Antibodies to Intermediate Filament Proteins

Miettinen, Markku; Lehto, Veli‐Pekka; Virtanen, Ismo

doi:10.1001/archderm.1985.01660060050019

Cited by 41 publications

(4 citation statements)

References 34 publications

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“…Desmin is an important marker protein made use of in the immunohistochemical diagnosis of the histogenesis of tumours of connective and supporting tissues [e.g. 11,14]. This protein has a dynamic structure and can migrate to other sites, and there reorganize itself within a few minutes, its regulation being controlled by accompanying proteins [15,19].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical investigations to demonstrate vital direct traumatic damage of skeletal muscle

et al. 1991

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The muscle proteins actin, myosin, desmin and myoglobin were investigated in traumatically damaged human and animal skeletal muscle using an immunohistochemical PAP-method. A depletion of all the proteins investigated was observed in muscle fibres damaged in the antemortem period. The antigens could however also be demonstrated in the otherwise empty sarcolemma, the discoid disintegration zones of the fibres and between the fibres. The depletion begins immediately after the trauma and myoglobin is the first to be affected. No such changes could be observed after post mortem muscle damage. The antigens could be demonstrated until 72 hours post mortem. The demonstration of protein depletion is an important addition to the light microscopical findings in vital muscle alterations.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical investigations to demonstrate vital direct traumatic damage of skeletal muscle

et al. 1991

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“…According to their report, glomus cells positively reacted to desmin by the avidin-biotin (ABC) immunoperoxidase method. On the other hand, Miettinen et al (8) found that these cells reacted positively to vimentin and negatively to desmin in all 7 cases tested. Smooth muscle cells usually react negatively to vimentin.…”

Section: Case Reportmentioning

confidence: 90%

Localized Form of Multiple Glomus Tumors: Report of the First Case Showing Partial Involution

Kato

Kumakiri

Ohkawara

1990

The Journal of Dermatology

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We recently examined a boy with relatively large multiple glomus tumors on the left scapular region. Histologic examination revealed a typical non-capsulated glomangioma in the middle and deep dermis; by electron microscopic examination, the tumor cells were seen to exhibit a characteristic smooth muscle cell structure. Thermographic examination revealed the higher temperature of the tumor. Within a year, the tumor showed a partial involution. We think this is the first report of multiple glomus tumor showing a partial involution.

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“…Previously reported studies of BCC and SCC using antikeratin immunohistochemical techniques have not included large numbers of BCC and SCC and usually were retrospective ones that utilized small superficial biopsy specimens already on file in the collections of surgical pathology 5,16,33–39 . These specimens were fixed in formalin containing fixative 13,33–35,39 prior to paraffin embedding, in absolute alcohol, or Zenker's solution as a means of preserving the intermediate filaments 40 . In those instances when fresh frozen material was used, the type of BCC or SCC was not reported 13,20,23,35,36 .…”

Section: Discussionmentioning

confidence: 99%

Expression of Keratin Proteins in Deeply Invasive Basal and Squamous Cell Carcinoma: An Immunohistochemical Study

Robinson

1987

The Journal of Dermatologic Surgery and Oncology

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The expression of certain classes of keratin is associated with cell maturation and differentiation. During cell transformation and tumor development, the cell specificity of intermediate filament, keratin, is largely conserved. Taking advantage of this, we utilized monoclonal antikeratin immunohistochemical techniques to examine basal and squamous cell carcinomas as they became deeply invasive. Dyskeratotic keratinocytes and keratin pearls in squamous cell carcinomas (SCCs) stain with antikeratin antisera to larger keratins (65-67 Kd). At the deep tumor margins, SCCs no longer express larger keratins but retain expression of 50, 58 Kd, which are markers of keratinocytes derived from stratified squamous epithelial cells. This selective loss of expression of keratin polypeptide markers of differentiation in SCC is associated with progressively more aggressive biologic behavior as the tumor invades deeper structures such as muscle and bone. Recurrent basal cell carcinoma (BCC) which was of the nodular type when first excised, shows features of morphea-like BCC and of aggressive growth patterns at the deep invasive margin. At these deep margins, some tumors express markers of keratinization (65-67 Kd). While tumor cells retain the specificity of the intermediate filament, keratin, the individual cells express products of differentiation as measured by keratin expression independently of their cytologic atypia. At the deeper invasive margin of the tumor, the neoplastic cells synthesize keratin proteins in an unpredictable fashion.

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Antibodies to Intermediate Filament Proteins

Cited by 41 publications

References 34 publications

Immunohistochemical investigations to demonstrate vital direct traumatic damage of skeletal muscle

Immunohistochemical investigations to demonstrate vital direct traumatic damage of skeletal muscle

Localized Form of Multiple Glomus Tumors: Report of the First Case Showing Partial Involution

Expression of Keratin Proteins in Deeply Invasive Basal and Squamous Cell Carcinoma: An Immunohistochemical Study

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