet in Subjects with Rheumatoid Arthritis

McDougall

Bruce

Spiller

et al. 2002

The Journal of Alternative and Complementary Medicine

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Objective: To demonstrate the effects of a very low-fat, vegan diet on patients with rheumatoid arthritis (RA).Design: Single-blind dietary intervention study. Subjects and study interventions:This study evaluated the influence of a 4-week, very lowfat (,10%), vegan diet on 24 free-living subjects with RA, average age, 56 6 11 years old.Outcome measurements: Prestudy and poststudy assessment of RA symptomatology was performed by a rheumatologist blind to the study design. Biochemical measures and 4-day diet data were also collected. Subjects met weekly for diet instruction, compliance monitoring, and progress assessments.Results: There were significant (p , 0.001) decreases in fat (69%), protein (24%), and energy (22%), and a significant increase in carbohydrate (55%) intake. All measures of RA symptomatology decreased significantly (p , 0.05), except for duration of morning stiffness (p . 0.05). Weight also decreased significantly (p , 0.001). At 4 weeks, C-reactive protein decreased 16% (ns, p . 0.05), RA factor decreased 10% (ns, p . 0.05), while erythrocyte sedimentation rate was unchanged (p . 0.05).Conclusion: This study showed that patients with moderate-to-severe RA, who switch to a very low-fat, vegan diet can experience significant reductions in RA symptoms. 71

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Effects of a Very Low-Fat, Vegan Diet in Subjects with Rheumatoid Arthritis

McDougall

Bruce

Spiller

et al. 2002

The Journal of Alternative and Complementary Medicine

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“…Collectively, these observations associate MS with abnormal T cell immunity to common environmental food Ags in cow milk, and they bring to three the number of autoimmune disorders with abnormal immunity to BSA: T1DM, rheumatoid arthritis, and MS (26,48,49). In MS, this abnormality might contribute to the association of high cow milk exposure and the risk to develop MS or its relapses (11)(12)(13)50).…”

Section: Resultsmentioning

confidence: 99%

T Cells of Multiple Sclerosis Patients Target a Common Environmental Peptide that Causes Encephalitis in Mice

Winer

Astsaturov

Cheung

et al. 2001

The Journal of Immunology

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Multiple sclerosis (MS) is a chronic autoimmune disease triggered by unknown environmental factors in genetically susceptible hosts. MS risk was linked to high rates of cow milk protein (CMP) consumption, reminiscent of a similar association in autoimmune diabetes. A recent rodent study showed that immune responses to the CMP, butyrophilin, can lead to encephalitis through antigenic mimicry with myelin oligodendrocyte glycoprotein. In this study, we show abnormal T cell immunity to several other CMPs in MS patients comparable to that in diabetics. Limited epitope mapping with the milk protein BSA identified one specific epitope, BSA193, which was targeted by most MS but not diabetes patients. BSA193 was encephalitogenic in SJL/J mice subjected to a standard protocol for the induction of experimental autoimmune encephalitis. These data extend the possible, immunological basis for the association of MS risk, CMP, and CNS autoimmunity. To pinpoint the same peptide, BSA193, in encephalitis-prone humans and rodents may imply a common endogenous ligand, targeted through antigenic mimicry.

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“…The presence of glycan chains in cusativin was studied using the Glycan Detection Kit from Boehringer (Mannheim, Germany) using transferrin (M~ 79500) as standard. Enzymelinked immunosorbent assay (ELISA) was performed essentially using the method outlined in P6rez-Maceda et al (1991) except that internal standards were included and the final concentration of protein was maintained constant at 200 ng per well (Metodiev and Demirevska-Kepova 1992). The RNase activity of cusativin on yeast RNA or polynucleotides was assessed at 37 ° C determining the cold-trichloroacetic acid-soluble material absorbing at 260 rim.…”

Section: Generation Of the Rip-diagnostic Rna Fragment With Single-chmentioning

confidence: 99%

Cusativin, a new cytidine-specific ribonuclease accumulated in seeds of Cucumis sativus L.

Arias

Iglesias

et al. 1994

Planta

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Dry seeds of Cucumis sativus L. were found to contain a heat-sensitive endoribonuclease of a novel type which we have named cusativin. It was purified to apparent electrophoretic homogeneity by chromatography through S-Sepharose Fast Flow, Sephadex G-75, CM-Sepharose, Superdex 75-FPLC (fast protein liquid chromatography) and Mono S-FPLC. It is a single unglycosylated polypeptide chain with an apparent molecular mass (M(r)) of 22900. Polyclonal anti-cusativin antibodies raised in rabbits only reacted with melonin, the translation inhibitor from Cucumis melo L. Functional, Western blot and enzyme-linked immunosorbent assay (ELISA) analyses indicated that cusativin is present in the coat and cotyledons of dry seeds, but not in embryonic axes. Cusativin is accumulated in maturing seeds. By contrast, after seed germination there is degradation of the cusativin present in cotyledons but not that present in the seed coat. The preference of cusativin for polynucleotide cleavage was poly(C) >> poly(A) acids, poly(U) and poly(G) being unaffected by cusativin. Under the denaturing conditions used for RNA sequencing, cusativin acted only on poly(C). Cusativin proved to be useful for RNA sequencing, in particular, complementing the data obtained with RNase CL3. Cusativin represents a new class of plant RNase and, as far as we are aware, is the first plant enzyme that shows cleavage specificity for cytidine under the denaturing conditions of RNA sequencing.

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Antibodies to dietary antigens in rheumatoid arthritis — possible molecular mimicry mechanism

Cited by 31 publications

References 44 publications

Effects of a Very Low-Fat, Vegan Diet in Subjects with Rheumatoid Arthritis

Effects of a Very Low-Fat, Vegan Diet in Subjects with Rheumatoid Arthritis

T Cells of Multiple Sclerosis Patients Target a Common Environmental Peptide that Causes Encephalitis in Mice

Cusativin, a new cytidine-specific ribonuclease accumulated in seeds of Cucumis sativus L.

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